BNIP3L Polyclonal antibody 12986-1-AP proteintech
$149.00
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SKU
12986-1-AP
| Catalog Number | 12986-1-AP |
| Synonyms | Adenovirus E1B19K-binding protein B5, BCL2/adenovirus E1B 19 kDa protein-interacting protein 3A, BNIP3A, BNIP3H, NIP3L |
| Host | Rabbit |
| Reactivity | human, mouse, rat and More (2) |
| Reactivity | human, mouse, rat, pig, zebrafish |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, IHC, IF/ICC, ELISA |
| Host / Isotype | Rabbit / IgG |
| Tested Applications — Positive WB detected in | HEK-293 cells, Raji cells, mouse kidney tissue, Jurkat cells, rat kidney tissue |
| Tested Applications — Positive IHC detected in | human skin cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Tested Applications — Positive IF/ICC detected in | U2OS cells |
| Recommended Dilutions — Western Blot (WB) | WB : 1:500-1:3000 |
| Recommended Dilutions — Immunohistochemistry (IHC) | IHC : 1:150-1:600 |
| Recommended Dilutions — Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| Positive WB detected in | HEK-293 cells, Raji cells, mouse kidney tissue, Jurkat cells, rat kidney tissue |
| Positive IHC detected in | human skin cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | U2OS cells |
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunohistochemistry (IHC) | IHC : 1:150-1:600 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, pig, zebrafish |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CatNo: Ag3624 Product name: Recombinant human BNIP3L protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-219 aa of BC001559 Sequence: MSSHLVEPPPPLHNNNNNCEENEQSLPPPAGLNSSWVELPMNSSNGNDNGNGKNGGLEHVPSSSSIHNGDMEKILLDAQHESGQSSSRGSSHCDSPSPQEDGQIMFDVEMHTSRDHSSQSEEEVVEGEKEVEALKKSADWVSDWSSRPENIPPKEFHFRHPKRSVSLSMRKSGAMKKGGIFSAEFLKVFIPSLFLSHVLALGLGIYIGKRLSTPSASTY Predict reactive species |
| Full Name | BCL2/adenovirus E1B 19kDa interacting protein 3-like |
| Calculated Molecular Weight | 24 kDa |
| Observed Molecular Weight | 70 kDa |
| GenBank Accession Number | BC001559 |
| Gene Symbol | BNIP3L |
| Gene ID (NCBI) | 665 |
| RRID | AB_2877901 |
| Conjugate | Unconjugated |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O60238 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| IF protocol for BNIP3L antibody 12986-1-AP | Download protocol |
| IHC protocol for BNIP3L antibody 12986-1-AP | Download protocol |
| WB protocol for BNIP3L antibody 12986-1-AP | Download protocol |
| human | IHC Neuromuscul Disord Mitophagy in three cases of immune-mediated necrotizing myopathy associated with anti-3-hydroxy-3-methylglutaryl-coenzyme A reductase autoantibodies: ultrastructural and immunohistochemical studies. Authors - Shiro Matsubara View Article |
| mouse | WB Cell Rep Mitophagy Directs Muscle-Adipose Crosstalk to Alleviate Dietary Obesity. Authors - Tingting Fu View Article |
| Citations | 53 |
| Dilutions | WB : 1:500-1:3000 IHC : 1:150-1:600 IF/ICC : 1:200-1:800 |
| Isotype | IgG |
| Clonality | Polyclonal |
| Clone Number | - |
| Conjugation | Unconjugated |
BNIP3L, also named as BNIP3A, BNIP3H, NIX and NIP3L. Bnip3L is one of the BH3-containing pro-apoptotic proteins and that it targets the mitochondria when inducing apoptosis. It may function as a tumor suppressor. BNIP3L has Dimer form with MW ~70 kDa. Protocols Product Specific Protocols IF protocol for BNIP3L antibody 12986-1-AP Download protocol IHC protocol for BNIP3L antibody 12986-1-AP Download protocol WB protocol for BNIP3L antibody 12986-1-AP Download protocol Standard Protocols Click here to view our Standard Protocols
Publications
- Mitochondrial dysfunction and mitophagy defect triggered by heterozygous GBA mutations.
Journal: Autophagy | Authors: Hongyu Li | Species: human | Application: WB - Autophagy loss impedes cancer-associated fibroblast activation via downregulating proline biosynthesis.
Journal: Autophagy | Authors: Jingru Bai KO Validated | Species: mouse | Application: WB - Proline restores mitochondrial function and reverses aging hallmarks in senescent cells
Journal: Cell Rep | Authors: Debanik Choudhury | Species: human | Application: WB - Mitophagy Directs Muscle-Adipose Crosstalk to Alleviate Dietary Obesity.
Journal: Cell Rep | Authors: Tingting Fu | Species: mouse | Application: WB - Hyperosmolarity-Induced Oxidative Stress Leads to Senescence in Human Corneal Epithelial Cells (HCEPC) via DNA Damage, Metabolic Disturbance and Mitophagy Decline.
Journal: Antioxidants (Basel) | Authors: Yongjie Zhang | Species: human | Application: WB - Mitophagy in three cases of immune-mediated necrotizing myopathy associated with anti-3-hydroxy-3-methylglutaryl-coenzyme A reductase autoantibodies: ultrastructural and immunohistochemical studies.
Journal: Neuromuscul Disord | Authors: Shiro Matsubara | Species: human | Application: IHC - PABPC1 SUMOylation enhances cell survival by promoting mitophagy through stabilizing U-rich mRNAs within stress granules.
Journal: Nat Commun | Authors: Caihu Huang | Species: human | Application: WB - Fructooligosaccharides and ellagic acid synergistically enhance muscular endurance via targeting gut microbial urolithin A biosynthesis.
Journal: J Adv Res | Authors: Laiming Zhang | Species: mouse | Application: WB - MeCP2 dysregulation inhibits mitophagy and impairs neural development in cortical organoids.
Journal: J Adv Res | Authors: Jing Zhou | Species: human | Application: WB - Pharmacological induction of acetyl-CoA carboxylase 1 autophagic degradation attenuates lipid accumulation and cholangiocarcinoma progression.
Journal: J Exp Clin Cancer Res | Authors: Yani Pan | Species: mouse,human | Application: WB - Nanoparticle-based serine replenishment rescues SP1-BNIP3 mitophagy and ameliorates preeclampsia in preclinical models.
Journal: Cell Rep Med | Authors: Yinan Wang | Species: human, mouse | Application: WB - PINK1 overexpression suppresses p38 MAPK/NF‑κB signaling to attenuate chondrocyte senescence in osteoarthritis.
Journal: Int J Mol Med | Authors: Lishi Jie | Species: mouse,human | Application: WB - PI3K p85α/HIF-1α accelerates the development of pulmonary arterial hypertension by regulating fatty acid uptake and mitophagy
Journal: Mol Med | Authors: Chenyang Chen | Species: rat | Application: WB - Rubicon, a Key Molecule for Oxidative Stress-Mediated DNA Damage, in Ovarian Granulosa Cells
Journal: Antioxidants (Basel) | Authors: Kiyotaka Yamada | Species: human - Camellia japonica Flower Extract and the Active Constituent Hyperoside Repair DNA Damage Through FUNDC1-Mediated Mitophagy Pathway for Skin Anti-Aging
Journal: Antioxidants (Basel) | Authors: Hongqi Gao - Hyperosmolarity-Induced Oxidative Stress Leads to Senescence in Human Corneal Epithelial Cells (HCEPC) via DNA Damage, Metabolic Disturbance and Mitophagy Decline.
Journal: Antioxidants (Basel) | Authors: Yongjie Zhang | Species: human | Application: WB - Maintaining homeostasis of mitochondria and endoplasmic reticulum with NSC228155 alleviates cisplatin-induced acute kidney injury.
Journal: Free Radic Biol Med | Authors: Yanwei Li | Species: mouse | Application: WB - Inhibiting VDAC1 oligomerization attenuated cerebral ischemia-reperfusion injury by promoting mitophagy via reduced LONP1 interaction.
Journal: Free Radic Biol Med | Authors: Xiangna Guo - Proline restores mitochondrial function and reverses aging hallmarks in senescent cells
Journal: Cell Rep | Authors: Debanik Choudhury | Species: human | Application: WB - Mitophagy Directs Muscle-Adipose Crosstalk to Alleviate Dietary Obesity.
Journal: Cell Rep | Authors: Tingting Fu | Species: mouse | Application: WB - Rg1-R1 attenuates cardiac ischemia/reperfusion-induced endothelial cell injury through activating the ULK1/PGAM5-FUNDC1-mitophagy pathway.
Journal: J Ginseng Res | Authors: Xiyabin Song | Species: mouse | Application: WB - Deapioplatycodin D promotes cell senescence induced by P21 through the mediation of incomplete mitophagy via BNIP3L
Journal: Biomed Pharmacother | Authors: Yiquan Li | Species: mouse,human | Application: WB,IHC - Targeting DRP1 with Mdivi-1 to correct mitochondrial abnormalities in ADOA plus syndrome
Journal: JCI Insight | Authors: Yan Lin | Species: human | Application: WB - Anti-insulin resistance effects of salidroside through mitochondrial quality control.
Journal: J Endocrinol | Authors: Baiyang You | Species: mouse | Application: WB - In Vivo Metabolic Responses to Different Formulations of Amino Acid Mixtures for the Treatment of Phenylketonuria (PKU).
Journal: Int J Mol Sci | Authors: Nadia Giarratana | Species: rat | Application: WB - Mitophagy-mtROS axis contributes to anti-tuberculosis-induced liver injury through activation of the cGAS-STING pathway in rat hepatocytes
Journal: Int Immunopharmacol | Authors: Wenyan Chen | Species: rat | Application: WB - PINK1/Parkin-Mediated Mitophagy Regulation by Reactive Oxygen Species Alleviates Rocaglamide A-Induced Apoptosis in Pancreatic Cancer Cells.
Journal: Front Pharmacol | Authors: Chunle Zhao | Species: human | Application: WB - Tcap Deficiency in Zebrafish Leads to ROS Production and Mitophagy, and Idebenone Improves its Phenotypes.
Journal: Front Cell Dev Biol | Authors: Xiaoqing Lv | Species: zebrafish | Application: WB - Diosmetin alleviates hyperuricemic nephropathy by activating the BNIP3/Nrf2/GPX4 pathway to inhibit ferroptosis of renal tubular epithelial cells induced by monosodium urate crystals.
Journal: J Nutr Biochem | Authors: Chengming Hu | Species: human | Application: WB - HIF-1α/BNIP3L-mediated mitophagy is involved in T-2 toxin-induced myocardial injury.
Journal: Chem Biol Interact | Authors: Qian Li | Species: rat | Application: WB - Resveratrol Ameliorates Fibrosis in Rheumatoid Arthritis-Associated Interstitial Lung Disease via the Autophagy–Lysosome Pathway
Journal: Molecules | Authors: Lanxin Bao | Species: mouse | Application: WB - Capsaicin attenuates sepsis‑associated encephalopathy by inhibiting neuroinflammation and apoptosis whilst activating mitophagy through the BNIP3/NIX pathway
Journal: Mol Med Rep | Authors: Silun Zhang - Bone marrow mesenchymal stem cells modulate miR-202-3p to suppress neuronal apoptosis following spinal cord injury through autophagy activation via the AMPK, MAPK, and PI3K/AKT/mTOR signaling pathway
Journal: Sci Rep | Authors: Ke Huang | Species: rat | Application: WB - TRPM8-dependent protective effects of L-menthol attenuates lipid overload-induced calcium dysregulation and mitochondrial dysfunction in mouse ventricular myocytes.
Journal: J Mol Cell Cardiol | Authors: Xiang Sun | Species: mouse | Application: WB - Hydrogen Sulphide modulating mitochondrial morphology to promote mitophagy in endothelial cells under high-glucose and high-palmitate.
Journal: J Cell Mol Med | Authors: Ning Liu | Species: rat | Application: WB - Diltiazem Hydrochloride Protects Against Myocardial Ischemia/Reperfusion Injury in a BNIP3L/NIX-Mediated Mitophagy Manner
Journal: J Inflamm Res | Authors: Xing Zhou | Species: rat | Application: WB,IHC - A novel PHD2 inhibitor acteoside from Cistanche tubulosa induces skeletal muscle mitophagy to improve cancer-related fatigue.
Journal: Biomed Pharmacother | Authors: Shilei Zhang | Species: mouse | Application: WB - Salidroside Attenuates Cerebral Ischemia-Reperfusion Injury via ERβ/BNIP3-Mediated Mitochondrial Autophagy Activation in a Rat Model.
Journal: Neurochem Res | Authors: Xing Rong | Species: rat | Application: WB - Decidual protein induced by progesterone enhances HIF-1α stability to promote mitophagy and glycolysis.
Journal: FEBS J | Authors: Baopeng Tian | Species: human | Application: WB - Global Phosphoproteomic Analysis Reveals Significant Metabolic Reprogramming in the Termination of Liver Regeneration in Mice.
Journal: J Proteome Res | Authors: Jingzi Zhang | Species: mouse | Application: WB - BNIP3L-Mediated Mitophagy Inhibits Cadmium-Induced Apoptosis in the Rat Cerebral Cortex.
Journal: J Biochem Mol Toxicol | Authors: Liang Wang | Species: rat | Application: WB,IF - Mitophagy in three cases of immune-mediated necrotizing myopathy associated with anti-3-hydroxy-3-methylglutaryl-coenzyme A reductase autoantibodies: ultrastructural and immunohistochemical studies.
Journal: Neuromuscul Disord | Authors: Shiro Matsubara | Species: human | Application: IHC - Mitophagy in three cases of immune-mediated necrotizing myopathy associated with anti-3-hydroxy-3-methylglutaryl-coenzyme A reductase autoantibodies: ultrastructural and immunohistochemical studies.
Journal: Neuromuscul Disord | Authors: Shiro Matsubara | Species: human | Application: IHC - Mitochondrial dysfunction and mitophagy pathway activation in hepatitis E virus-infected livers of Mongolian gerbils.
Journal: Virus Res | Authors: Yifei Yang | Species: rat | Application: WB,IHC - The role of nasal microbiota and type 2 innate lymphoid cells in the pathogenesis of allergic rhinitis.
Journal: Biochem Biophys Rep | Authors: Chen Wang | Species: mouse | Application: WB - Combination treatment with antioxidants and creatine alleviates common and variant-specific mitochondrial impairments in Leber's hereditary optic neuropathy patient-derived fibroblasts.
Journal: Hum Mol Genet | Authors: Donald Xhuti | Species: human | Application: WB - EGR2 affects mixed stroke repair through BNIP3L-mediated microglial mitophagy.
Journal: IBRO Neurosci Rep | Authors: Xiujun Zhang | Species: mouse | Application: WB - The Mechanistic Study of Mitochondrial Autophagy and Ferroptosis in the Progression of Decreased Ovarian Reserve
Journal: Reprod Sci | Authors: Qianwen Ma | Species: rat | Application: WB - The Mechanistic Study of Mitochondrial Autophagy and Ferroptosis in the Progression of Decreased Ovarian Reserve
Journal: Reprod Sci | Authors: Qianwen Ma | Species: rat | Application: WB - CoCl2 -triggered pseudohypoxic stress induces proteasomal degradation of SIRT4 via polyubiquitination of lysines K78 and K299
Journal: FEBS Open Bio | Authors: Nils Hampel | Species: Human - Jionoside A1 alleviates ischemic stroke ischemia/reperfusion injury by promoting Nix-mediated mitophagy
Journal: Cell Mol Biol (Noisy-le-grand) | Authors: Xinyuan Yu - Ubiquitin ligase ITCH regulates life cycle of SARS-CoV-2 virus.
Journal: Elife | Authors: Qiwang Xiang | Species: human | Application: WB - Ubiquitin Ligase ITCH Regulates Life Cycle of SARS-CoV-2 Virus
Journal: bioRxiv | Authors: Qiwang Xiang | Application: WB
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