GSS Polyclonal antibody 15712-1-AP proteintech
$149.00
In stock
SKU
15712-1-AP
| Catalog Number | 15712-1-AP |
| Synonyms | EC:6.3.2.3, Glutathione synthase, glutathione synthetase, GSH S, GSH synthetase |
| Host | Rabbit |
| Reactivity | human, mouse, rat, pig, canine, hamster and More (2) |
| Reactivity | human, mouse, rat, pig, canine, hamster, monkey, chicken |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, IHC, IF/ICC, ELISA |
| Host / Isotype | Rabbit / IgG |
| Tested Applications — Positive WB detected in | CHO cells, HEK-293 cells, mouse liver tissue, rat liver tissue |
| Tested Applications — Positive IHC detected in | human kidney tissue, mouse kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Tested Applications — Positive IF/ICC detected in | MDCK cells |
| Recommended Dilutions — Western Blot (WB) | WB : 1:1000-1:4000 |
| Recommended Dilutions — Immunohistochemistry (IHC) | IHC : 1:100-1:400 |
| Recommended Dilutions — Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Positive WB detected in | CHO cells, HEK-293 cells, mouse liver tissue, rat liver tissue |
| Positive IHC detected in | human kidney tissue, mouse kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | MDCK cells |
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:100-1:400 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Tested Reactivity | human, mouse, rat, pig, canine, hamster |
| Cited Reactivity | human, mouse, rat, pig, monkey, chicken |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CatNo: Ag8338 Product name: Recombinant human GSS protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 125-474 aa of BC007927 Sequence: RSDYMFQRSADGSPALKQIEINTISASFGGLASRTPAVHRHVLSVLSKTKEAGKILSNNPSKGLALGIAKAWELYGSPNALVLLIAQEKERNIFDQRAIENELLARNIHVIRRTFEDISEKGSLDQDRRLFVDGQEIAVVYFRDGYMPRQYSLQNWEARLLLERSHAAKCPDIATQLAGTKKVQQELSRPGMLEMLLPGQPEAVARLRATFAGLYSLDVGEEGDQAIAEALAAPSRFVLKPQREGGGNNLYGEEMVQALKQLKDSEERASYILMEKIEPEPFENCLLRPGSPARVVQCISELGIFGVYVRQEKTLVMNKHVGHLLRTKAIEHADGGVAAGVAVLDNPYPV Predict reactive species |
| Full Name | glutathione synthetase |
| Calculated Molecular Weight | 474 aa, 52 kDa |
| Observed Molecular Weight | 52 kDa |
| GenBank Accession Number | BC007927 |
| Gene Symbol | GSS |
| Gene ID (NCBI) | 2937 |
| RRID | AB_2878171 |
| Conjugate | Unconjugated |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P48637 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| IF protocol for GSS antibody 15712-1-AP | Download protocol |
| IHC protocol for GSS antibody 15712-1-AP | Download protocol |
| WB protocol for GSS antibody 15712-1-AP | Download protocol |
| mouse,human | WB,IHC,IF ACS Nano Engineered Extracellular Vesicle-Delivered CRISPR/Cas9 for Radiotherapy Sensitization of Glioblastoma Authors - Xiao Liu View Article |
| human | WB Pharmacol Res Phyto-sesquiterpene lactones DET and DETD-35 induce ferroptosis in vemurafenib sensitive and resistant melanoma via GPX4 inhibition and metabolic reprogramming. Authors - Meng-Ting Chang View Article |
| mouse | WB FASEB J Taurine treatment prevents derangement of the hepatic γ-glutamyl cycle and methylglyoxal metabolism in a mouse model of classical homocystinuria: regulatory crosstalk between thiol and sulfinic acid metabolism. Authors - Kenneth N Maclean View Article |
| Hua (Verified Customer) (08-05-2021) | This is the second tube I purchased from ProteinTech. I got my first tube of this antibody in 2016 and it worked well. However, it suddenly stopped working last year. There is no signal at all on the membrane. Thus, I purchased the second tube in May 2021 and I just used it on my experiments as my previous record showing it is working well in WB. Unfortunately, I couldn't get any signal at all. I ran two tests with this antibody and it shows it stopping work with mouse liver. Applications: Western Blot Primary Antibody Dilution: 1:1000 Cell Tissue Type: Human liver, mouse liver, mouse brain and mouse kidney. GSS Antibody Western Blot validation (1:1000 dilution) in Human liver, mouse liver, mouse brain and mouse kidney. (Cat no:15712-1-AP) |
| Citations | 41 |
| Dilutions | WB : 1:1000-1:4000 IHC : 1:100-1:400 IF/ICC : 1:50-1:500 |
| Isotype | IgG |
| Clonality | Polyclonal |
| Clone Number | - |
| Conjugation | Unconjugated |
Publications
- Engineered Extracellular Vesicle-Delivered CRISPR/Cas9 for Radiotherapy Sensitization of Glioblastoma
Journal: ACS Nano | Authors: Xiao Liu | Species: mouse,human | Application: WB,IHC,IF - Paracrine Orchestration of Tumor Microenvironment Remodeling Induced by GLO1 Potentiates Lymph Node Metastasis in Breast Cancer
Journal: Adv Sci (Weinh) | Authors: Jindong Xie | Species: human | Application: WB - IMCA Induces Ferroptosis Mediated by SLC7A11 through the AMPK/mTOR Pathway in Colorectal Cancer.
Journal: Oxid Med Cell Longev | Authors: Lei Zhang | Species: human | Application: WB - γ-glutamylcysteine exhibits anti-inflammatory effects by increasing cellular glutathione level.
Journal: Redox Biol | Authors: Yang Yang | Species: mouse | Application: WB - Phyto-sesquiterpene lactones DET and DETD-35 induce ferroptosis in vemurafenib sensitive and resistant melanoma via GPX4 inhibition and metabolic reprogramming.
Journal: Pharmacol Res | Authors: Meng-Ting Chang | Species: human | Application: WB - Taurine treatment prevents derangement of the hepatic γ-glutamyl cycle and methylglyoxal metabolism in a mouse model of classical homocystinuria: regulatory crosstalk between thiol and sulfinic acid metabolism.
Journal: FASEB J | Authors: Kenneth N Maclean | Species: mouse | Application: WB - Exogenous H2S initiating Nrf2/GPx4/GSH pathway through promoting Syvn1-Keap1 interaction in diabetic hearts
Journal: Cell Death Discov | Authors: Mengyi Wang | Species: mouse | Application: WB - Cytochrome P450 1B1 directs pathogenic Th17 cell generation and autoimmune disease by fine-tuning redox homeostasis and mitochondrial integrity.
Journal: Proc Natl Acad Sci U S A | Authors: Wenhao Hu | Species: mouse | Application: WB - Rictor orchestrates β-catenin/FOXO balance by maintaining redox homeostasis during development of ovarian cancer
Journal: Oncogene | Authors: Xuejiao Zhao | Species: human | Application: WB - Protective effect of naringin against the LPS-induced apoptosis of PC12 cells: Implications for the treatment of neurodegenerative disorders.
Journal: Int J Mol Med | Authors: Hui Wang | Species: human | Application: WB - Contribution of ferroptosis and SLC7A11 to light-induced photoreceptor degeneration
Journal: Neural Regen Res | Authors: Xiaoxu Huang | Species: mouse | Application: WB - Distinct viral strategies of Porcine Epidemic Diarrhea Virus NSP1 and NSP2 converge on KEAP1 to degrade NRF2 and suppress host antioxidant defense.
Journal: Free Radic Biol Med | Authors: Jiacong Mo - Cystathionine γ-lyase deficiency exacerbates high-fat diet-induced kidney and liver injury associated with disrupted glutathione homeostasis.
Journal: Free Radic Biol Med | Authors: Gibong Jang - Melatonin-induced ferroptosis in pancreatic cancer cells by stimulating endoplasmic reticulum stress and inhibiting alanine-serine-cysteine transporter 2-driven glutamine metabolism.
Journal: World J Gastroenterol | Authors: Qian Zhao | Species: human | Application: WB,IF - Pyroptotic macrophages induce disruption of glutamate metabolism in periodontal ligament stem cells contributing to their compromised osteogenic potential.
Journal: Cell Prolif | Authors: Li-Juan Sun | Species: human | Application: WB - Effects of solamargine in hepatic metastasis of colorectal cancer: induction of ferroptosis and elimination of cancer stem cells
Journal: Chin Med | Authors: Shenglan Liu | Species: human | Application: WB,IHC - IMCA Induces Ferroptosis Mediated by SLC7A11 through the AMPK/mTOR Pathway in Colorectal Cancer.
Journal: Oxid Med Cell Longev | Authors: Lei Zhang | Species: human | Application: WB - The Role of SLC7A11 in Arsenite-Induced Oncogenic Phenotypes of Human Bronchial Epithelial Cells: A Metabolic Perspective
Journal: Environ Pollut | Authors: Xin Fang | Species: human | Application: WB - Fuxin decoction regulates the Nrf2/SLC7A11/GPX4 pathway to ameliorate doxorubicin-induced ferroptosis in cardiomyocytes
Journal: J Ethnopharmacol | Authors: Quancheng Han | Application: WB - Cystathionine γ-Lyase Protects Against Choline-Deficient High-Fat Diet-Induced Metabolic Dysfunction-Associated Steatotic Liver Disease Through the Cysteine-Glutathione Axis in Mice.
Journal: Antioxid Redox Signal | Authors: Min Ji Kim | Species: mouse | Application: WB - Exogenous hydrogen sulphide alleviates diabetes-associated cognitive decline by inhibiting ferroptosis through Nrf2/GPX4 signalling pathway.
Journal: Life Sci | Authors: Wei Hua - Overexpression of glutathione synthetase gene improving redox homeostasis and chicken infectious bursal disease virus propagation in chicken embryo fibroblast DF-1
Journal: Bioresour Bioprocess | Authors: Jia Lin | Species: chicken | Application: WB - Calycosin alleviates ferroptosis and attenuates doxorubicin-induced myocardial injury via the Nrf2/SLC7A11/GPX4 signaling pathway
Journal: Front Pharmacol | Authors: Quancheng Han | Species: rat | Application: WB - Salvia miltiorrhiza extract ameliorated ovarian damage induced by cyclophosphamide in mice by multidimensional mechanisms
Journal: J Ovarian Res | Authors: Lin Zhou | Species: mouse | Application: IHC - Effect of DJ-1 on the neuroprotection of astrocytes subjected to cerebral ischemia/reperfusion injury.
Journal: J Mol Med (Berl) | Authors: Li Peng | Species: rat | Application: WB - Potent effects of dioscin against obesity in mice.
Journal: Sci Rep | Authors: Min Liu | Species: mouse | Application: WB - Large-sized polystyrene microplastics induce oxidative stress in AML12 cells.
Journal: Sci Rep | Authors: Bitian Zhao | Species: mouse | Application: WB - Exogenous H(2)S reduces oxidative stress induced by lipid mixture in HepG2 cells through USP22/SIRT1 axis.
Journal: Sci Rep | Authors: Xiaomeng Cui | Species: human | Application: WB - HADH suppresses clear cell renal cell carcinoma progression through reduced NRF2-dependent glutathione synthesis
Journal: Transl Oncol | Authors: Changbin Chu | Species: human | Application: WB - Biochanin A Exerts Broad-Spectrum Antiviral Activity Against Coronaviruses via Activating the AMPK/Nrf2/GSH Pathway.
Journal: Microorganisms | Authors: Qisheng Lin | Species: monkey | Application: WB - Taurine treatment prevents derangement of the hepatic γ-glutamyl cycle and methylglyoxal metabolism in a mouse model of classical homocystinuria: regulatory crosstalk between thiol and sulfinic acid metabolism.
Journal: FASEB J | Authors: Kenneth N Maclean | Species: mouse | Application: WB - Pathological and biological significance of the specific glycan, TRA-1-60, on aggressive gastric adenocarcinoma
Journal: Lab Invest | Authors: Ayaka Mitsui | Species: human | Application: IF - l-Methionine activates Nrf2-ARE pathway to induce endogenous antioxidant activity for depressing ROS-derived oxidative stress in growing rats.
Journal: J Sci Food Agric | Authors: Zhengxuan Wang | Species: rat | Application: WB - Diarylpropionitrile, a selective agonist of estrogen receptor β, protects the kidney against ischemia-reperfusion injury and enhances the repair of tubular epithelial cells.
Journal: Naunyn Schmiedebergs Arch Pharmacol | Authors: Yong Kwon Han | Species: mouse | Application: WB - Exposure of Rats to Multiple Oral Doses of Dichloroacetate Results in Upregulation of Hepatic Glutathione Transferases and NAD(P)H Dehydrogenase [Quinone] 1.
Journal: Drug Metab Dispos | Authors: Edwin J Squirewell | Species: rat | Application: WB - STAT3 S-glutathionylation regulates Th17 differentiation in experimental immune thrombocytopenia.
Journal: Exp Cell Res | Authors: Gulibaha Maimaiti - Glutamate rescues heat stress-induced apoptosis of Sertoli cells by enhancing the activity of antioxidant enzymes and activating the Trx1-Akt pathway in vitro
Journal: Theriogenology | Authors: Xia-Qing Cai | Species: pig | Application: WB - Combination with vorinostat enhances the antitumor activity of cisplatin in castration-resistant prostate cancer by inhibiting DNA damage repair pathway and detoxification of GSH
Journal: Prostate | Authors: Taihao Chang | Species: human | Application: WB - Effect of Type I Diabetes on the Proteome of Mouse Oocytes.
Journal: Cell Physiol Biochem | Authors: Guangjian Jiang | Species: mouse | Application: WB - NCF-1 plays a pivotal role in the survival of adenocarcinoma cells of pancreatic and gastric origins
Journal: In Vitro Cell Dev Biol Anim | Authors: Chiemi Furuya-Ikude | Species: human | Application: IHC,IF - Capsaicin acts as a novel NRF2 agonist to suppress ethanol induced gastric mucosa oxidative damage by directly disrupting the KEAP1-NRF2 interaction.
Journal: Elife | Authors: Xiaoning Gao | Species: human | Application: WB
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