CAPG Polyclonal antibody 10194-1-AP proteintech
$149.00
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SKU
10194-1-AP
| Catalog Number | 10194-1-AP |
| Synonyms | Actin regulatory protein CAP-G, Macrophage-capping protein |
| Host | Rabbit |
| Reactivity | human, mouse, rat |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, IHC, IF/ICC, IP, ELISA |
| Applications | WB, IHC, IP, ELISA |
| Host / Isotype | Rabbit / IgG |
| Tested Applications — Positive WB detected in | HeLa cells, HL-60 cells, mouse heart tissue, mouse lung tissue, rat heart tissue, rat lung tissue |
| Tested Applications — Positive IP detected in | HeLa cells, mouse heart tissue |
| Tested Applications — Positive IHC detected in | human prostate cancer tissue, human tonsillitis tissue, human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Recommended Dilutions — Western Blot (WB) | WB : 1:5000-1:50000 |
| Recommended Dilutions — Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Recommended Dilutions — Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Positive WB detected in | HeLa cells, HL-60 cells, mouse heart tissue, mouse lung tissue, rat heart tissue, rat lung tissue |
| Positive IP detected in | HeLa cells, mouse heart tissue |
| Positive IHC detected in | human prostate cancer tissue, human tonsillitis tissue, human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CatNo: Ag0248 Product name: Recombinant human CAPG protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 63-348 aa of BC000728 Sequence: LWIGQQSSRDEQGACAVLAVHLNTLLGERPVQHREVQGNESDLFMSYFPRGLKYQEGGVESAFHKTSTGAPAAIKKLYQVKGKKNIRATERALNWDSFNTGDCFILDLGQNIFAWCGGKSNILERNKARDLALAIRDSERQGKAQVEIVTDGEEPAEMIQVLGPKPALKEGNPEEDLTADKANAQAAALYKVSDATGQMNLTKVADSSPFALELLISDDCFVLDNGLCGKIYIWKGRKANEKERQAALQVAEGFISRMQYAPNTQVEILPQGRESPIFKQFFKDWK Predict reactive species |
| Full Name | capping protein (actin filament), gelsolin-like |
| Calculated Molecular Weight | 39 kDa |
| Observed Molecular Weight | 39-42 kDa |
| GenBank Accession Number | BC000728 |
| Gene Symbol | CAPG |
| Gene ID (NCBI) | 822 |
| RRID | AB_2069179 |
| Conjugate | Unconjugated |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P40121 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| IHC protocol for CAPG antibody 10194-1-AP | Download protocol |
| IP protocol for CAPG antibody 10194-1-AP | Download protocol |
| WB protocol for CAPG antibody 10194-1-AP | Download protocol |
| mouse | WB Mol Cell Proteomics Quantitative protein and mRNA profiling shows selective post-transcriptional control of protein expression by vasopressin in kidney cells. Authors - Khositseth Sookkasem S View Article |
| human | WB Proteomics Proteomics-based identification of two novel direct targets of hypoxia-inducible factor-1 and their potential roles in migration/invasion of cancer cells. Authors - Liao Shi-Hua SH View Article |
| Citations | 28 |
| Dilutions | WB : 1:5000-1:50000 IP : 0.5-4.0 ug for IP and 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate for WB IHC : 1:50-1:500 |
| Isotype | IgG |
| Clonality | Polyclonal |
| Clone Number | - |
| Conjugation | Unconjugated |
CapG (gelsolin-like actin-capping protein) is a member of the gelsolin superfamily proteins, which regulates actin filament length by capping or severing filaments. Studies have shown a role for CapG in regulating cell motility. The expression of CapG is upregulated under hypoxia and in turn increases the motility of cells. Recently it has been reported that the overexpression of CapG is associated with the progression of lung adenocarcinoma as well as lyphatic invasion status of cholangiocarcinoma, which may be used for prognosis of these tumors.(21908955,22155129) Protocols Product Specific Protocols IHC protocol for CAPG antibody 10194-1-AP Download protocol IP protocol for CAPG antibody 10194-1-AP Download protocol WB protocol for CAPG antibody 10194-1-AP Download protocol Standard Protocols Click here to view our Standard Protocols
Publications
- Quantitative protein and mRNA profiling shows selective post-transcriptional control of protein expression by vasopressin in kidney cells.
Journal: Mol Cell Proteomics | Authors: Khositseth Sookkasem S | Species: mouse | Application: WB - Laser microdissection and two-dimensional difference gel electrophoresis reveal the role of a novel macrophage-capping protein in lymph node metastasis in gastric cancer.
Journal: J Proteome Res | Authors: Ichikawa Hiroshi H | Species: human | Application: WB - Differential proteomic analysis of synovial fluid from rheumatoid arthritis and osteoarthritis patients.
Journal: Clin Proteomics | Authors: Lavanya Balakrishnan | Species: human | Application: WB - PMID: 25586482
Journal: Inflammation | Authors: Xin Ma | Species: human | Application: WB - Up-regulation of two actin-associated proteins prompts pulmonary artery smooth muscle cell migration under hypoxia.
Journal: Am J Respir Cell Mol Biol | Authors: Zhang Ruifeng R KD Validated | Species: human | Application: WB - Proteomics-based identification of two novel direct targets of hypoxia-inducible factor-1 and their potential roles in migration/invasion of cancer cells.
Journal: Proteomics | Authors: Liao Shi-Hua SH | Species: human | Application: WB - Propofol Attenuates Lipopolysaccharide-Induced Monocyte Chemoattractant Protein-1 Production Through Enhancing apoM and foxa2 Expression in HepG2 Cells.
Journal: Inflammation | Authors: Xin Ma | Species: human | Application: WB - Tumor vascular disrupting agent 5,6-dimethylxanthenone-4-acetic acid (DMXAA): Suppresses macrophage capping protein beyond STING activation
Journal: Biochim Biophys Acta Mol Basis Dis | Authors: Zhiyong Xiao | Species: human | Application: WB - Exogenous extracellular matrix proteins decrease cardiac fibroblast activation in stiffening microenvironment through CAPG.
Journal: J Mol Cell Cardiol | Authors: Xinming Wang | Species: rat,mouse | Application: WB,IF - Differential proteomic analysis of synovial fluid from rheumatoid arthritis and osteoarthritis patients.
Journal: Clin Proteomics | Authors: Lavanya Balakrishnan | Species: human | Application: WB - Functional analysis of the CXCR1a gene response to SGIV viral infection in grouper.
Journal: Fish Shellfish Immunol | Authors: Qing Wang | Application: WB - Proteomics-based identification of two novel direct targets of hypoxia-inducible factor-1 and their potential roles in migration/invasion of cancer cells.
Journal: Proteomics | Authors: Liao Shi-Hua SH | Species: human | Application: WB - Laser microdissection and two-dimensional difference gel electrophoresis reveal the role of a novel macrophage-capping protein in lymph node metastasis in gastric cancer.
Journal: J Proteome Res | Authors: Ichikawa Hiroshi H | Species: human | Application: WB - CAPG Is Required for Ebola Virus Infection by Controlling Virus Egress from Infected Cells
Journal: Viruses | Authors: Hiroyuki Mori | Species: human | Application: WB,IF - Facile discovery of red blood cell deformation and compromised membrane/skeleton assembly in Prader-Willi syndrome
Journal: Front Med | Authors: Yashuang Yang | Species: human | Application: WB - Analysis of a Sardinian Multiplex Family with Autism Spectrum Disorder Points to Post-Synaptic Density Gene Variants and Identifies CAPG as a Functionally Relevant Candidate Gene.
Journal: J Clin Med | Authors: Elena Bacchelli | Species: human | Application: WB - CAPG enhances adipogenesis and inflammatory cytokine expression in adipocytes.
Journal: Adipocyte | Authors: Luyao Zhang | Species: mouse | Application: WB - Macrophage-capping protein as a tissue biomarker for prediction of response to gemcitabine treatment and prognosis in cholangiocarcinoma.
Journal: J Proteomics | Authors: Morofuji Noriaki N | Species: human | Application: WB,IHC - Proteomic identification of the macrophage-capping protein as a protein contributing to the malignant features of hepatocellular carcinoma.
Journal: J Proteomics | Authors: Kimura Kazuya K | Species: human | Application: WB - Capg enhances proliferation, adipogenesis, and inflammatory response in preadipocytes: insights from bioinformatics analysis and functional validation.
Journal: PeerJ | Authors: Luyao Zhang | Species: mouse | Application: WB - Analysis and verification of potential ferroptosis-related diagnostic markers in the early stage of metabolic dysfunction-associated steatohepatitis
Journal: Biochem Biophys Res Commun | Authors: Qianqian Li | Application: WB,IHC - MnSOD marks cord blood late outgrowth endothelial cells and accompanies robust resistance to oxidative stress.
Journal: Biochem Biophys Res Commun | Authors: Cai Hao H | Species: human | Application: WB - PTTG1 induces pancreatic cancer cell proliferation and promotes aerobic glycolysis by regulating c-myc
Journal: Open Life Sci | Authors: Yong Wang | Species: mouse,human | Application: WB - Effects of quercetin on the expression of MCP-1, MMP-9 and VEGF in rats with diabetic retinopathy.
Journal: Exp Ther Med | Authors: Bin Chen | Species: rat | Application: WB - Effects of quercetin on the expression of MCP-1, MMP-9 and VEGF in rats with diabetic retinopathy.
Journal: Exp Ther Med | Authors: Bin Chen | Species: rat | Application: WB - Function of the macrophage-capping protein in colorectal carcinoma.
Journal: Oncol Lett | Authors: Wei Wu | Species: human | Application: WB,IHC - Gelsolin-like actin-capping protein is associated with patient prognosis, cellular apoptosis and proliferation in prostate cancer.
Journal: Biomark Med | Authors: Tieqiu Li | Species: human | Application: WB - Acquisition of useful sero-diagnostic autoantibodies using the same patients'sera and tumor tissues.
Journal: Biomed Res | Authors: Makoto Kobayashi | Species: human | Application: WB,IHC - A pan-tissue multi-omics resource atlas of coordinated immune, metabolic, and fibrotic alterations in diet-induced MASH.
Journal: iScience | Authors: Huajing Yu
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