CRM1 Monoclonal antibody 66763-1-Ig proteintech
$149.00
In stock
SKU
66763-1-Ig
| Catalog Number | 66763-1-Ig |
| Synonyms | XPO1, 2D3B6, Exp1, Exportin 1, Exportin-1 |
| Host | Mouse |
| Reactivity | human, mouse, rat |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, IHC, IF/ICC, IP, ELISA |
| Applications | WB, IHC, IF/ICC, ELISA |
| Host / Isotype | Mouse / IgG1 |
| Tested Applications — Positive WB detected in | A431 cells, HeLa cells, HepG2 cells, Jurkat cells, K-562 cells, HSC-T6 cells, PC-12 cells, NIH/3T3 cells, RAW 264.7 cells, HEK-293 cells |
| Tested Applications — Positive IHC detected in | human cervical cancer tissue, human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Tested Applications — Positive IF/ICC detected in | K-562 cells |
| Recommended Dilutions — Western Blot (WB) | WB : 1:5000-1:50000 |
| Recommended Dilutions — Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Recommended Dilutions — Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| Positive WB detected in | A431 cells, HeLa cells, HepG2 cells, Jurkat cells, K-562 cells, HSC-T6 cells, PC-12 cells, NIH/3T3 cells, RAW 264.7 cells, HEK-293 cells |
| Positive IHC detected in | human cervical cancer tissue, human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | K-562 cells |
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | CatNo: Ag27337 Product name: Recombinant human XPO1 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 301-540 aa of BC032847 Sequence: NTNIRLAYSNGKDDEQNFIQNLSLFLCTFLKEHDQLIEKRLNLRETLMEALHYMLLVSEVEETEIFKICLEYWNHLAAELYRESPFSTSASPLLSGSQHFDVPPRRQLYLPMLFKVRLLMVSRMAKPEEVLVVENDQGEVVREFMKDTDSINLYKNMRETLVYLTHLDYVDTERIMTEKLHNQVNGTEWSWKNLNTLCWAIGSISGAMHEEDEKRFLVTVIKDLLGLCEQKRGKDNKAII Predict reactive species |
| Full Name | exportin 1 (CRM1 homolog, yeast) |
| Calculated Molecular Weight | 1071 aa, 123 kDa |
| Observed Molecular Weight | 100-110 kDa |
| GenBank Accession Number | BC032847 |
| Gene Symbol | CRM1 |
| Gene ID (NCBI) | 7514 |
| RRID | AB_2882109 |
| Conjugate | Unconjugated |
| Purification Method | Protein G purification |
| UNIPROT ID | O14980 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| IF protocol for CRM1 antibody 66763-1-Ig | Download protocol |
| IHC protocol for CRM1 antibody 66763-1-Ig | Download protocol |
| WB protocol for CRM1 antibody 66763-1-Ig | Download protocol |
| human | IHC PeerJ Hub gene associated with prognosis in bladder cancer is a novel therapeutic target Authors - Dengpan Fang View Article |
| rat,human | WB Diabetologia Saturated fatty acids entrap PDX1 in stress granules and impede islet beta cell function. Authors - Mu Zhang View Article |
| mouse | WB Exp Gerontol Age-dependent changes in nuclear-cytoplasmic signaling in skeletal muscle. Authors - Shama R Iyer View Article |
| Citations | 20 |
| Dilutions | WB : 1:5000-1:50000 IHC : 1:500-1:2000 IF/ICC : 1:400-1:1600 |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Clone Number | 2D3B6 |
| Conjugation | Unconjugated |
CRM1 (chromosome region maintenance 1, also known as exportin-1 or XPO1), a member of the karyopherin β family of transport receptors, is an important nuclear export receptor recognizing proteins bearing a leucine-rich nuclear export signal (PMID: 9323133). CRM1 is the major receptor for the export of proteins out of the nucleus and is also required for transport of many RNAs (PMID: 17317185). In addition to nuclear export, CRM1 also plays a role in centrosome duplication and spindle assembly (PMID: 22773955). Elevated CRM1 expression has been reported in several cancers, which implicates CRM1 as a potential anti-cancer drug target (PMID: 22677130; 21683812 ). Protocols Product Specific Protocols IF protocol for CRM1 antibody 66763-1-Ig Download protocol IHC protocol for CRM1 antibody 66763-1-Ig Download protocol WB protocol for CRM1 antibody 66763-1-Ig Download protocol Standard Protocols Click here to view our Standard Protocols
Publications
- Positive Feedback Regulation between KLF5 and XPO1 Promotes Cell Cycle Progression of Basal like Breast Cancer
Journal: Adv Sci (Weinh) | Authors: Yu Tang KD Validated | Species: human | Application: WB - Saturated fatty acids entrap PDX1 in stress granules and impede islet beta cell function.
Journal: Diabetologia | Authors: Mu Zhang | Species: rat,human | Application: WB - Anti-tumor efficacy of Selinexor (KPT-330) in gastric cancer is dependent on nuclear accumulation of p53 tumor suppressor.
Journal: Sci Rep | Authors: Vinod Vijay Subhash | Application: WB - Age-dependent changes in nuclear-cytoplasmic signaling in skeletal muscle.
Journal: Exp Gerontol | Authors: Shama R Iyer | Species: mouse | Application: WB - NEDD4 protects vascular endothelial cells against Angiotensin II-induced cell death via enhancement of XPO1-mediated nuclear export.
Journal: Exp Cell Res | Authors: Jianning Xu | Species: human | Application: WB - Hub gene associated with prognosis in bladder cancer is a novel therapeutic target
Journal: PeerJ | Authors: Dengpan Fang | Species: human | Application: IHC - Saturated fatty acids entrap PDX1 in stress granules and impede islet beta cell function.
Journal: Diabetologia | Authors: Mu Zhang | Species: rat,human | Application: WB - Mutant p53 upregulates HDAC6 to resist ER stress and facilitates Ku70 deacetylation, which prevents its degradation and mitigates DNA damage in colon cancer cells
Journal: Cell Death Discov | Authors: Rossella Benedetti | Species: human | Application: WB,IP - Control of SOX2 protein stability and tumorigenic activity by E3 ligase CHIP in esophageal cancer cells
Journal: Oncogene | Authors: Li Kang | Species: human | Application: WB - Role of hyperthermia in overcoming HPV‑mediated MHC‑Ⅰ downregulation and promoting immune surveillance.
Journal: Int J Mol Med | Authors: Hanyu Wang - Parylation dependent nuclear export of HMGB1 via exportin 1 contributes to morphine tolerance.
Journal: Brain Behav Immun | Authors: Ying-Ping Liang | Species: rat | Application: WB,IF - Poly (ADP-ribose) polymerase 1 promotes HuR/ELAVL1 cytoplasmic localization and inflammatory gene expression by regulating p38 MAPK activity
Journal: Cell Mol Life Sci | Authors: Xingyue Fu | Species: human | Application: WB - Age-dependent changes in nuclear-cytoplasmic signaling in skeletal muscle.
Journal: Exp Gerontol | Authors: Shama R Iyer | Species: mouse | Application: WB - Anti-tumor efficacy of Selinexor (KPT-330) in gastric cancer is dependent on nuclear accumulation of p53 tumor suppressor.
Journal: Sci Rep | Authors: Vinod Vijay Subhash | Application: WB - Cytoplasmic translocation of tripartite motif-containing 28 is critical for PRRSV-induced autophagy through promoting Vps34-Beclin1 complex formation
Journal: J Virol | Authors: Meng Chen - Nup210 Promotes Colorectal Cancer Progression by Regulating Nuclear Plasma Transport
Journal: Lab Invest | Authors: Fangyi Han | Application: IF - HuR-HuB autoregulatory network governs inflammatory factors expression.
Journal: J Biol Chem | Authors: Xingyue Fu | Species: human, mouse | Application: WB - NEDD4 protects vascular endothelial cells against Angiotensin II-induced cell death via enhancement of XPO1-mediated nuclear export.
Journal: Exp Cell Res | Authors: Jianning Xu | Species: human | Application: WB - Hub gene associated with prognosis in bladder cancer is a novel therapeutic target
Journal: PeerJ | Authors: Dengpan Fang | Species: human | Application: IHC - XPO1 Inhibition enhances sensitivity to platinum-based chemotherapy in germinal-center B-cell-like-DLBCL cells.
Journal: Hematology | Authors: Qiongqiong Su
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