FAF2 Polyclonal antibody 16251-1-AP proteintech
$149.00
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SKU
16251-1-AP
| Catalog Number | 16251-1-AP |
| Synonyms | ETEA, FAF2, FAS associated factor 2, KIAA0887, Protein ETEA, UBXD8, UBXN3B |
| Host | Rabbit |
| Reactivity | human, mouse, rat and More (1) |
| Reactivity | human, mouse, rat, bovine |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, IHC, IF, IP, ELISA |
| Applications | WB, IP, IHC, ELISA |
| Host / Isotype | Rabbit / IgG |
| Tested Applications — Positive WB detected in | HEK-293 cells, HepG2 cells, HeLa cells, mouse brain tissue, rat brain tissue, K-562 cells, MCF-7 cells, SH-SY5Y cells, U2OS cells |
| Tested Applications — Positive IP detected in | mouse brain tissue |
| Tested Applications — Positive IHC detected in | human breast cancer tissue, mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Recommended Dilutions — Western Blot (WB) | WB : 1:1000-1:8000 |
| Recommended Dilutions — Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Recommended Dilutions — Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Positive WB detected in | HEK-293 cells, HepG2 cells, HeLa cells, mouse brain tissue, rat brain tissue, K-562 cells, MCF-7 cells, SH-SY5Y cells, U2OS cells |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | human breast cancer tissue, mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, bovine |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CatNo: Ag9236 Product name: Recombinant human FAF2 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 97-445 aa of BC014001 Sequence: YLIMLPFRFTYYTILDIFRFALRFIRPDPRSRVTDPVGDIVSFMHSFEEKYGRAHPVFYQGTYSQALNDAKRELRFLLVYLHGDDHQDSDEFCRNTLCAPEVISLINTRMLFWACSTNKPEGYRVSQALRENTYPFLAMIMLKDRRMTVVGRLEGLIQPDDLINQLTFIMDANQTYLVSERLEREERNQTQVLRQQQDEAYLASLRADQEKERKKREERERKRRKEEEVQQQKLAEERRRQNLQEEKERKLECLPPEPSPDDPESVKIIFKLPNDSRVERRFHFSQSLTVIHDFLFSLKESPEKFQIEANFPRRVLPCIPSEEWPNPPTLQEAGLSHTEVLFVQDLTDE Predict reactive species |
| Full Name | Fas associated factor family member 2 |
| Calculated Molecular Weight | 445 aa, 53 kDa |
| Observed Molecular Weight | 53 kDa |
| GenBank Accession Number | BC014001 |
| Gene Symbol | FAF2 |
| Gene ID (NCBI) | 23197 |
| RRID | AB_2262469 |
| Conjugate | Unconjugated |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q96CS3 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| IHC protocol for FAF2 antibody 16251-1-AP | Download protocol |
| IP protocol for FAF2 antibody 16251-1-AP | Download protocol |
| WB protocol for FAF2 antibody 16251-1-AP | Download protocol |
| human | WB J Biol Chem Characterization of the deubiquitinating activity of USP19 and its role in endoplasmic reticulum-associated degradation. Authors - Jin-Gu Lee View Article |
| Citations | 29 |
| Dilutions | WB : 1:1000-1:8000 IP : 0.5-4.0 ug for IP and 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate for WB IHC : 1:50-1:500 |
| Isotype | IgG |
| Clonality | Polyclonal |
| Clone Number | - |
| Conjugation | Unconjugated |
Protein ETEA (FAF2, or UBXD8) is a homolog to Fas-associated factor 1 (FAF1), which is involved in Fas-mediated apoptosis. ETEA protein directly interacts with and negatively regulates neurofibromin. ETEA contains both UBA and UBX domains and overexpression of ETEA downregulates neurofibromin in human cells. It may play a role in the translocation of terminally misfolded proteins from the endoplasmic reticulum lumen to the cytoplasm and their degradation by the proteasome. ETEA is highly expressed in peripheral blood of patients with atopic dermatitis (AD) compared to normal individuals, and may regulate the resistance to apoptosis that is observed in T cells and eosinophils of AD patients. Protocols Product Specific Protocols IHC protocol for FAF2 antibody 16251-1-AP Download protocol IP protocol for FAF2 antibody 16251-1-AP Download protocol WB protocol for FAF2 antibody 16251-1-AP Download protocol Standard Protocols Click here to view our Standard Protocols
Publications
- Ubiquitination of G3BP1 mediates stress granule disassembly in a context-specific manner.
Journal: Science | Authors: Youngdae Gwon | Species: human | Application: IP,IF - Organelle contact reorganization drives calcium-dependent autophagy under proteostatic stress.
Journal: Autophagy | Authors: Yu bin Ko | Species: human | Application: WB,IF - hnRNPA2B1 represses the disassembly of arsenite-induced stress granules and is essential for male fertility
Journal: Cell Rep | Authors: Xiaoli Wang | Species: human | Application: WB - USP13 antagonizes gp78 to maintain functionality of a chaperone in ER-associated degradation.
Journal: Elife | Authors: Yanfen Liu - Interaction mapping of endoplasmic reticulum ubiquitin ligases identifies modulators of innate immune signalling.
Journal: Elife | Authors: Emma J Fenech | Species: human | Application: WB - Characterization of the deubiquitinating activity of USP19 and its role in endoplasmic reticulum-associated degradation.
Journal: J Biol Chem | Authors: Jin-Gu Lee | Species: human | Application: WB
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