CHRM3 Polyclonal antibody 21978-1-AP proteintech
$149.00
In stock
SKU
21978-1-AP
| Catalog Number | 21978-1-AP |
| Synonyms | HM3 |
| Host | Rabbit |
| Reactivity | human, mouse, rat |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, IHC, ELISA |
| Host / Isotype | Rabbit / IgG |
| Tested Applications — Positive WB detected in | SH-SY5Y cells, mouse brain tissue, rat brain tissue, SK-N-SH cells |
| Tested Applications — Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Recommended Dilutions — Western Blot (WB) | WB : 1:500-1:2000 |
| Recommended Dilutions — Immunohistochemistry (IHC) | IHC : 1:200-1:1000 |
| Positive WB detected in | SH-SY5Y cells, mouse brain tissue, rat brain tissue, SK-N-SH cells |
| Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:200-1:1000 |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | mouse |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CatNo: Ag17791 Product name: Recombinant human CHRM3 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 261-477 aa of BC121026 Sequence: RTKELAGLQASGTEAETENFVHPTGSSRSCSSYELQQQSMKRSNRRKYGRCHFWFTTKSWKPSSEQMDQDHSSSDSWNNNDAAASLENSASSDEEDIGSETRAIYSIVLKLPGHSTILNSTKLPSSDNLQVPEEELGMVDLERKADKLQAQKSVDDGGSFPKSFSKLPIQLESAVDTAKTSDVNSSVGKSTATLPLSFKEATLAKRFALKTRSQITK Predict reactive species |
| Full Name | cholinergic receptor, muscarinic 3 |
| Calculated Molecular Weight | 590 aa, 66 kDa |
| Observed Molecular Weight | 60-66 kDa |
| GenBank Accession Number | BC121026 |
| Gene Symbol | CHRM3 |
| Gene ID (NCBI) | 1131 |
| RRID | AB_3085681 |
| Conjugate | Unconjugated |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P20309 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| IHC protocol for CHRM3 antibody 21978-1-AP | Download protocol |
| WB protocol for CHRM3 antibody 21978-1-AP | Download protocol |
| mouse | WB Glia The Histone Methyltransferases EHMT1 and EHMT2 Repress the Expression of Genes Related to Excitability and Cell Death in Oligodendrocyte Progenitors Authors - Mathilde Pruvost View Article |
| Salma (Verified Customer) (07-27-2026) | Good antibody, worked well for chemiluminescent Western Blotting. Applications: Western Blot |
| Hamidreza (Verified Customer) (10-08-2025) | The western blot is attached to this email Applications: Western Blot CHRM3 Antibody Western Blot validation ( dilution) in (Cat no:21978-1-AP) |
| Yi Han (Verified Customer) (10-08-2025) | Samples used are synaptosome fractions from human neurons (all 6 lanes). Tuj1 as loading control. Clean band corresponding to the right MW. Applications: Western Blot Primary Antibody Dilution: 1:1000 Cell Tissue Type: human neurons CHRM3 Antibody Western Blot validation (1:1000 dilution) in human neurons (Cat no:21978-1-AP) |
| Citations | 1 |
| Dilutions | WB : 1:500-1:2000 IHC : 1:200-1:1000 |
| Isotype | IgG |
| Clonality | Polyclonal |
| Clone Number | - |
| Conjugation | Unconjugated |
CHRM3 (M3 muscarinic acetylcholine receptor) is a major player in many kinds of cancer (PMID: 26175851). The expression of muscarinic receptors, mainly the CHRM3, is amplified in several types of tumors, including colon cancer, prostate cancer, endometrial carcinoma, and gastric cancer. Colon cancer overexpresses CHRM3, and post-M3R signaling promotes cell growth via the activation of the epidermal growth factor receptors/ERK and protein kinase C/p38 mitogen-activated protein kinase (MAPK) signaling pathways (PMID: 37744266). Protocols Product Specific Protocols IHC protocol for CHRM3 antibody 21978-1-AP Download protocol WB protocol for CHRM3 antibody 21978-1-AP Download protocol Standard Protocols Click here to view our Standard Protocols
Publications
- The Histone Methyltransferases EHMT1 and EHMT2 Repress the Expression of Genes Related to Excitability and Cell Death in Oligodendrocyte Progenitors
Journal: Glia | Authors: Mathilde Pruvost | Species: mouse | Application: WB
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