HIF-1 alpha Monoclonal antibody 66730-1-Ig proteintech
$149.00
In stock
SKU
66730-1-Ig
| Catalog Number | 66730-1-Ig |
| Synonyms | HIF1A, 1H3C12, ARNT interacting protein, ARNT-interacting protein, Basic-helix-loop-helix-PAS protein MOP1 |
| Host | Mouse |
| Reactivity | human |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, IHC, IF, IP, CoIP, ChIP, ELISA |
| Applications | WB, IHC, ELISA |
| Host / Isotype | Mouse / IgG1 |
| Tested Applications — Positive WB detected in | HeLa cells, Cobalt Chloride treated HeLa cells, Cobalt Chloride treated HepG2 cells |
| Tested Applications — Positive IHC detected in | human lung cancer tissue, human ovary tumor tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Recommended Dilutions — Western Blot (WB) | WB : 1:2000-1:10000 |
| Recommended Dilutions — Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Positive WB detected in | HeLa cells, Cobalt Chloride treated HeLa cells, Cobalt Chloride treated HepG2 cells |
| Positive IHC detected in | human lung cancer tissue, human ovary tumor tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Tested Reactivity | human |
| Cited Reactivity | human |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | CatNo: Ag15198 Product name: Recombinant human HIF1a protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 574-799 aa of BC012527 Sequence: LRSFDQLSPLESSSASPESASPQSTVTVFQQTQIQEPTANATTTTATTDELKTVTKDRMEDIKILIASPSPTHIHKETTSATSSPYRDTQSRTASPNRAGKGVIEQTEKSHPRSPNVLSVALSQRTTVPEEELNPKILALQNAQRKRKMEHDGSLFQAVGIGTLLQQPDDHAATTSLSWKRVKGCKSSEQNGMEQKTIILIPSDLACRLLGQSMDESGLPQLTSYD Predict reactive species |
| Full Name | hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor) |
| Calculated Molecular Weight | 826 aa, 93 kDa |
| Observed Molecular Weight | 120 kDa |
| GenBank Accession Number | BC012527 |
| Gene Symbol | HIF1A |
| Gene ID (NCBI) | 3091 |
| RRID | AB_2882080 |
| Conjugate | Unconjugated |
| Purification Method | Protein G purification |
| UNIPROT ID | Q16665 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| IHC protocol for HIF-1 alpha antibody 66730-1-Ig | Download protocol |
| WB protocol for HIF-1 alpha antibody 66730-1-Ig | Download protocol |
| human | WB Theranostics ITGB2-mediated metabolic switch in CAFs promotes OSCC proliferation by oxidation of NADH in mitochondrial oxidative phosphorylation system. Authors - Xiaoxin Zhang View Article |
| P (Verified Customer) (01-29-2026) | Good Applications: Western Blot Primary Antibody Dilution: 1000 Cell Tissue Type: H441 HIF-1 alpha Antibody Western Blot validation (1000 dilution) in H441 (Cat no:66730-1-Ig) |
| S (Verified Customer) (05-16-2024) | Excellent Applications: Western Blot Primary Antibody Dilution: 1000 Cell Tissue Type: AC16 HIF-1 alpha Antibody Western Blot validation (1000 dilution) in AC16 (Cat no:66730-1-Ig) |
| balawant (Verified Customer) (12-20-2022) | I have used this antibody for western blot and it is working great Applications: Western Blot Primary Antibody Dilution: 1:1000 Cell Tissue Type: renal Cancer cell line |
| Diane (Verified Customer) (02-02-2021) | incubated over night 4 degrees C. 1:2500 HRP conjugated secondary. Opti-4CN substrate kit for detection. Also tried ICC on cytospins so ran out of trial size before fully optimized but has potential to provide the information that I am needing. Applications: Western Blot Primary Antibody Dilution: 1:1250 Cell Tissue Type: Cell lysates glioblastoma primary cells HIF-1 alpha Antibody Western Blot validation (1:1250 dilution) in Cell lysates glioblastoma primary cells (Cat no:66730-1-Ig) |
| Citations | 108 |
| Dilutions | WB : 1:2000-1:10000 IHC : 1:50-1:500 |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Clone Number | 1H3C12 |
| Conjugation | Unconjugated |
HIF1a, the major regulator of the cellular responses to hypoxia, consists of an oxygen-sensitive subunit, HIF1 alpha (HIF1A), and an oxygen-insensitive subunit, HIF1 beta (arylhydrocarbon receptor nuclear transporter [ARNT]). Under normal oxygen conditions, HIF1a is continuously produced and destroyed, in a process involving hydroxylation, interaction with von Hippel-Lindau (VHL) protein, polyubiquitylation and subsequent proteasomal degradation. Under hypoxic conditions, hydroxylation is impaired and HIF1a is stabilized. HIF1a localizes in cytoplasm in normoxia, but it can translocate into nuclear in response to hypoxia. The calculated molecular weight of HIF1a is 93 kDa, but the modified protein HIF1a is about 110-120kDa (PMID: 11698256, .PMID: 7539918). . Protocols Product Specific Protocols IHC protocol for HIF-1 alpha antibody 66730-1-Ig Download protocol WB protocol for HIF-1 alpha antibody 66730-1-Ig Download protocol Standard Protocols Click here to view our Standard Protocols
Publications
- Transgelin Promotes Glioblastoma Stem Cell Hypoxic Responses and Maintenance Through p53 Acetylation
Journal: Adv Sci (Weinh) | Authors: Huan Li | Species: human | Application: WB,IP,IHC,IF - 124I-labelled BMSC-Derived Extracellular Vesicles Deliver CRISPR/Cas9 Ribonucleoproteins With a GFP-Reporter System to Inhibit Osteosarcoma Proliferation and Metastasis
Journal: J Extracell Vesicles | Authors: Yujie Pan | Species: human | Application: WB - Ultrasound-visible engineered bacteria for tumor chemo-immunotherapy
Journal: Cell Rep Med | Authors: Yaozhang Yang | Application: IF - TSH-TSHR axis promotes tumor immune evasion.
Journal: J Immunother Cancer | Authors: Zhenghao Wu | Species: human | Application: WB - ITGB2-mediated metabolic switch in CAFs promotes OSCC proliferation by oxidation of NADH in mitochondrial oxidative phosphorylation system.
Journal: Theranostics | Authors: Xiaoxin Zhang | Species: human | Application: WB - mTOR/HIF-1α pathway-mediated glucose reprogramming and macrophage polarization by Sini decoction plus ginseng soup in ALF
Journal: Phytomedicine | Authors: Junli Zhang | Application: WB
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