Annexin A1 Monoclonal antibody 66344-1-Ig proteintech
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66344-1-Ig
| Catalog Number | 66344-1-Ig |
| Synonyms | ANXA1, 1E1B7, Annexin 1, Annexin Ac2-26, Annexin I |
| Host | Mouse |
| Reactivity | human, mouse, rat |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, IHC, IF/ICC, FC (Intra), ELISA |
| Host / Isotype | Mouse / IgG1 |
| Tested Applications — Positive WB detected in | A549 cells, HeLa cells, A431 cells, NIH/3T3 cells, 4T1 cells, HCT 116 cells, Jurkat cells, K-562 cells, HepG2 cells |
| Tested Applications — Positive IHC detected in | human stomach tissue, human liver tissue, human pancreas tissue, mouse liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Tested Applications — Positive IF/ICC detected in | A549 cells |
| Tested Applications — Positive FC (Intra) detected in | K-562 cells |
| Recommended Dilutions — Western Blot (WB) | WB : 1:5000-1:50000 |
| Recommended Dilutions — Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Recommended Dilutions — Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| Recommended Dilutions — Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension |
| Positive WB detected in | A549 cells, HeLa cells, A431 cells, NIH/3T3 cells, 4T1 cells, HCT 116 cells, Jurkat cells, K-562 cells, HepG2 cells |
| Positive IHC detected in | human stomach tissue, human liver tissue, human pancreas tissue, mouse liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | A549 cells |
| Positive FC (Intra) detected in | K-562 cells |
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | CatNo: Ag17273 Product name: Recombinant human ANXA1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-346 aa of BC001275 Sequence: MAMVSEFLKQAWFIENEEQEYVQTVKSSKGGPGSAVSPYPTFNPSSDVAALHKAIMVKGVDEATIIDILTKRNNAQRQQIKAAYLQETGKPLDETLKKALTGHLEEVVLALLKTPAQFDADELRAAMKGLGTDEDTLIEILASRTNKEIRDINRVYREELKRDLAKDITSDTSGDFRNALLSLAKGDRSEDFGVNEDLADSDARALYEAGERRKGTDVNVFNTILTTRSYPQLRRVFQKYTKYSKHDMNKVLDLELKGDIEKCLTAIVKCATSKPAFFAEKLHQAMKGVGTRHKALIRIMVSRSEIDMNDIKAFYQKMYGISLCQAILDETKGDYEKILVALCGGN Predict reactive species |
| Full Name | annexin A1 |
| Calculated Molecular Weight | 346 aa, 39 kDa |
| Observed Molecular Weight | 35 kDa |
| GenBank Accession Number | BC001275 |
| Gene Symbol | Annexin A1 |
| Gene ID (NCBI) | 301 |
| RRID | AB_2881724 |
| Conjugate | Unconjugated |
| Purification Method | Protein A purification |
| UNIPROT ID | P04083 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| FC protocol for Annexin A1 antibody 66344-1-Ig | Download protocol |
| IF protocol for Annexin A1 antibody 66344-1-Ig | Download protocol |
| IHC protocol for Annexin A1 antibody 66344-1-Ig | Download protocol |
| WB protocol for Annexin A1 antibody 66344-1-Ig | Download protocol |
| mouse | WB NPJ Regen Med The SHEDs-derived apoptotic bodies for inflammatory regulation in spinal cord repair. Authors - Lihua Luo View Article |
| human | IF Nat Commun Brain and neural cell type proteomics reveal extracellular matrix proteins enriched in progressive multiple sclerosis. Authors - Henry Wang View Article |
| Citations | 15 |
| Dilutions | WB : 1:5000-1:50000 IHC : 1:50-1:500 IF/ICC : 1:400-1:1600 FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Clone Number | 1E1B7 |
| Conjugation | Unconjugated |
ANXA1, also named as ANX1, LPC1 and p35, belongs to the annexin family. It is a member of the annexin family of Ca2+-binding and phospholipidbinding proteins, and it is particularly abundant in various populations of peripheral blood leukocytes. ANXA1 regulates phospholipase A2 activity. It seems to bind from two to four calcium ions with high affinity. ANXA1 is a pleiotrophic protein produced by many cell types including peripheral blood leucocytes. Protocols Product Specific Protocols FC protocol for Annexin A1 antibody 66344-1-Ig Download protocol IF protocol for Annexin A1 antibody 66344-1-Ig Download protocol IHC protocol for Annexin A1 antibody 66344-1-Ig Download protocol WB protocol for Annexin A1 antibody 66344-1-Ig Download protocol Standard Protocols Click here to view our Standard Protocols
Publications
- M2 Macrophage-Derived Extracellular Vesicles Reprogram Immature Neutrophils into Anxa1hi Neutrophils to Enhance Inflamed Bone Regeneration
Journal: Adv Sci (Weinh) | Authors: Yufei Yao | Species: mouse | Application: IF - Cancer Cell-Derived Large Extracellular Vesicles Promote Venous Thromboembolism by Activating NETosis Through Delivering CYBA
Journal: Adv Sci (Weinh) | Authors: Xiangji Li | Species: human | Application: WB - Proteome-wide and matrisome-specific atlas of the human ovary computes fertility biomarker candidates and open the way for precision oncofertility.
Journal: Matrix Biol | Authors: Emna Ouni | Species: human | Application: IF - Magnetic hyperthermia induces effective and genuine immunogenic tumor cell death with respect to exogenous heating
Journal: J Mater Chem B | Authors: Bin Yan | Species: human | Application: IHC - The SHEDs-derived apoptotic bodies for inflammatory regulation in spinal cord repair.
Journal: NPJ Regen Med | Authors: Lihua Luo | Species: mouse | Application: WB - Brain and neural cell type proteomics reveal extracellular matrix proteins enriched in progressive multiple sclerosis.
Journal: Nat Commun | Authors: Henry Wang | Species: human | Application: IF - From stem cells to nanomedicine: A multimodal approach targeting pancreatic fibrosis via MFGE8-dependent ANXA1-SMAD2/3 axis.
Journal: Int J Biol Macromol | Authors: Wangcheng Xie | Species: human | Application: IF - The SHEDs-derived apoptotic bodies for inflammatory regulation in spinal cord repair.
Journal: NPJ Regen Med | Authors: Lihua Luo | Species: mouse | Application: WB - Multiple Mild Stimulations Reduce Membrane Distribution of CX3CR1 Promoted by Annexin a1 in Microglia to Attenuate Excessive Dendritic Spine Pruning and Cognitive Deficits Caused by a Transient Ischemic Attack in Mice
Journal: Neurosci Bull | Authors: Lu Zheng | Species: mouse | Application: WB,IF - Magnetic hyperthermia induces effective and genuine immunogenic tumor cell death with respect to exogenous heating
Journal: J Mater Chem B | Authors: Bin Yan | Species: human | Application: IHC - Proteome-wide and matrisome-specific atlas of the human ovary computes fertility biomarker candidates and open the way for precision oncofertility.
Journal: Matrix Biol | Authors: Emna Ouni | Species: human | Application: IF - Elevation of ANXA1 associated with potential protective mechanism against ferroptosis and immune cell infiltration in age-related macular degeneration
Journal: Eur J Med Res | Authors: Jing Chen | Species: mouse | Application: IF - Elevation of ANXA1 associated with potential protective mechanism against ferroptosis and immune cell infiltration in age-related macular degeneration
Journal: Eur J Med Res | Authors: Jing Chen | Species: mouse | Application: IF - Effect of Tamoxifen on Proteome Expression during In Vitro Myogenesis in Murine Skeletal Muscle C2C12 Cells
Journal: J Proteome Res | Authors: Emily A Morris | Species: mouse - Annexin A1-Fpr2/ALX signaling in Candida keratitis: Regulatory role in pathogenesis and resolution.
Journal: Exp Eye Res | Authors: Yan Peng
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