GAPDH Monoclonal antibody 60004-1-Ig proteintech
$189.00
In stock
SKU
60004-1-Ig
| Catalog Number | 60004-1-Ig |
| Synonyms | 1E6D9 |
| Host | Mouse |
| Reactivity | human, mouse, rat, pig, zebrafish, yeast and More (7) |
| Reactivity | human, mouse, rat, pig, zebrafish, yeast, chicken, goat, deer, whiteflies, tick, treeshrew, cynomorium songaricum |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA |
| Applications | WB, IHC, IF/ICC, FC (Intra), IP, ELISA |
| Host / Isotype | Mouse / IgG2b |
| Positive WB detected in | HeLa cells, HepG2 cells, ROS1728 cells, pig brain tissue, zebrafish tissue, whole yeast, whole Nematode tissue, soybean whole plant tissue, arabidopsis whole plant tissue, HEK-293 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells, 4T1 cells, C6 cells, PC-12 cells, C2C12 cells, SP2/0 cells, rat brain tissue, mouse brain tissue |
| Positive IP detected in | HeLa Cells |
| Positive IHC detected in | human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
| Positive FC (Intra) detected in | HeLa cells |
| Western Blot (WB) | WB : 1:50000-1:500000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:3000-1:16000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| Tested Reactivity | human, mouse, rat, pig, zebrafish, yeast |
| Cited Reactivity | human, rat, chicken, goat, yeast, deer, whiteflies, tick, treeshrew, cynomorium songaricum |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | CatNo: Ag0766 Product name: Recombinant human GAPDH protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-335 aa of BC004109 Sequence: MGKVKVGVNGFGRIGRLVTRAAFNSGKVDIVAINDPFIDLNYMVYMFQYDSTHGKFHGTVKAENGKLVINGNPITIFQERDPSKIKWGDAGAEYVVESTGVFTTMEKAGAHLQGGAKRVIISAPSADAPMFVMGVNHEKYDNSLKIISNASCTTNCLAPLAKVIHDNFGIVEGLMTTVHAITATQKTVDGPSGKLWRDGRGALQNIIPASTGAAKAVGKVIPELNGKLTGMAFRVPTANVSVVDLTCRLEKPAKYDDIKKVVKQASEGPLKGILGYTEHQVVSSDFNSDTHSSTFDAGAGIALNDHFVKLISWYDNEFGYSNRVVDLMAHMASKE Predict reactive species |
| Full Name | glyceraldehyde-3-phosphate dehydrogenase |
| Calculated Molecular Weight | 36 kDa |
| Observed Molecular Weight | 36 kDa |
| GenBank Accession Number | BC004109 |
| Gene Symbol | GAPDH |
| Gene ID (NCBI) | 2597 |
| RRID | AB_2107436 |
| Conjugate | Unconjugated |
| Purification Method | Protein A purification |
| UNIPROT ID | P04406 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| FC protocol for GAPDH antibody 60004-1-Ig | Download protocol |
| IF protocol for GAPDH antibody 60004-1-Ig | Download protocol |
| IHC protocol for GAPDH antibody 60004-1-Ig | Download protocol |
| WB protocol for GAPDH antibody 60004-1-Ig | Download protocol |
| mouse | WB Signal Transduct Target Ther The Agpat4/LPA axis in colorectal cancer cells regulates antitumor responses via p38/p65 signaling in macrophages. Authors - Dapeng Zhang View Article |
| human | WB Signal Transduct Target Ther AHR mediates the aflatoxin B1 toxicity associated with hepatocellular carcinoma. Authors - Qing Zhu View Article |
| Citations | 19364 |
| Dilutions | WB : 1:50000-1:500000 IP : 0.5-4.0 ug for IP and 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate for WB IHC : 1:3000-1:16000 IF/ICC : 1:400-1:1600 FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| Isotype | IgG2b |
| Clonality | Monoclonal |
| Clone Number | 1E6D9 |
| Conjugation | Unconjugated |
Background Information
GAPDH (glyceraldehyde-3-phosphate dehydrogenase) catalyzes a glycolytic phosphorylation step and is commonly used as a housekeeping control in Western blotting and RT-PCR. It is mainly associated with cytosolic and membrane regions, with minimal nuclear expression; reported additional roles include stress-related apoptosis signaling, ER-to-Golgi vesicle transport, and interactions with TPPP, SIAH1, CHP1, RILPL1/GOSPEL, and FKBP6 (PMIDs: 21895736, 12829261, 25859407, 11035021). For murine tissue samples, HRP-60004 or 10494-1-AP is preferred.
Publications
- The Agpat4/LPA axis in colorectal cancer cells regulates antitumor responses via p38/p65 signaling in macrophages.
Journal: Signal Transduct Target Ther | Authors: Dapeng Zhang | Species: mouse | Application: WB - Breast cancer-derived exosomes transmit lncRNA SNHG16 to induce CD73+γδ1 Treg cells.
Journal: Signal Transduct Target Ther | Authors: Chao Ni | Species: human | Application: WB - A CRISPR knockout negative screen reveals synergy between CDKs inhibitor and metformin in the treatment of human cancer in vitro and in vivo.
Journal: Signal Transduct Target Ther | Authors: Yarui Ma | Species: human | Application: WB - The effect of whey protein on viral infection and replication of SARS-CoV-2 and pangolin coronavirus in vitro.
Journal: Signal Transduct Target Ther | Authors: Huahao Fan | Species: human | Application: WB - MELK is an oncogenic kinase essential for metastasis, mitotic progression, and programmed death in lung carcinoma.
Journal: Signal Transduct Target Ther | Authors: Qin Tang | Species: human | Application: WB - AHR mediates the aflatoxin B1 toxicity associated with hepatocellular carcinoma.
Journal: Signal Transduct Target Ther | Authors: Qing Zhu | Species: human | Application: WB
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