CPE Recombinant monoclonal antibody 86111-1-RR proteintech
$149.00
In stock
SKU
86111-1-RR
| Catalog Number | 86111-1-RR |
| Synonyms | Carboxypeptidase E, Carboxypeptidase H, CPH, EC:3.4.17.10, Prohormone-processing carboxypeptidase |
| Host | Rabbit |
| Reactivity | human, mouse, rat |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, IHC, IF/ICC, ELISA |
| Host / Isotype | Rabbit / IgG |
| Tested Applications — Positive WB detected in | PC-12 cells, MCF-7 cells, mouse brain tissue, rat brain tissue |
| Tested Applications — Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Tested Applications — Positive IF/ICC detected in | PC-12 cells |
| Recommended Dilutions — Western Blot (WB) | WB : 1:2000-1:10000 |
| Recommended Dilutions — Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| Recommended Dilutions — Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| Positive WB detected in | PC-12 cells, MCF-7 cells, mouse brain tissue, rat brain tissue |
| Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | PC-12 cells |
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| Tested Reactivity | human, mouse, rat |
| Class | Recombinant |
| Type | Antibody |
| Immunogen | CatNo: Eg2989 Product name: Recombinant Human CPE protein (rFc Tag) Source: mammalian cells -derived, V37 Tag: C-rFc Domain: 26-453 aa of NM_001873.4 Sequence: EAQEPGAPAAGMRRRRRLQQEDGISFEYHRYPELREALVSVWLQCTAISRIYTVGRSFEGRELLVIELSDNPGVHEPGEPEFKYIGNMHGNEAVGRELLIFLAQYLCNEYQKGNETIVNLIHSTRIHIMPSLNPDGFEKAASQPGELKDWFVGRSNAQGIDLNRNFPDLDRIVYVNEKEGGPNNHLLKNMKKIVDQNTKLAPETKAVIHWIMDIPFVLSANLHGGDLVANYPYDETRSGSAHEYSSSPDDAIFQSLARAYSSFNPAMSDPNRPPCRKNDDDSSFVDGTTNGGAWYSVPGGMQDFNYLSSNCFEITVELSCEKFPPEETLKTYWEDNKNSLISYLEQIHRGVKGFVRDLQGNPIANATISVEGIDHDVTSAKDGDYWRLLIPGNYKLTASAPGYLAITKKVAVPYSPAAGVDFELESFS Predict reactive species |
| Full Name | carboxypeptidase E |
| Calculated Molecular Weight | 53 kDa |
| Observed Molecular Weight | 50-55 kDa |
| GenBank Accession Number | NM_001873.4 |
| Gene Symbol | Carboxypeptidase E |
| Gene ID (NCBI) | 1363 |
| RRID | AB_3744629 |
| Conjugate | Unconjugated |
| Purification Method | Protein A purification |
| UNIPROT ID | P16870-1 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| IF protocol for CPE antibody 86111-1-RR | Download protocol |
| IHC protocol for CPE antibody 86111-1-RR | Download protocol |
| WB protocol for CPE antibody 86111-1-RR | Download protocol |
| Citations | - |
| Dilutions | WB : 1:2000-1:10000 IHC : 1:250-1:1000 IF/ICC : 1:200-1:800 |
| Isotype | IgG |
| Clonality | Recombinant |
| Clone Number | 250680F12 |
| Conjugation | Unconjugated |
CPE(Carboxypeptidase E) is also named as CPH.It belongs to the peptidase M14 family and is involved in the biosynthesis of peptide hormones and neurotransmitters, including insulin.The deduced 476-amino acid human protein contains an N-terminal signal peptide, followed by a 'pro' sequence, a polyarginine stretch, and 2 potential N-glycosylation sites. Protocols Product Specific Protocols IF protocol for CPE antibody 86111-1-RR Download protocol IHC protocol for CPE antibody 86111-1-RR Download protocol WB protocol for CPE antibody 86111-1-RR Download protocol Standard Protocols Click here to view our Standard Protocols
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