Cingulin Polyclonal antibody 21369-1-AP proteintech
$149.00
In stock
SKU
21369-1-AP
| Catalog Number | 21369-1-AP |
| Synonyms | CGN, KIAA1319 |
| Host | Rabbit |
| Reactivity | human, mouse, rat and More (1) |
| Reactivity | human, mouse, rat, canine |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, IHC, IF, IP, ELISA |
| Applications | WB, IHC, IP, ELISA |
| Host / Isotype | Rabbit / IgG |
| Tested Applications — Positive WB detected in | HEK-293T cells, A431 cells, MCF-7 cells, mouse kidney tissue, mouse small intestine tissue, Jurkat cells, mouse colon tissue, HeLa cells |
| Tested Applications — Positive IP detected in | mouse kidney tissue |
| Tested Applications — Positive IHC detected in | human ovary cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Recommended Dilutions — Western Blot (WB) | WB : 1:2000-1:10000 |
| Recommended Dilutions — Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Recommended Dilutions — Immunohistochemistry (IHC) | IHC : 1:400-1:1600 |
| Positive WB detected in | HEK-293T cells, A431 cells, MCF-7 cells, mouse kidney tissue, mouse small intestine tissue, Jurkat cells, mouse colon tissue, HeLa cells |
| Positive IP detected in | mouse kidney tissue |
| Positive IHC detected in | human ovary cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:400-1:1600 |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, canine |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CatNo: Ag15843 Product name: Recombinant human CGN protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-350 aa of BC146657 Sequence: MAEPRGPVDHGVQIRFITEPVSGAEMGTLRRGGRRPAKDARASTYGVAVRVQGIAGQPFVVLNSGEKGGDSFGVQIKGANDQGASGALSSDLELPENPYSQVKGFPAPSQSSTSDEEPGAYWNGKLLRSHSQASLAGPGPVDPSNRSNSMLELAPKVASPGSTIDTAPLSSVDSLINKFDSQLGGQARGRTGRRTRMLPPEQRKRSKSLDSRLPRDTFEERERQSTNHWTSSTKYDNHVGTSKQPAQSQNLSPLSGFSRSRQTQDWVLQSFEEPRRSAQDPTMLQFKSTPDLLRDQQEAAPPGSVDHMKATIYGILREGSSESETSVRRKVSLVLEKMQPLVMVSSGSTK Predict reactive species |
| Full Name | cingulin |
| Calculated Molecular Weight | 1197 aa, 136 kDa |
| Observed Molecular Weight | 136-150 kDa |
| GenBank Accession Number | BC146657 |
| Gene Symbol | Cingulin |
| Gene ID (NCBI) | 57530 |
| RRID | AB_10733647 |
| Conjugate | Unconjugated |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9P2M7 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| IHC protocol for Cingulin antibody 21369-1-AP | Download protocol |
| IP protocol for Cingulin antibody 21369-1-AP | Download protocol |
| WB protocol for Cingulin antibody 21369-1-AP | Download protocol |
| human | WB,IHC Cancers (Basel) miR-125b Promotes Colorectal Cancer Migration and Invasion by Dual-Targeting CFTR and CGN. Authors - Xiaohui Zhang View Article |
| canine | WB,IF Nat Commun Cingulin and actin mediate midbody-dependent apical lumen formation during polarization of epithelial cells. Authors - Anthony J Mangan KO Validated View Article |
| mouse | WB,IP Cell Signal Motif affinity and mass spectrometry proteomic approach for the discovery of cellular AMPK targets: Identification of mitochondrial fission factor as a new AMPK substrate. Authors - Serge Ducommun View Article |
| rat | WB Sci Rep Treatment with Ligilactobacillus murinus lowers blood pressure and intestinal permeability in spontaneously hypertensive rats Authors - Masashi Mukohda View Article |
| Citations | 13 |
| Dilutions | WB : 1:2000-1:10000 IP : 0.5-4.0 ug for IP and 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate for WB IHC : 1:400-1:1600 |
| Isotype | IgG |
| Clonality | Polyclonal |
| Clone Number | - |
| Conjugation | Unconjugated |
Cingulin is a component of the cytoplasmic plaque of tight junction, and is localized on the cytoplasmic face of tight junctions of polarized epithelia and some endothelia. Cingulin is a homodimer, each subunit containing a globular "head" domain, a coiled-coil "rod" domain and a small globular "tail" region. Cingulin can interact interact with ZO-1, ZO-2, ZO-3, actin, and myosin. It probably plays a role in the formation and regulation of the tight junction paracellular permeability barrier. Protocols Product Specific Protocols IHC protocol for Cingulin antibody 21369-1-AP Download protocol IP protocol for Cingulin antibody 21369-1-AP Download protocol WB protocol for Cingulin antibody 21369-1-AP Download protocol Standard Protocols Click here to view our Standard Protocols
Publications
- The tight junction protein TJP1 regulates the feeding-modulated hepatic circadian clock.
Journal: Nat Commun | Authors: Yi Liu | Species: human | Application: WB - Cingulin and actin mediate midbody-dependent apical lumen formation during polarization of epithelial cells.
Journal: Nat Commun | Authors: Anthony J Mangan KO Validated | Species: canine | Application: WB,IF - Epithelial NELF guards intestinal barrier function to ameliorate colitis by maintaining junctional integrity.
Journal: Mucosal Immunol | Authors: Jiayao Ou | Species: mouse | Application: WB,IF - miR-125b Promotes Colorectal Cancer Migration and Invasion by Dual-Targeting CFTR and CGN.
Journal: Cancers (Basel) | Authors: Xiaohui Zhang | Species: human | Application: WB,IHC - Treatment with Ligilactobacillus murinus lowers blood pressure and intestinal permeability in spontaneously hypertensive rats
Journal: Sci Rep | Authors: Masashi Mukohda | Species: rat | Application: WB - Motif affinity and mass spectrometry proteomic approach for the discovery of cellular AMPK targets: Identification of mitochondrial fission factor as a new AMPK substrate.
Journal: Cell Signal | Authors: Serge Ducommun | Species: mouse | Application: WB,IP - Epithelial NELF guards intestinal barrier function to ameliorate colitis by maintaining junctional integrity.
Journal: Mucosal Immunol | Authors: Jiayao Ou | Species: mouse | Application: WB,IF - Treatment with Ligilactobacillus murinus lowers blood pressure and intestinal permeability in spontaneously hypertensive rats
Journal: Sci Rep | Authors: Masashi Mukohda | Species: rat | Application: WB - miR-125b Promotes Colorectal Cancer Migration and Invasion by Dual-Targeting CFTR and CGN.
Journal: Cancers (Basel) | Authors: Xiaohui Zhang | Species: human | Application: WB,IHC - Geniposide Protects Against Myocardial Infarction Injury via the Restoration in Gut Microbiota and Gut-Brain Axis
Journal: J Cell Mol Med | Authors: Jie Chen | Species: mouse | Application: IF - Motif affinity and mass spectrometry proteomic approach for the discovery of cellular AMPK targets: Identification of mitochondrial fission factor as a new AMPK substrate.
Journal: Cell Signal | Authors: Serge Ducommun | Species: mouse | Application: WB,IP - Tanshinone IIA Exerts Cardioprotective Effects Through Improving Gut-Brain Axis Post-Myocardial Infarction
Journal: Cardiovasc Toxicol | Authors: Tong Zhu | Species: mouse | Application: IF - Raptor directs Sertoli cell cytoskeletal organization and polarity in the mouse testis.
Journal: Biol Reprod | Authors: Zhi Xiong | Species: mouse | Application: IF
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