CDC5L Polyclonal antibody 12974-1-AP proteintech
$149.00
In stock
SKU
12974-1-AP
| Catalog Number | 12974-1-AP |
| Synonyms | PCDC5RP, KIAA0432, Cell division cycle 5-like protein, CEF1, Cdc5-like protein |
| Host | Rabbit |
| Reactivity | human, mouse |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, IHC, IF/ICC, IP, CoIP, RIP, ELISA |
| Applications | WB, IHC, IF/ICC, IP, ELISA |
| Host / Isotype | Rabbit / IgG |
| Tested Applications — Positive WB detected in | human brain tissue, HEK-293 cells, HepG2 cells, mouse brain tissue |
| Tested Applications — Positive IP detected in | mouse brain tissue |
| Tested Applications — Positive IHC detected in | mouse testis tissue, mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Tested Applications — Positive IF/ICC detected in | HepG2 cells |
| Recommended Dilutions — Western Blot (WB) | WB : 1:500-1:2000 |
| Recommended Dilutions — Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Recommended Dilutions — Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Recommended Dilutions — Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| Positive WB detected in | human brain tissue, HEK-293 cells, HepG2 cells, mouse brain tissue |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | mouse testis tissue, mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CatNo: Ag3578 Product name: Recombinant human CDC5L protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 451-803 aa of BC001568 Sequence: NPEDGMADYSDPSYVKQMERESREHLRLGLLGLPAPKNDFEIVLPENAEKELEEREIDDTYIEDAADVDARKQAIRDAERVKEMKRMHKAVQKDLPRPSEVNETILRPLNVEPPLTDLQKSEELIKKEMITMLHYDLLHHPYEPSGNKKGKTVGFGTNNSEHITYLEHNPYEKFSKEELKKAQDVLVQEMEVVKQGMSHGELSSEAYNQVWEECYSQVLYLPGQSRYTRANLASKKDRIESLEKRLEINRGHMTTEAKRAAKMEKKMKILLGGYQSRAMGLMKQLNDLWDQIEQAHLELRTFEELKKHEDSAIPRRLECLKEDVQRQQEREKELQHRYADLLLEKETLKSKF Predict reactive species |
| Full Name | CDC5 cell division cycle 5-like (S. pombe) |
| Calculated Molecular Weight | 802 aa, 92 kDa |
| Observed Molecular Weight | 100 kDa |
| GenBank Accession Number | BC001568 |
| Gene Symbol | CDC5L |
| Gene ID (NCBI) | 988 |
| RRID | AB_2078510 |
| Conjugate | Unconjugated |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q99459 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| IF protocol for CDC5L antibody 12974-1-AP | Download protocol |
| IHC protocol for CDC5L antibody 12974-1-AP | Download protocol |
| IP protocol for CDC5L antibody 12974-1-AP | Download protocol |
| WB protocol for CDC5L antibody 12974-1-AP | Download protocol |
| human | WB Apoptosis LncRNA AC098613.1 promotes acute myeloid leukemia cell differentiation through CDC5L/ADAP1/NRD1 axis. Authors - Qingling Jiang View Article |
| mouse | WB Acta Biochim Biophys Sin (Shanghai) CDC5L facilitates cardiomyocyte proliferation and ameliorates myocardial ischemia-reperfusion injury via modulation of the FGF10-YAP axis. Authors - Tianwen Wei View Article |
| Wael (Verified Customer) (01-19-2023) | CDC5L detected in both nuclear and cytoplasmic fractions of Hek293 lysates. specific band between 100 and 130kd. Applications: Western Blot Primary Antibody Dilution: 1:2000 Cell Tissue Type: Hek293 CDC5L Antibody Western Blot validation (1:2000 dilution) in Hek293 (Cat no:12974-1-AP) |
| Citations | 7 |
| Dilutions | WB : 1:500-1:2000 IP : 0.5-4.0 ug for IP and 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate for WB IHC : 1:50-1:500 IF/ICC : 1:200-1:800 |
| Isotype | IgG |
| Clonality | Polyclonal |
| Clone Number | - |
| Conjugation | Unconjugated |
Publications
- CDC5L drives FAH expression to promote metabolic reprogramming in melanoma.
Journal: Oncotarget | Authors: Zhichao Gu | Species: human | Application: WB - O-GlcNAcylation of Thr12 / Ser56 in short form O-GlcNAc transferase (sOGT) regulates its substrate selectivity.
Journal: J Biol Chem | Authors: Li Liu | Application: WB - Circ-PGAM1 promotes malignant progression of epithelial ovarian cancer through regulation of the miR-542-3p/CDC5L/PEAK1 pathway.
Journal: Cancer Med | Authors: Chunmei Zhang | Species: human | Application: WB - CDC5L binds to ELAVL1 to inhibit pyroptosis in hepatocellular carcinoma through the Caspase 3/GSDME signaling pathway.
Journal: Int J Mol Med | Authors: Shuai Liang KD Validated | Species: human | Application: WB,RIP,CoIP - LncRNA AC098613.1 promotes acute myeloid leukemia cell differentiation through CDC5L/ADAP1/NRD1 axis.
Journal: Apoptosis | Authors: Qingling Jiang | Species: human | Application: WB - CDC5L facilitates cardiomyocyte proliferation and ameliorates myocardial ischemia-reperfusion injury via modulation of the FGF10-YAP axis.
Journal: Acta Biochim Biophys Sin (Shanghai) | Authors: Tianwen Wei | Species: mouse | Application: WB
Reviews
Write Your Own Review
Related Products
Check items to add to the cart or
