CD90.1 Recombinant monoclonal antibody 84832-1-RR proteintech
$149.00
In stock
SKU
84832-1-RR
| Catalog Number | 84832-1-RR |
| Synonyms | CD90, Thy1, Thy 1, 241542D7 |
| Host | Rabbit |
| Reactivity | mouse |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, IHC, IF-P, ELISA |
| Host / Isotype | Rabbit / IgG |
| Tested Applications — Positive WB detected in | FVB mouse brain tissue, FVB mouse spleen tissue |
| Tested Applications — Positive IHC detected in | mouse spleen tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Tested Applications — Positive IF-P detected in | mouse spleen tissue |
| Recommended Dilutions — Western Blot (WB) | WB : 1:5000-1:50000 |
| Recommended Dilutions — Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
| Recommended Dilutions — Immunofluorescence (IF)-P | IF-P : 1:250-1:1000 |
| Positive WB detected in | FVB mouse brain tissue, FVB mouse spleen tissue |
| Positive IHC detected in | mouse spleen tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse spleen tissue |
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
| Immunofluorescence (IF)-P | IF-P : 1:250-1:1000 |
| Tested Reactivity | mouse |
| Class | Recombinant |
| Type | Antibody |
| Immunogen | Recombinant protein Predict reactive species |
| Full Name | thymus cell antigen 1, theta |
| Observed Molecular Weight | 25 kDa |
| Gene Symbol | CD90 |
| Gene ID (NCBI) | 21838 |
| RRID | AB_3672141 |
| Conjugate | Unconjugated |
| Purification Method | Protein A purfication |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| IF protocol for CD90.1 antibody 84832-1-RR | Download protocol |
| IHC protocol for CD90.1 antibody 84832-1-RR | Download protocol |
| WB protocol for CD90.1 antibody 84832-1-RR | Download protocol |
| Citations | - |
| Dilutions | WB : 1:5000-1:50000 IHC : 1:1000-1:4000 IF-P : 1:250-1:1000 |
| Isotype | IgG |
| Clonality | Recombinant |
| Clone Number | 241542D7 |
| Conjugation | Unconjugated |
CD90 (Thy-1) is a 25 kDa, GPI-linked membrane glycoprotein that belongs to immunoglobulin superfamily (PMID: 6177036; 6153212). Originally described as a brain thymus cross-reactive antigen, it is found in large quantities on mouse and rat thymocytes and central nervous system cells (PMID: 83175). CD90 has been postulated to be involved in cellular recognition, adherence, and T cell activation (PMID: 7683034). Mouse CD90 (Thy-1) is defined as a differentiation alloantigen, represented as two serologically distinguishable allelic forms, designated CD90.1 (Thy-1.1) and CD90.2 (Thy-1.2) (PMID: 83175). CD90.2 is expressed by thymocytes, T cells, neurons and hematopoietic stem cells in most strains of mice (C3H, BALB/c, C57BL/6, DBA, and others), whereas CD90.1 is expressed in some mouse strains like AKR and FVB (PMID: 5919593). Protocols Product Specific Protocols IF protocol for CD90.1 antibody 84832-1-RR Download protocol IHC protocol for CD90.1 antibody 84832-1-RR Download protocol WB protocol for CD90.1 antibody 84832-1-RR Download protocol Standard Protocols Click here to view our Standard Protocols
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