CLK1 Polyclonal antibody 20439-1-AP proteintech
$149.00
In stock
SKU
20439-1-AP
| Catalog Number | 20439-1-AP |
| Synonyms | CDC-like kinase 1, CLK, EC:2.7.12.1, STY |
| Host | Rabbit |
| Reactivity | human |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, IP, ELISA |
| Host / Isotype | Rabbit / IgG |
| Tested Applications — Positive WB detected in | A549 cells, COLO 320 cells, HeLa cells, Jurkat cells, LNCaP cells |
| Tested Applications — Positive IP detected in | COLO 320 cells |
| Recommended Dilutions — Western Blot (WB) | WB : 1:500-1:2000 |
| Recommended Dilutions — Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Positive WB detected in | A549 cells, COLO 320 cells, HeLa cells, Jurkat cells, LNCaP cells |
| Positive IP detected in | COLO 320 cells |
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Tested Reactivity | human |
| Cited Reactivity | human |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CatNo: Ag14214 Product name: Recombinant human CLK1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-143 aa of BC031549 Sequence: MRHSKRTYCPDWDDKDWDYGKWRSSSSHKRRKRSHSSAQENKRCKYNHSKMCDSHYLESRSINEKDYHSRRYIDEYRNDYTQGCEPGHRQRDHESRYQNHSSKSSGRSGRSSYKSKHRIHHSTSHRRSHGKSHRRKRTRSVED Predict reactive species |
| Full Name | CDC-like kinase 1 |
| Calculated Molecular Weight | 484 aa, 57 kDa |
| Observed Molecular Weight | 62 kDa |
| GenBank Accession Number | BC031549 |
| Gene Symbol | CLK1 |
| Gene ID (NCBI) | 1195 |
| RRID | AB_2878686 |
| Conjugate | Unconjugated |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P49759 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| IP protocol for CLK1 antibody 20439-1-AP | Download protocol |
| WB protocol for CLK1 antibody 20439-1-AP | Download protocol |
| human | WB Front Cell Dev Biol CLK1 is a potential tumor suppressor for NSCLC by regulating cell proliferation and immune infiltration. Authors - Rui Ma View Article |
| Citations | 2 |
| Dilutions | WB : 1:500-1:2000 IP : 0.5-4.0 ug for IP and 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate for WB |
| Isotype | IgG |
| Clonality | Polyclonal |
| Clone Number | - |
| Conjugation | Unconjugated |
CLK1 (CDC-like kinase 1) is the dual specificity kinase acting on both serine/threonine and tyrosine-containing substrates. It phosphorylates serine- and arginine-rich (SR) proteins of the spliceosomal complex and may be a constituent of a network of regulatory mechanisms that enable SR proteins to control RNA splicing. It can phosphorylate: SRSF1, SRSF3 and PTPN1 (PMID: 10480872; 19168442). It also regulates the alternative splicing of tissue factor (F3) pre-mRNA in endothelial cells (PMID: 19168442). Elevated CLK1 activity is frequently detected in cancers, where it alters splice isoform balance to promote proliferation, invasion and chemoresistance, making it an emerging therapeutic target. Protocols Product Specific Protocols IP protocol for CLK1 antibody 20439-1-AP Download protocol WB protocol for CLK1 antibody 20439-1-AP Download protocol Standard Protocols Click here to view our Standard Protocols
Publications
- Machine learning-based selection of immune cell markers in osteosarcoma: prognostic determination and validation of CLK1 in disease progression
Journal: Front Immunol | Authors: Nan Zhang | Species: human | Application: WB - CLK1 is a potential tumor suppressor for NSCLC by regulating cell proliferation and immune infiltration.
Journal: Front Cell Dev Biol | Authors: Rui Ma | Species: human | Application: WB
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