CLIP-115/CLIP2 Polyclonal antibody 25088-1-AP proteintech
$149.00
In stock
SKU
25088-1-AP
| Catalog Number | 25088-1-AP |
| Synonyms | CLIP 115, CLIP2, CAP-Gly domain-containing linker protein 2, CLIP-115, KIAA0291 |
| Host | Rabbit |
| Reactivity | human, mouse, rat |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, IHC, IF, IP, ELISA |
| Applications | WB, IHC, IP, ELISA |
| Host / Isotype | Rabbit / IgG |
| Tested Applications — Positive WB detected in | RAW 264.7 cells, A549 cells, rat brain tissue, mouse brain tissue, mouse cerebellum tissue, rat cerebellum tissue |
| Tested Applications — Positive IP detected in | mouse brain tissue |
| Tested Applications — Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Recommended Dilutions — Western Blot (WB) | WB : 1:5000-1:50000 |
| Recommended Dilutions — Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Recommended Dilutions — Immunohistochemistry (IHC) | IHC : 1:400-1:1600 |
| Positive WB detected in | RAW 264.7 cells, A549 cells, rat brain tissue, mouse brain tissue, mouse cerebellum tissue, rat cerebellum tissue |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:400-1:1600 |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CatNo: Ag18692 Product name: Recombinant human CLIP2 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 671-1011 aa of BC150332 Sequence: QLEVQASQHRLELQEAQDQRRDAELRVHELEKLDVEYRGQAQAIEFLKEQISLAEKKMLDYERLQRAEAQGKQEVESLREKLLVAENRLQAVEALCSSQHTHMIESNDISEETIRTKETVEGLQDKLNKRDKEVTALTSQTEMLRAQVSALESKCKSGEKKVDALLKEKRRLEAELETVSRKTHDASGQLVLISQELLRKERSLNELRVLLLEANRHSPGPERDLSREVHKAEWRIKEQKLKDDIRGLREKLTGLDKEKSLSDQRRYSLIDPSSAPELLRLQHQLMSTEDALRDALDQAQQVEKLMEAMRSCPDKAQTIGNSGSANGIHQQDKAQKQEDKH Predict reactive species |
| Full Name | CAP-GLY domain containing linker protein 2 |
| Calculated Molecular Weight | 1011 aa, 112 kDa |
| Observed Molecular Weight | 110-120 kDa |
| GenBank Accession Number | BC150332 |
| Gene Symbol | CLIP2 |
| Gene ID (NCBI) | 7461 |
| RRID | AB_2879890 |
| Conjugate | Unconjugated |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9UDT6 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| IHC protocol for CLIP-115/CLIP2 antibody 25088-1-AP | Download protocol |
| IP protocol for CLIP-115/CLIP2 antibody 25088-1-AP | Download protocol |
| WB protocol for CLIP-115/CLIP2 antibody 25088-1-AP | Download protocol |
| mouse | IF Curr Biol Stretch triggers microtubule stabilization and MARCKS-dependent membrane incorporation in the shaft of embryonic axons Authors - Sara C Sousa View Article |
| mouse,human | WB,IF Cell Commun Signal CLIP2: a novel functional player in AQP5 trafficking dynamics and implications for Sjögren's disease. Authors - Claudia D'Agostino KO Validated View Article |
| Citations | 2 |
| Dilutions | WB : 1:5000-1:50000 IP : 0.5-4.0 ug for IP and 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate for WB IHC : 1:400-1:1600 |
| Isotype | IgG |
| Clonality | Polyclonal |
| Clone Number | - |
| Conjugation | Unconjugated |
CILP-2/CLIP-115 is a cytoplasmic linker protein (CLIP) that links membranous organelles to microtubules. CLIP-115 is enriched in the axonal growth cone and it enables neuronal polarization by controlling the stabilization of microtubules. Mutation of CLIP-115 has been associated with Williams-Beuren syndrome. Protocols Product Specific Protocols IHC protocol for CLIP-115/CLIP2 antibody 25088-1-AP Download protocol IP protocol for CLIP-115/CLIP2 antibody 25088-1-AP Download protocol WB protocol for CLIP-115/CLIP2 antibody 25088-1-AP Download protocol Standard Protocols Click here to view our Standard Protocols
Publications
- Stretch triggers microtubule stabilization and MARCKS-dependent membrane incorporation in the shaft of embryonic axons
Journal: Curr Biol | Authors: Sara C Sousa | Species: mouse | Application: IF - CLIP2: a novel functional player in AQP5 trafficking dynamics and implications for Sjögren's disease.
Journal: Cell Commun Signal | Authors: Claudia D'Agostino KO Validated | Species: mouse,human | Application: WB,IF
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