UMP/CMP kinase Polyclonal antibody 11360-1-AP proteintech
$149.00
In stock
SKU
11360-1-AP
| Catalog Number | 11360-1-AP |
| Synonyms | CMPK1, Cytidylate kinase, Cytidine monophosphate kinase, CMPK, CMK |
| Host | Rabbit |
| Reactivity | human, mouse, rat |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, IHC, IF/ICC, ELISA |
| Host / Isotype | Rabbit / IgG |
| Tested Applications — Positive WB detected in | mouse pancreas tissue, human pancreas tissue, human skeletal muscle tissue |
| Tested Applications — Positive IHC detected in | human lung cancer tissue, human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Tested Applications — Positive IF/ICC detected in | HeLa cells |
| Recommended Dilutions — Western Blot (WB) | WB : 1:500-1:1000 |
| Recommended Dilutions — Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Recommended Dilutions — Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Positive WB detected in | mouse pancreas tissue, human pancreas tissue, human skeletal muscle tissue |
| Positive IHC detected in | human lung cancer tissue, human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CatNo: Ag1920 Product name: Recombinant human CMPK1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-228 aa of BC014961 Sequence: MLSRCRSRLLHVLGLSFLLQTRRPILLCSPRLMKPLVVFVLGGPGAGKGTQCARIVEKYGYTHLSAGELLRDERKNPDSQYGELIEKYIKEGKIVPVEITISLLKREMDQTMAANAQKNKFLIDGFPRNQDNLQGWNKTMDGKADVSFVLFFDCNNEICIERCLERGKSSGRSDDNRESLEKRIQTYLQSTKPIIDLYEEMGKVKKIDASKSVDEVFDEVVQIFDKEG Predict reactive species |
| Full Name | cytidine monophosphate (UMP-CMP) kinase 1, cytosolic |
| Calculated Molecular Weight | 228 aa, 26 kDa |
| Observed Molecular Weight | 22-26 kDa |
| GenBank Accession Number | BC014961 |
| Gene Symbol | CMPK1 |
| Gene ID (NCBI) | 51727 |
| RRID | AB_2081395 |
| Conjugate | Unconjugated |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P30085 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| IF protocol for UMP/CMP kinase antibody 11360-1-AP | Download protocol |
| IHC protocol for UMP/CMP kinase antibody 11360-1-AP | Download protocol |
| WB protocol for UMP/CMP kinase antibody 11360-1-AP | Download protocol |
| human | WB ACS Pharmacol Transl Sci All Blood Brain Barrier Cell Types Demonstrate Capability to Influence Differential Tenofovir and Emtricitabine Metabolism and Transport in the Brain Authors - Hannah N Wilkins View Article |
| Citations | 4 |
| Dilutions | WB : 1:500-1:1000 IHC : 1:50-1:500 IF/ICC : 1:50-1:500 |
| Isotype | IgG |
| Clonality | Polyclonal |
| Clone Number | - |
| Conjugation | Unconjugated |
Pyrimidine nucleoside monophosphate kinase [UMP/CMP kinase (UMP/CMPK)] (CMPK1) is also named as CMK, CMPK, UCK, UMK, UMPK and belongs to the adenylate kinase family. It plays a crucial role in the formation of UDP, CDP, and dCDP, which are required for cellular nucleic acid synthesis(PMID:11912132). CMPK1 can use a broad spectrum of phosphate donors but the best phosphate donors are ATP and dATP. CMPK1 has been reported as a prognostic marker for non-small cell lung cancer, pancreatic cancer and breast cancer (PMID: 21642870, 22838950, 27558661). The longest deduced protein contains 228 amino acids and has a calculated molecular mass of 26 kD. However, translation likely begins at the second initiation methionine, resulting in a protein of 196 amino acids(PMID:10462544). The shorter form is the endogenously translated protein(PMID:11912132). CMPK1 has 3 isoforms with the molecular mass of 22, 16 and 26 kDa. Protocols Product Specific Protocols IF protocol for UMP/CMP kinase antibody 11360-1-AP Download protocol IHC protocol for UMP/CMP kinase antibody 11360-1-AP Download protocol WB protocol for UMP/CMP kinase antibody 11360-1-AP Download protocol Standard Protocols Click here to view our Standard Protocols
Publications
- Profiling and identification of new proteins involved in brain ischemia using MALDI-imaging-mass-spectrometry.
Journal: J Proteomics | Authors: Víctor Llombart - Integrated multi-omics profiling identifies Cmpk1 as a monocyte-specific therapeutic target for renal ischemia-reperfusion injury
Journal: Life Sci | Authors: Haoxun Zhang | Application: WB - All Blood Brain Barrier Cell Types Demonstrate Capability to Influence Differential Tenofovir and Emtricitabine Metabolism and Transport in the Brain
Journal: ACS Pharmacol Transl Sci | Authors: Hannah N Wilkins | Species: human | Application: WB - Compartmentalized branched-chain amino acid metabolism orchestrates colorectal cancer dissemination via an UMP-vimentin axis.
Journal: Cell Metab | Authors: Fenfen Ji
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