Chordin Polyclonal antibody 27293-1-AP proteintech
$149.00
In stock
SKU
27293-1-AP
| Catalog Number | 27293-1-AP |
| Synonyms | chordin, CHRD |
| Host | Rabbit |
| Reactivity | human, mouse, rat |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, IHC, ELISA |
| Host / Isotype | Rabbit / IgG |
| Tested Applications — Positive WB detected in | 3T3-L1 cells |
| Tested Applications — Positive IHC detected in | human liver cancer tissue, rat brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Recommended Dilutions — Western Blot (WB) | WB : 1:200-1:1000 |
| Recommended Dilutions — Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Positive WB detected in | 3T3-L1 cells |
| Positive IHC detected in | human liver cancer tissue, rat brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CatNo: Ag26001 Product name: Recombinant human CHRD protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 27-350 aa of BC112345 Sequence: AGPEPPVLPIRSEKEPLPVRGAAGCTFGGKVYALDETWHPDLGEPFGVMRCVLCACEAPQWGRRTRGPGRVSCKNIKPECPTPACGQPRQLPGHCCQTCPQERSSSERQPSGLSFEYPRDPEHRSYSDRGEPGAEERARGDGHTDFVALLTGPRSQAVARARVSLLRSSLRFSISYRRLDRPTRIRFSDSNGSVLFEHPAAPTQDGLVCGVWRAVPRLSLRLLRAEQLHVALVTLTHPSGEVWGPLIRHRALAAETFSAILTLEGPPQQGVGGITLLTLSDTEDSLHFLLLFRGLLEPRSGGLTQVPLRLQILHQGQLLRELQA Predict reactive species |
| Full Name | chordin |
| Calculated Molecular Weight | 955 aa, 102 kDa |
| Observed Molecular Weight | 102 kDa |
| GenBank Accession Number | BC112345 |
| Gene Symbol | Chordin |
| Gene ID (NCBI) | 8646 |
| RRID | AB_2880832 |
| Conjugate | Unconjugated |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9H2X0 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| IHC protocol for Chordin antibody 27293-1-AP | Download protocol |
| WB protocol for Chordin antibody 27293-1-AP | Download protocol |
| human | IHC Oncogene Suppression of lysosome metabolism-meditated GARP/TGF-β1 complexes specifically depletes regulatory T cells to inhibit breast cancer metastasis Authors - Jing Ma View Article |
| Citations | 1 |
| Dilutions | WB : 1:200-1:1000 IHC : 1:50-1:500 |
| Isotype | IgG |
| Clonality | Polyclonal |
| Clone Number | - |
| Conjugation | Unconjugated |
CHRD is a secreted protein that dorsalizes early vertebrate embryonic tissues by binding to ventralizing TGF-beta-like bone morphogenetic proteins and sequestering them in latent complexes. CHRD may also have roles in organogenesis and during adulthood. It has been suggested that CHRD could be a candidate gene for Cornelia de Lange syndrome. Reduced expression of CHRD results in enhanced bone regeneration. Protocols Product Specific Protocols IHC protocol for Chordin antibody 27293-1-AP Download protocol WB protocol for Chordin antibody 27293-1-AP Download protocol Standard Protocols Click here to view our Standard Protocols
Publications
- Suppression of lysosome metabolism-meditated GARP/TGF-β1 complexes specifically depletes regulatory T cells to inhibit breast cancer metastasis
Journal: Oncogene | Authors: Jing Ma | Species: human | Application: IHC
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