USP28 Polyclonal antibody 17707-1-AP proteintech
$149.00
In stock
SKU
17707-1-AP
| Catalog Number | 17707-1-AP |
| Synonyms | Deubiquitinating enzyme 28, EC:3.4.19.12, KIAA1515, Ubiquitin carboxyl-terminal hydrolase 28, Ubiquitin thiolesterase 28 |
| Host | Rabbit |
| Reactivity | human, mouse, rat |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, IHC, IF, IP, CoIP, ELISA |
| Applications | WB, IP, ELISA |
| Host / Isotype | Rabbit / IgG |
| Tested Applications — Positive WB detected in | A431 cells, HeLa cells, mouse heart tissue, MDA-MB-231 cells |
| Tested Applications — Positive IP detected in | HeLa cells |
| Recommended Dilutions — Western Blot (WB) | WB : 1:2000-1:16000 |
| Recommended Dilutions — Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Positive WB detected in | A431 cells, HeLa cells, mouse heart tissue, MDA-MB-231 cells |
| Positive IP detected in | HeLa cells |
| Western Blot (WB) | WB : 1:2000-1:16000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CatNo: Ag12003 Product name: Recombinant human USP28 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-350 aa of BC065928 Sequence: MTAELQQDDAAGAADGHGSSCQMLLNQLREITGIQDPSFLHEALKASNGDITQAVSLLTDERVKEPSQDTVATEPSEVEGSAANKEVLAKVIDLTHDNKDDLQAAIALSLLESPKIQADGRDLNRMHEATSAETKRSKRKRCEVWGENPNPNDWRRVDGWPVGLKNVGNTCWFSAVIQSLFQLPEFRRLVLSYSLPQNVLENCRSHTEKRNIMFMQELQYLFALMMGSNRKFVDPSAALDLLKGAFRSSEEQQQDVSEFTHKLLDWLEDAFQLAVNVNSPRNKSENPMVQLFYGTFLTEGVREGKPFCNNETFGQYPLQVNGYRNLDECLEGAMVEGDVELLPSDHSVKY Predict reactive species |
| Full Name | ubiquitin specific peptidase 28 |
| Calculated Molecular Weight | 583aa,66 kDa; 1077aa,120 kDa |
| Observed Molecular Weight | 135 kDa |
| GenBank Accession Number | BC065928 |
| Gene Symbol | USP28 |
| Gene ID (NCBI) | 57646 |
| RRID | AB_2272676 |
| Conjugate | Unconjugated |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q96RU2 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| IP protocol for USP28 antibody 17707-1-AP | Download protocol |
| WB protocol for USP28 antibody 17707-1-AP | Download protocol |
| mouse | WB,IHC J Clin Invest The deubiquitinase USP28 controls intestinal homeostasis and promotes colorectal cancer. Authors - Markus E Diefenbacher KD Validated View Article |
| human | IF J Cell Biol A USP28-53BP1-p53-p21 signaling axis arrests growth after centrosome loss or prolonged mitosis. Authors - Bramwell G Lambrus View Article |
| mouse,human | WB,IHC J Biol Chem The Deubiquitinase USP28 Maintains the Expression of the Transcription Factor MYCN and Is Essential in Neuroblastoma Cells Authors - Junjun Li KD Validated View Article |
| Nikhil (Verified Customer) (11-10-2019) | Detect endogenous USP28 in A549 cell line Applications: Western Blot, Primary Antibody Dilution: 1:1500 Cell Tissue Type: A549 USP28 Antibody Western Blot, validation (1:1500 dilution) in A549 (Cat no:17707-1-AP) |
| Citations | 33 |
| Dilutions | WB : 1:2000-1:16000 IP : 0.5-4.0 ug for IP and 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate for WB |
| Isotype | IgG |
| Clonality | Polyclonal |
| Clone Number | - |
| Conjugation | Unconjugated |
USP28(Ubiquitin carboxyl-terminal hydrolase 28) is also named as KIAA1515. USP28 is a 53BP1-interacting protein and a putative deubiquitinating enzyme, and belongs to a family of enzymes, called deubiquitinating enzymes (DUBs), which remove ubiquitin from the ubiquitin conjugates(PMID:16901786). It can be phosphorylated upon DNA damage at Ser-67 and Ser-714, by ATM or ATR(PMID:16901786). This protein has some isoforms produced by alternative splicing with the molecular mass of 122 kDa, 119 kDa and 66 kDa, and can be detected as 130-140 kDa (PMID: 16901786). Protocols Product Specific Protocols IP protocol for USP28 antibody 17707-1-AP Download protocol WB protocol for USP28 antibody 17707-1-AP Download protocol Standard Protocols Click here to view our Standard Protocols
Publications
- The deubiquitinase USP28 controls intestinal homeostasis and promotes colorectal cancer.
Journal: J Clin Invest | Authors: Markus E Diefenbacher KD Validated | Species: mouse | Application: WB,IHC - Prostate-specific oncogene OTUD6A promotes prostatic tumorigenesis via deubiquitinating and stabilizing c-Myc.
Journal: Cell Death Differ | Authors: Yunhua Peng KO Validated | Species: human | Application: WB - ANKRD26 recruits PIDD1 to centriolar distal appendages to activate the PIDDosome following centrosome amplification.
Journal: EMBO J | Authors: Lauren T Evans | Species: human | Application: IF - The Deubiquitinase USP28 Stabilizes LSD1 and Confers Stem-Cell-like Traits to Breast Cancer Cells.
Journal: Cell Rep | Authors: Wu Yadi Y | Species: human | Application: IHC - A USP28-53BP1-p53-p21 signaling axis arrests growth after centrosome loss or prolonged mitosis.
Journal: J Cell Biol | Authors: Bramwell G Lambrus | Species: human | Application: IF - The Deubiquitinase USP28 Maintains the Expression of the Transcription Factor MYCN and Is Essential in Neuroblastoma Cells
Journal: J Biol Chem | Authors: Junjun Li KD Validated | Species: mouse,human | Application: WB,IHC - Prostate-specific oncogene OTUD6A promotes prostatic tumorigenesis via deubiquitinating and stabilizing c-Myc.
Journal: Cell Death Differ | Authors: Yunhua Peng | Species: human | Application: WB - The deubiquitinase USP24 suppresses ferroptosis in triple-negative breast cancer by stabilizing DHODH protein
Journal: Cell Death Dis | Authors: Li Yang | Species: human | Application: WB - USP28 promotes PARP inhibitor resistance by enhancing SOX9-mediated DNA damage repair in ovarian cancer
Journal: Cell Death Dis | Authors: Fang Han | Species: human,mouse | Application: WB,IHC,CoIP - Regulation of Bax-dependent apoptosis by mitochondrial deubiquitinase USP30.
Journal: Cell Death Discov | Authors: Ding Yan | Species: human | Application: WB - FBXW7-mediated CHK2 regulation modulates DNA damage response and cellular stability in Huntington's disease.
Journal: Cell Death Discov | Authors: Tae Eun Kang | Species: mouse | Application: WB - ANKRD26 recruits PIDD1 to centriolar distal appendages to activate the PIDDosome following centrosome amplification.
Journal: EMBO J | Authors: Lauren T Evans | Species: human | Application: IF - The Deubiquitinase USP28 Stabilizes LSD1 and Confers Stem-Cell-like Traits to Breast Cancer Cells.
Journal: Cell Rep | Authors: Wu Yadi Y | Species: human | Application: IHC - USP28 promotes tumorigenesis and cisplatin resistance by deubiquitinating MAST1 protein in cancer cells
Journal: Cell Mol Life Sci | Authors: Janardhan Keshav Karapurkar | Species: human | Application: WB,IP,IHC,IF,CoIP - Inhibition of deubiquitinase USP28 attenuates cyst growth in autosomal dominant polycystic kidney disease
Journal: Biochem Pharmacol | Authors: Ying Ren | Species: mouse | Application: WB,IF - USP28-mediated deubiquitination of FOXK1 activates the Hippo signaling pathway to regulate cell proliferation and radiosensitivity in lung cancer
Journal: Life Sci | Authors: Yimei Meng | Species: human | Application: WB - A USP28-53BP1-p53-p21 signaling axis arrests growth after centrosome loss or prolonged mitosis.
Journal: J Cell Biol | Authors: Bramwell G Lambrus | Species: human | Application: IF - USP28 promotes tumor progression and glycolysis by stabilizing PKM2/Hif1-α in cholangiocarcinoma
Journal: Cell Oncol (Dordr) | Authors: Qian Qiao | Species: mouse,human | Application: WB,IHC,IF,CoIP - The stability and oncogenic function of LIN28A are regulated by USP28.
Journal: Biochim Biophys Acta Mol Basis Dis | Authors: Saba Haq | Species: human | Application: WB - The deubiquitinase USP28 promotes esophageal squamous cell carcinoma proliferation by stabilizing ΔNp63 protein.
Journal: Cell Signal | Authors: Changzhou Cai | Species: human | Application: WB - Targeting USP28 inhibits clear cell renal cell carcinoma growth.
Journal: Cell Signal | Authors: Ying Ren | Species: human | Application: WB,IP,IF - A nonautophagic role of ATG5 in regulating cell growth by targeting c-Myc for proteasome-mediated degradation.
Journal: iScience | Authors: Sheng Li | Species: human | Application: WB - The Deubiquitinase USP28 Maintains the Expression of the Transcription Factor MYCN and Is Essential in Neuroblastoma Cells
Journal: J Biol Chem | Authors: Junjun Li | Species: mouse,human | Application: WB,IHC - The Deubiquitinase USP28 Stabilizes Expression of RecQ Family Helicases and Maintains the Viability of Triple Negative Breast Cancer Cells.
Journal: J Biol Chem | Authors: Jiewei Wang | Species: human | Application: WB,IP - Mitochondria-targeted Catalase induced cell malignant transformation by the downregulation of p53 protein stability via USP28/miR-200b/PP2A-Cα axis
Journal: Arch Biochem Biophys | Authors: Chaowei Wen | Species: human | Application: WB - Blocking the interaction between circTNRC18 and LIN28A promotes trophoblast epithelial-mesenchymal transformation and alleviates preeclampsia
Journal: Mol Cell Endocrinol | Authors: Li-Li Chen | Species: human | Application: WB - USP28 Promotes Osimertinib Resistance in H1975 NSCLC Cells by Deubiquitinating and Stabilizing SIRT1.
Journal: Kaohsiung J Med Sci | Authors: Hu‐Sen Fan | Species: human | Application: WB,IP,CoIP - Loss of polycystins suppresses deciliation via the activation of the centrosomal integrity pathway.
Journal: Life Sci Alliance | Authors: Vasileios Gerakopoulos | Species: mouse | Application: WB,IF - Ubiquitin-specific peptidase 28 enhances STAT3 signaling and promotes cell growth in non-small-cell lung cancer.
Journal: Onco Targets Ther | Authors: Pengling Li | Species: human | Application: WB - Inhibition of Pard3 promotes breast cancer metastasis via the USP28 mediated deubiquitination of Snail1
Journal: Heliyon | Authors: Zhengyi Liu | Species: human | Application: WB - GMCL1 controls 53BP1 stability and modulates taxane sensitivity.
Journal: Elife | Authors: Yuki Kito | Species: human | Application: WB - Inhibition of Pard3 promotes breast cancer metastasis via the USP28 mediated deubiquitination of Snail1
Journal: Heliyon | Authors: Zhengyi Liu | Species: human | Application: WB - Comprehensive prognostic and immunological analysis of Ubiquitin Specific Peptidase 28 in pan-cancers and identification of its role in hepatocellular carcinoma cell lines
Journal: Aging (Albany NY) | Authors: Wuhan Zhou | Species: human | Application: WB,IHC,IF - A USP28-BRD4 axis contributes to the acceleration of breast cancer growth by hyperglycemia.
Journal: Cell Rep | Authors: Neng Li | Species: human, mouse | Application: WB,IP,CoIP
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