NDRG1 Polyclonal antibody 26902-1-AP proteintech
$149.00
In stock
SKU
26902-1-AP
| Catalog Number | 26902-1-AP |
| Synonyms | CMT4D, Differentiation-related gene 1 protein, DRG1, DRG-1, Protein NDRG1 |
| Host | Rabbit |
| Reactivity | human and More (1) |
| Reactivity | human, mouse |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA |
| Applications | WB, IHC, IF/ICC, FC (Intra), IP, ELISA |
| Host / Isotype | Rabbit / IgG |
| Tested Applications — Positive WB detected in | HEK-293T cells, HEK-293 cells, HeLa cells, Jurkat cells, mouse kidney tissue |
| Tested Applications — Positive IP detected in | HEK-293 cells |
| Tested Applications — Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Tested Applications — Positive IF/ICC detected in | HeLa cells |
| Tested Applications — Positive FC (Intra) detected in | HeLa cells |
| Recommended Dilutions — Western Blot (WB) | WB : 1:500-1:2000 |
| Recommended Dilutions — Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Recommended Dilutions — Immunohistochemistry (IHC) | IHC : 1:400-1:1600 |
| Recommended Dilutions — Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Recommended Dilutions — Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| Positive WB detected in | HEK-293T cells, HEK-293 cells, HeLa cells, Jurkat cells, mouse kidney tissue |
| Positive IP detected in | HEK-293 cells |
| Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
| Positive FC (Intra) detected in | HeLa cells |
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:400-1:1600 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CatNo: Ag25424 Product name: Recombinant human NDRG1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 45-109 aa of BC006260 Sequence: SGSSVTSLDGTRSRSHTSEGTRSRSHTSEGTRSRSHTSEGAHLDITPNSGAAGNSAGPKSMEVSC Predict reactive species |
| Full Name | N-myc downstream regulated 1 |
| Calculated Molecular Weight | 43 kDa |
| Observed Molecular Weight | 43 kDa |
| GenBank Accession Number | BC006260 |
| Gene Symbol | NDRG1 |
| Gene ID (NCBI) | 10397 |
| RRID | AB_2880676 |
| Conjugate | Unconjugated |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q92597 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| FC protocol for NDRG1 antibody 26902-1-AP | Download protocol |
| IF protocol for NDRG1 antibody 26902-1-AP | Download protocol |
| IHC protocol for NDRG1 antibody 26902-1-AP | Download protocol |
| IP protocol for NDRG1 antibody 26902-1-AP | Download protocol |
| WB protocol for NDRG1 antibody 26902-1-AP | Download protocol |
| mouse,human | IHC,IF Nat Aging Single-cell and spatial RNA sequencing identify divergent microenvironments and progression signatures in early- versus late-onset prostate cancer Authors - Yifei Cheng View Article |
| human | Mol Biol Rep Excessive iodine induces thyroid follicular epithelial cells apoptosis by activating HIF-1α-mediated hypoxia pathway in Hashimoto thyroiditis Authors - Lili Zhang View Article |
| Citations | 35 |
| Dilutions | WB : 1:500-1:2000 IP : 0.5-4.0 ug for IP and 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate for WB IHC : 1:400-1:1600 IF/ICC : 1:50-1:500 FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| Isotype | IgG |
| Clonality | Polyclonal |
| Clone Number | - |
| Conjugation | Unconjugated |
NDRG1 is a member of the N-myc down-regulated gene family which belongs to the alpha/beta hydrolase superfamily. NDRG1 is a highly conserved cytoplasmic protein involved in cell growth arrest and proliferation, cell differentiation, DNA damage response, heavy metal response, the hypoxia response, tumorigenesis, and metastasis. NDRG1 is a potent metastasis suppressor in multiple tumor-types. NDRG1 appears to serve as a putative tumor suppressor and is down-regulated in colorectal, gastric, cervical, ovarian and prostate cancers. Protocols Product Specific Protocols FC protocol for NDRG1 antibody 26902-1-AP Download protocol IF protocol for NDRG1 antibody 26902-1-AP Download protocol IHC protocol for NDRG1 antibody 26902-1-AP Download protocol IP protocol for NDRG1 antibody 26902-1-AP Download protocol WB protocol for NDRG1 antibody 26902-1-AP Download protocol Standard Protocols Click here to view our Standard Protocols
Publications
- Single-cell and spatial RNA sequencing identify divergent microenvironments and progression signatures in early- versus late-onset prostate cancer
Journal: Nat Aging | Authors: Yifei Cheng | Species: mouse,human | Application: IHC,IF - mTORC1 stimulates cell growth through SAM synthesis and m6A mRNA-dependent control of protein synthesis.
Journal: Mol Cell | Authors: Elodie Villa | Species: human | Application: WB - TARG1 protects against toxic DNA ADP-ribosylation.
Journal: Nucleic Acids Res | Authors: Callum Tromans-Coia KD Validated | Species: human | Application: WB - LINC01419 promotes cell proliferation and metastasis in hepatocellular carcinoma by enhancing NDRG1 promoter activity.
Journal: Cell Oncol (Dordr) | Authors: Hao Dang | Species: human | Application: WB,IHC - Deferasirox shows inhibition activity against cervical cancer in vitro and in vivo.
Journal: Gynecol Oncol | Authors: Nan Zhou | Species: human | Application: WB - Excessive iodine induces thyroid follicular epithelial cells apoptosis by activating HIF-1α-mediated hypoxia pathway in Hashimoto thyroiditis
Journal: Mol Biol Rep | Authors: Lili Zhang | Species: human
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