CEA/CD66e Polyclonal antibody 31519-1-AP proteintech
$149.00
In stock
SKU
31519-1-AP
| Catalog Number | 31519-1-AP |
| Synonyms | CEA, Carcinoembryonic antigen, Carcinoembryonic antigen-related cell adhesion molecule 5, CD66e, CEACAM5 |
| Host | Rabbit |
| Reactivity | human |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, IHC, ELISA |
| Host / Isotype | Rabbit / IgG |
| Tested Applications — Positive WB detected in | HT-29 cells |
| Tested Applications — Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Recommended Dilutions — Western Blot (WB) | WB : 1:5000-1:50000 |
| Recommended Dilutions — Immunohistochemistry (IHC) | IHC : 1:2000-1:8000 |
| Positive WB detected in | HT-29 cells |
| Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:2000-1:8000 |
| Tested Reactivity | human |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant protein Predict reactive species |
| Full Name | carcinoembryonic antigen-related cell adhesion molecule 5 |
| Calculated Molecular Weight | 77 kDa |
| Observed Molecular Weight | 200 kDa |
| GenBank Accession Number | NM_004363 |
| Gene Symbol | CEA |
| Gene ID (NCBI) | 1048 |
| RRID | AB_3742444 |
| Conjugate | Unconjugated |
| Purification Method | Antigen affinity Purification |
| UNIPROT ID | P06731-1 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| IHC protocol for CEA/CD66e antibody 31519-1-AP | Download protocol |
| WB protocol for CEA/CD66e antibody 31519-1-AP | Download protocol |
| Citations | - |
| Dilutions | WB : 1:5000-1:50000 IHC : 1:2000-1:8000 |
| Isotype | IgG |
| Clonality | Polyclonal |
| Clone Number | - |
| Conjugation | Unconjugated |
Carcinoembryonic antigen (CEA), also known as CEACAM5 or CD66e, is a cell surface glycoprotein belonging to the immunoglobulin superfamily, mainly serving as a cell adhesion molecule mediating intercellular contact by both homophilic and heterophilic binding (PMID: 21731662). CEA inhibits anoikis and plays a role in tumorigenesis and metastasis. CEA has been found to be overexpressed in a wide variety of human cancers, including colon, breast, and lung (PMID: 17167768). CEA is a tumor marker and is routinely exploited for diagnosis. Protocols Product Specific Protocols IHC protocol for CEA/CD66e antibody 31519-1-AP Download protocol WB protocol for CEA/CD66e antibody 31519-1-AP Download protocol Standard Protocols Click here to view our Standard Protocols
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