CoraLite® Plus 647 Anti-Mouse CD45R (B220) (RA3-6B2) CL647-65139 proteintech
$139.00
In stock
SKU
CL647-65139
| Catalog Number | CL647-65139 |
| Synonyms | B220, CD45R, Cd45, Ptprc, EC:3.1.3.48 |
| Host | Rat |
| Reactivity | mouse |
| Form | Liquid |
| Formulation | PBS, Azide, BSA |
| Applications | IF/ICC, FC |
| Host / Isotype | Rat / IgG2a, kappa |
| Tested Applications — Positive IF/ICC detected in | mouse splenocytes |
| Tested Applications — Positive FC detected in | mouse splenocytes |
| Recommended Dilutions — Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| Positive IF/ICC detected in | mouse splenocytes |
| Positive FC detected in | mouse splenocytes |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| Flow Cytometry (FC) | FC : 0.25 ug per 10^6 cells in 100 μl suspension |
| Tested Reactivity | mouse |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | Abelson murine leukemia virus-induced pre-B tumor cells Predict reactive species |
| Full Name | protein tyrosine phosphatase, receptor type, C |
| GenBank Accession Number | BC028512 |
| Gene Symbol | CD45 |
| Gene ID (NCBI) | 19264 |
| RRID | AB_2883705 |
| Conjugate | CoraLite® Plus 647 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 654 nm / 674 nm |
| Excitation Laser | Red Laser (633 nm) |
| Purification Method | Affinity purification |
| UNIPROT ID | P06800 |
| Storage Buffer | PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
| FC protocol for CL Plus 647 CD45R (B220) antibody CL647-65139 | Download protocol |
| IF protocol for CL Plus 647 CD45R (B220) antibody CL647-65139 | Download protocol |
| Citations | - |
| Dilutions | IF/ICC : 1:200-1:800 |
| Isotype | IgG2a, kappa |
| Clonality | Monoclonal |
| Clone Number | RA3-6B2 |
| Conjugation | CoraLite® Plus 647 |
CD45, also known as protein tyrosine phosphatase, receptor type C, is a type I transmembrane protein expressed on the surface of all haematopoietic cells with the exception of erythrocytes and platelets (PMID: 3489673; 28615666). CD45 is a pan-haematopoietic cell marker and has been shown to be essential for T- and B-cell activation and signalling (PMID: 9429890; 16378097). CD45R (B220) is an isoform of CD45, expressed on murine B-lineage cells (except for plasma cells) and on a small sub-population of T cells (PMID: 6975314; 15712182). It is commonly used as a pan B-cell marker in mice. CD45R is also expressed on activated T cells and NK cell subsets (PMID: 7514808; 17923504). The RA3-6B2 clone reacts with human and mouse CD45R. Protocols Product Specific Protocols FC protocol for CL Plus 647 CD45R (B220) antibody CL647-65139 Download protocol IF protocol for CL Plus 647 CD45R (B220) antibody CL647-65139 Download protocol Standard Protocols Click here to view our Standard Protocols
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