CHFR Polyclonal antibody 12169-1-AP proteintech
$149.00
In stock
SKU
12169-1-AP
| Catalog Number | 12169-1-AP |
| Synonyms | CHFR, RING finger protein 196, RNF116, RNF196 |
| Host | Rabbit |
| Reactivity | human, mouse |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, IHC, ELISA |
| Host / Isotype | Rabbit / IgG |
| Tested Applications — Positive WB detected in | HepG2 cells, multi-cells/tissue |
| Tested Applications — Positive IHC detected in | human endometrial cancer tissue, human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Recommended Dilutions — Western Blot (WB) | WB : 1:500-1:2000 |
| Recommended Dilutions — Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Positive WB detected in | HepG2 cells, multi-cells/tissue |
| Positive IHC detected in | human endometrial cancer tissue, human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CatNo: Ag2811 Product name: Recombinant human CHFR protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 344-652 aa of BC012072 Sequence: NNLVEAYLIQHPDKSRSEEDVQSMDARNKITQDMLQPKVRRSFSDEEGSSEDLLELSDVDSESSDISQPYVVCRQCPEYRRQAAQPPHCPAPEGEPGAPQALGDAPSTSVSLTTAVQDYVCPLQGSHALCTCCFQPMPDRRVEREQDPRVAPQQCAVCLQPFCHLYWGCTRTGCYGCLAPFCELNLGDKCLDGVLNNNSYESDILKNYLATRGLTWKNMLTESLVALQRGVFLLSDYRVTGDTVLCYCCGLRSFRELTYQYRQNIPASELPVAVTSRPDCYWGRNCRTQVKAHHAMKFNHICEQTRFKN Predict reactive species |
| Full Name | checkpoint with forkhead and ring finger domains |
| Calculated Molecular Weight | 664 aa, 73 kDa |
| Observed Molecular Weight | 70-85 kDa |
| GenBank Accession Number | BC012072 |
| Gene Symbol | CHFR |
| Gene ID (NCBI) | 55743 |
| RRID | AB_2080821 |
| Conjugate | Unconjugated |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q96EP1 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| IHC protocol for CHFR antibody 12169-1-AP | Download protocol |
| WB protocol for CHFR antibody 12169-1-AP | Download protocol |
| human | WB Mol Med Rep CHFR‑mediated epithelial‑to‑mesenchymal transition promotes metastasis in human breast cancer cells. Authors - Guobin Jiang KD Validated View Article |
| human,mouse | WB Nat Commun Ubiquitin ligase CHFR mediated degradation of VE-cadherin through ubiquitylation disrupts endothelial adherens junctions Authors - Chinnaswamy Tiruppathi KD Validated View Article |
| Mounika (Verified Customer) (01-08-2026) | these antibodies are excellent, bands and its brightness are accurate! Applications: Western Blot, Immunohistochemistry, Immunofluorescence, Immunoprecipitation Primary Antibody Dilution: 1:500 |
| Iram (Verified Customer) (02-11-2022) | This antibody works for me great Applications: Western Blot, Immunofluorescence Primary Antibody Dilution: 1:1000 for western and 1:500 for IF Cell Tissue Type: Colon cancer cells |
| Citations | 8 |
| Dilutions | WB : 1:500-1:2000 IHC : 1:20-1:200 |
| Isotype | IgG |
| Clonality | Polyclonal |
| Clone Number | - |
| Conjugation | Unconjugated |
The Checkpoint with forkhead and RING finger domains protein(CHFR) is a checkpoint protein that reportedly initiates a cell cycle delay in response to microtubule stress during prophase in mitosis and it has been hypothesized to be a tumor suppressor with a potential role as a biomarker for chemotherapeutic response to Taxol(PMID:17596595). It belongs to the CHFR family. Endogenous Chfr is ubiquitinated and phosphorylated in an MPF-dependent manner in mitotic Xenopus egg extracts(PMID:18504434). It has 5 isoforms produced by alternative splicing. Protocols Product Specific Protocols IHC protocol for CHFR antibody 12169-1-AP Download protocol WB protocol for CHFR antibody 12169-1-AP Download protocol Standard Protocols Click here to view our Standard Protocols
Publications
- Association of CHFR promoter methylation with disease recurrence in locally advanced colon cancer.
Journal: Clin Cancer Res | Authors: Tanaka Motofumi M | Species: human | Application: IHC - Silencing of CHFR Sensitizes Gastric Carcinoma to PARP Inhibitor Treatment.
Journal: Transl Oncol | Authors: Yuan Li | Species: human | Application: WB - CHFR protein regulates mitotic checkpoint by targeting PARP-1 protein for ubiquitination and degradation.
Journal: J Biol Chem | Authors: Kashima Lisa L | Species: human | Application: IHC - Identification of a Novel Epigenetic Signature CHFR as a Potential Prognostic Gene Involved in Metastatic Clear Cell Renal Cell Carcinoma.
Journal: Front Genet | Authors: Xiangling Chen KD Validated | Species: human | Application: WB - CHFR‑mediated epithelial‑to‑mesenchymal transition promotes metastasis in human breast cancer cells.
Journal: Mol Med Rep | Authors: Guobin Jiang KD Validated | Species: human | Application: WB - Ubiquitin ligase CHFR mediated degradation of VE-cadherin through ubiquitylation disrupts endothelial adherens junctions
Journal: Nat Commun | Authors: Chinnaswamy Tiruppathi KD Validated | Species: human,mouse | Application: WB
Reviews
Write Your Own Review
Related Products
Check items to add to the cart or
