MLPH Polyclonal antibody 10338-1-AP proteintech
$149.00
In stock
SKU
10338-1-AP
| Catalog Number | 10338-1-AP |
| Synonyms | Exophilin 3, Exophilin-3, Melanophilin, SLAC2 A, SLAC2A |
| Host | Rabbit |
| Reactivity | human and More (2) |
| Reactivity | human, mouse, rat |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, IHC, IF/ICC, IP, ELISA |
| Host / Isotype | Rabbit / IgG |
| Tested Applications — Positive WB detected in | A375 cells, A549 cells, LNCaP cells, MCF-7 cells |
| Tested Applications — Positive IP detected in | A375 cells |
| Tested Applications — Positive IHC detected in | human skin cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Tested Applications — Positive IF/ICC detected in | MCF-7 cells |
| Recommended Dilutions — Western Blot (WB) | WB : 1:5000-1:50000 |
| Recommended Dilutions — Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Recommended Dilutions — Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Recommended Dilutions — Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| Positive WB detected in | A375 cells, A549 cells, LNCaP cells, MCF-7 cells |
| Positive IP detected in | A375 cells |
| Positive IHC detected in | human skin cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | MCF-7 cells |
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| Tested Reactivity | human |
| Cited Reactivity | human, mouse, rat |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CatNo: Ag0316 Product name: Recombinant human MLPH protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 8-236 aa of BC001653 Sequence: SKLTDEEAQHVLEVVQRDFDLRRKEEERLEALKGKIKKESSKRELLSDTAHLNETHCARCLQPYQLLVNSKRQCLECGLFTCKSCGRVHPEEQGWICDPCHLARVVKIGSLEWYYEHVKARFKRFGSAKVIRSLHGRLQGGAGPELISEERSGDSDQTDEDGEPGSEAQAQAQPFGSKKKRLLSVHDFDFEGDSDDSTQPQGHSLHLSSVPEARDSPQSLTDESCSEKA Predict reactive species |
| Full Name | melanophilin |
| Calculated Molecular Weight | 66 kDa |
| Observed Molecular Weight | 66 kDa, 80 kDa |
| GenBank Accession Number | BC001653 |
| Gene Symbol | MLPH |
| Gene ID (NCBI) | 79083 |
| RRID | AB_2146104 |
| Conjugate | Unconjugated |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9BV36 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| IF protocol for MLPH antibody 10338-1-AP | Download protocol |
| IHC protocol for MLPH antibody 10338-1-AP | Download protocol |
| IP protocol for MLPH antibody 10338-1-AP | Download protocol |
| WB protocol for MLPH antibody 10338-1-AP | Download protocol |
| human | WB J Invest Dermatol Knockdown of myosin Va isoforms by RNAi as a tool to block melanosome transport in primary human melanocytes. Authors - Van Gele Mireille M View Article |
| mouse | WB,IF Part Fibre Toxicol Coal dust exposure triggers heterogeneity of transcriptional profiles in mouse pneumoconiosis and Vitamin D remedies. Authors - Min Mu View Article |
| Ali (Verified Customer) (08-08-2025) | VERY GOOD SIGNAL Applications: Western Blot, Immunohistochemistry Cell Tissue Type: Skin |
| Citations | 32 |
| Dilutions | WB : 1:5000-1:50000 IP : 0.5-4.0 ug for IP and 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate for WB IHC : 1:50-1:500 IF/ICC : 1:200-1:800 |
| Isotype | IgG |
| Clonality | Polyclonal |
| Clone Number | - |
| Conjugation | Unconjugated |
Melanophilin (Mlph) is a member of the exophilin subfamily of Rab effector proteins. MLPH is a modular protein, forming a ternary complex with the small Ras-related GTPase Rab27A in its GTP-bound form and the motor protein myosin Va. It regulates retention of melanosomes at the peripheral actin cytoskeleton of melanocytes, a process essential for normal mammalian pigmentation. Defects in MLPH are a cause of Griscelli syndrome type 3 (GS3), which is characterized by a silver-gray hair color and abnormal pigment distribution in the hair shaft. Protocols Product Specific Protocols IF protocol for MLPH antibody 10338-1-AP Download protocol IHC protocol for MLPH antibody 10338-1-AP Download protocol IP protocol for MLPH antibody 10338-1-AP Download protocol WB protocol for MLPH antibody 10338-1-AP Download protocol Standard Protocols Click here to view our Standard Protocols
Publications
- Patients with Griscelli syndrome and normal pigmentation identify RAB27A mutations that selectively disrupt MUNC13-4 binding.
Journal: J Allergy Clin Immunol | Authors: Valentina Cetica | Species: human | Application: WB,IP - A novel function of Prohibitin on melanosome transport in melanocytes.
Journal: Theranostics | Authors: Chan Song Jo KD Validated | Species: human | Application: WB - O-GlcNAcylation of melanophilin enhances radiation resistance in glioblastoma via suppressing TRIM21 mediated ubiquitination
Journal: Oncogene | Authors: Lei Xu | Species: human | Application: WB,IP,IF - Functional hierarchy among different Rab27 effectors involved in secretory granule exocytosis
Journal: Elife | Authors: Kunli Zhao | Species: mouse | Application: WB - Coal dust exposure triggers heterogeneity of transcriptional profiles in mouse pneumoconiosis and Vitamin D remedies.
Journal: Part Fibre Toxicol | Authors: Min Mu | Species: mouse | Application: WB,IF - Knockdown of myosin Va isoforms by RNAi as a tool to block melanosome transport in primary human melanocytes.
Journal: J Invest Dermatol | Authors: Van Gele Mireille M | Species: human | Application: WB - Autophagy related 7 dysfunction in senescent melanocytes and hypopigmented skin: reversal by metformin.
Journal: Br J Dermatol | Authors: Jin Cheol Kim | Species: human | Application: WB - Knockdown of myosin Va isoforms by RNAi as a tool to block melanosome transport in primary human melanocytes.
Journal: J Invest Dermatol | Authors: Van Gele Mireille M | Species: human | Application: WB - Anti-Hyperpigmentation-Related Potential Activities in B16BL6 Cells and Chemical Composition of Essential Oil from Chamaecyparis pisifera Leaves.
Journal: Pharmaceutics | Authors: Do Yoon Kim | Species: mouse | Application: WB - MLPH/RAB3A accelerates the differentiation of pancreatic stem cells to islet β-cells to control blood glucose in diabetic rats.
Journal: Organogenesis | Authors: Junling Shan | Species: rat | Application: WB,IHC - Phytochemical Composition and In Vitro Anti-Pigmentation Activity of Persicaria senticosa Flower Absolute: Potential Dual Inhibition of Melanogenesis and Melanosome Transport.
Journal: Pharmaceuticals (Basel) | Authors: Kyung Jong Won | Species: mouse | Application: WB - Differential gene expression in normal esophagus and Barrett's esophagus.
Journal: J Gastroenterol | Authors: Jacob Wang | Species: human | Application: IHC - Identification of MicroRNA Targeting Mlph and Affecting Melanosome Transport.
Journal: Biomolecules | Authors: Jeong Ah Lee | Species: human | Application: WB - Acetylation-enhanced Sp1 transcriptional activity suppresses Mlph expression
Journal: Sci Rep | Authors: Chan Song Jo | Species: Mouse | Application: WB - Regulation of Melanophilin (Mlph) gene expression by the glucocorticoid receptor (GR).
Journal: Sci Rep | Authors: Cheol Hwan Myung | Species: mouse | Application: WB - Oxyresveratrol suppressed melanogenesis, dendrite formation, and melanosome transport in melanocytes via regulation of the MC1R/cAMP/MITF pathway.
Journal: Sci Rep | Authors: Jianhua Zhang | Species: mouse | Application: WB - Inhibitory effect of 2-methyl-naphtho[1,2,3-de]quinolin-8-one on melanosome transport and skin pigmentation.
Journal: Sci Rep | Authors: Jong Il Park | Species: human | Application: WB - Metformin inhibits melanin synthesis and melanosome transfer through the cAMP pathway
Journal: Sci Rep | Authors: Xing Liu | Species: human | Application: WB - Chemical Composition and Skin-Whitening Activities of Siegesbeckia glabrescens Makino Flower Absolute in Melanocytes
Journal: Plants (Basel) | Authors: Da Kyoung Lee | Species: mouse | Application: WB - Transcriptional regulation of Mlph and Rab27a by PAX3-NF-κB interaction in melanosome transport of melanocytes.
Journal: J Dermatol Sci | Authors: HaiRu Zhao | Species: mouse | Application: WB - 16-Kauren-2-beta-18,19-triol inhibits melanosome transport in melanocytes by down-regulation of melanophilin expression.
Journal: J Dermatol Sci | Authors: Cheol Hwan Myung | Species: mouse | Application: WB - A novel missense mutation (G43S) in the switch I region of Rab27A causing Griscelli syndrome.
Journal: Mol Genet Metab | Authors: Westbroek Wendy W | Species: human | Application: WB - Identification of a Novel MLPH Missense Mutation in a Chinese Griscelli Syndrome 3 Patient.
Journal: Front Med (Lausanne) | Authors: Qiaorong Huang | Species: human | Application: WB - Cellular and clinical report of new Griscelli syndrome type III cases.
Journal: Pigment Cell Melanoma Res | Authors: Westbroek Wendy W | Species: human | Application: WB - Cellular and clinical report of new Griscelli syndrome type III cases.
Journal: Pigment Cell Melanoma Res | Authors: Westbroek Wendy W | Species: human | Application: WB - Low Molecular Weight Collagen Peptide (LMWCP) Promotes Hair Growth by Activating the Wnt/GSK-3β/β-Catenin Signaling Pathway
Journal: J Microbiol Biotechnol | Authors: Yujin Kim - Corrigendum to: Low Molecular Weight Collagen Peptide (LMWCP) Promotes Hair Growth by Activating the Wnt/GSK-3β/β-Catenin Signaling Pathway
Journal: J Microbiol Biotechnol | Authors: Yujin Kim - MLPH regulates EMT in pancreatic adenocarcinoma through the PI3K-AKT signaling pathway
Journal: J Cancer | Authors: Mengda Wei | Species: human | Application: WB - Phosphorylation of glucocorticoid receptor induced by 16-kauren-2-beta-18, 19-triol decreases expression of Melanophilin through JNK signalling
Journal: Exp Dermatol | Authors: Cheol-Hwan Myung | Species: mouse | Application: WB,IF - Depigmenting Effects of Vitex rotundifolia Cone Essential Oil in Melanocytes and Its Chemical Composition.
Journal: Chem Biodivers | Authors: Da Yeon Yoo | Species: mouse | Application: WB - MLPH Accelerates the Epithelial-Mesenchymal Transition in Prostate Cancer.
Journal: Onco Targets Ther | Authors: Tianbiao Zhang | Species: human | Application: WB - Functional hierarchy among different Rab27 effectors involved in secretory granule exocytosis
Journal: Elife | Authors: Kunli Zhao | Species: mouse | Application: WB
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