| Catalog Number | CL488-67455 |
| Synonyms | 3C6A6, CCTD, CCT-delta, Chaperonin containing T-complex polypeptide 1 subunit 4, SRB |
| Host | Mouse |
| Reactivity | human, mouse, pig, rabbit |
| Form | Liquid |
| Formulation | PBS, Proclin300, BSA, Glycerol |
| Applications | IF/ICC, FC (Intra) |
| Host / Isotype | Mouse / IgG1 |
| Tested Applications — Positive IF/ICC detected in | U2OS cells |
| Tested Applications — Positive FC (Intra) detected in | A549 cells |
| Recommended Dilutions — Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Recommended Dilutions — Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension |
| Positive IF/ICC detected in | U2OS cells |
| Positive FC (Intra) detected in | A549 cells |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension |
| Tested Reactivity | human, mouse, pig, rabbit |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | CatNo: Ag17084 Product name: Recombinant human CCT4 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 108-407 aa of BC106934 Sequence: SVVIIAGSLLDSCTKLLQKGIHPTIISESFQKALEKGIEILTDMSRPVELSDRETLLNSATTSLNSKVVSQYSSLLSPMSVNAVMKVIDPATATSVDLRDIKIVKKLGGTIDDCELVEGLVLTQKVSNSGITRVEKAKIGLIQFCLSAPKTDMDNQIVVSDYAQMDRVLREERAYILNLVKQIKKTGCNVLLIQKSILRDALSDLALHFLNKMKIMVIKDIEREDIEFICKTIGTKPVAHIDQFTADMLGSAELAEEVNLNGSGKLLKITGCASPGKTVTIVVRGSNKLVIEEAERSIHD Predict reactive species |
| Full Name | chaperonin containing TCP1, subunit 4 (delta) |
| Calculated Molecular Weight | 539 aa, 58 kDa |
| Observed Molecular Weight | 55-58 kDa |
| GenBank Accession Number | BC106934 |
| Gene Symbol | CCT4 |
| Gene ID (NCBI) | 10575 |
| RRID | AB_3672948 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
| Excitation Laser | Blue laser (488 nm) |
| Purification Method | Protein G purification |
| UNIPROT ID | P50991 |
| Storage Buffer | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. |
| FC protocol for CL Plus 488 CCT4 antibody CL488-67455 | Download protocol |
| IF protocol for CL Plus 488 CCT4 antibody CL488-67455 | Download protocol |
| Citations | - |
| Dilutions | IF/ICC : 1:50-1:500 FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Clone Number | 3C6A6 |
| Conjugation | CoraLite® Plus 488 |
T-complex protein 1 subunit delta (CCT4) is a component of the chaperonin-containing T-complex (TRiC), a molecular chaperone complex that assists the folding of proteins upon ATP hydrolysis (PMID:25467444). As part of the TRiC complex, it may play a role in regulating telomere maintenance (PMID:25467444) and assembly of BBSome, a complex involved in ciliogenesis regulating transports vesicles to the cilia (PMID:20080638). CCT4 has 2 isoforms produced by alternative splicing with the MW of 55 kDa and 58 kDa. Protocols Product Specific Protocols FC protocol for CL Plus 488 CCT4 antibody CL488-67455 Download protocol IF protocol for CL Plus 488 CCT4 antibody CL488-67455 Download protocol Standard Protocols Click here to view our Standard Protocols