UPF1 Monoclonal antibody 66898-1-Ig proteintech
$149.00
In stock
SKU
66898-1-Ig
| Catalog Number | 66898-1-Ig |
| Synonyms | RENT1, Regulator of nonsense transcripts 1, KIAA0221, EC:3.6.4.12, ATP-dependent helicase RENT1 |
| Host | Mouse |
| Reactivity | human, mouse, rat |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, IHC, IF/ICC, FC (Intra), RIP, ELISA |
| Applications | WB, IHC, IF/ICC, FC (Intra), ELISA |
| Host / Isotype | Mouse / IgG1 |
| Tested Applications — Positive WB detected in | HEK-293 cells, mouse testis tissue, HeLa cells, HepG2 cells, Raji cells, Jurkat cells, HSC-T6 cells, NIH/3T3 cells, mouse brain tissue |
| Tested Applications — Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Tested Applications — Positive IF/ICC detected in | HepG2 cells |
| Tested Applications — Positive FC (Intra) detected in | HepG2 cells |
| Recommended Dilutions — Western Blot (WB) | WB : 1:3000-1:10000 |
| Recommended Dilutions — Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| Recommended Dilutions — Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| Recommended Dilutions — Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| Positive WB detected in | HEK-293 cells, mouse testis tissue, HeLa cells, HepG2 cells, Raji cells, Jurkat cells, HSC-T6 cells, NIH/3T3 cells, mouse brain tissue |
| Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
| Positive FC (Intra) detected in | HepG2 cells |
| Western Blot (WB) | WB : 1:3000-1:10000 |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | CatNo: Ag28320 Product name: Recombinant human UPF1 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 769-1118 aa of BC039817 Sequence: EAANVEKITTKLLKAGAKPDQIGIITPYEGQRSYLVQYMQFSGSLHTKLYQEVEIASVDAFQGREKDFIILSCVRANEHQGIGFLNDPRRLNVALTRARYGVIIVGNPKALSKQPLWNHLLNYYKEQKVLVEGPLNNLRESLMQFSKPRKLVNTINPGARFMTTAMYDAREAIIPGSVYDRSSQGRPSSMYFQTHDQIGMISAGPSHVAAMNIPIPFNLVMPPMPPPGYFGQANGPAAGRGTPKGKTGRGGRQKNRFGLPGPSQTNLPNSQASQDVASQPFSQGALTQGYISMSQPSQMSQPGLSQPELSQDSYLGDEFKSQIDVALSQDSTYQGERAYQHGGVTGLSQY Predict reactive species |
| Full Name | UPF1 regulator of nonsense transcripts homolog (yeast) |
| Calculated Molecular Weight | 123 kDa |
| Observed Molecular Weight | 123-130 kDa |
| GenBank Accession Number | BC039817 |
| Gene Symbol | UPF1 |
| Gene ID (NCBI) | 5976 |
| RRID | AB_2882227 |
| Conjugate | Unconjugated |
| Purification Method | Protein G purification |
| UNIPROT ID | Q92900 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| FC protocol for UPF1 antibody 66898-1-Ig | Download protocol |
| IF protocol for UPF1 antibody 66898-1-Ig | Download protocol |
| IHC protocol for UPF1 antibody 66898-1-Ig | Download protocol |
| WB protocol for UPF1 antibody 66898-1-Ig | Download protocol |
| mouse | WB J Neurosci Inhibition of Nonsense-Mediated Decay Induces Nociceptive Sensitization through Activation of the Integrated Stress Response Authors - June Bryan de la Peña View Article |
| human | WB Cell Signal Exosomal circ_0097112 drives icotinib resistance through activating RAF/MAPK signaling in lung adenocarcinoma. Authors - Ziyuan Chen View Article |
| BARBOSA (Verified Customer) (01-30-2026) | Good Applications: Western Blot Cell Tissue Type: HEK |
| Citations | 15 |
| Dilutions | WB : 1:3000-1:10000 IHC : 1:250-1:1000 IF/ICC : 1:400-1:1600 FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Clone Number | 3B6B5 |
| Conjugation | Unconjugated |
Up-Frameshift Suppressor 1 Homolog (UPF1) is the central factor in nonsense-mediated mRNA decay (NMD) and is also directly involved in telomere homeostasis, DNA replication, histone mRNA degradation and staufen-mediated mRNA decay (PMID: 29382845). It is a potential modulator of MALAT1 and that UPF1/MALAT1 pathway could be a therapeutic target for gastric cancer (PMID: 28942451). The molecular mass of UPF1 is 123-130 kDa. Protocols Product Specific Protocols FC protocol for UPF1 antibody 66898-1-Ig Download protocol IF protocol for UPF1 antibody 66898-1-Ig Download protocol IHC protocol for UPF1 antibody 66898-1-Ig Download protocol WB protocol for UPF1 antibody 66898-1-Ig Download protocol Standard Protocols Click here to view our Standard Protocols
Publications
- Inhibition of Nonsense-Mediated Decay Induces Nociceptive Sensitization through Activation of the Integrated Stress Response
Journal: J Neurosci | Authors: June Bryan de la Peña | Species: mouse | Application: WB - Prenatal diagnostic approaches diagnosed craniosynostosis and identified a novel nonsense variant in SMAD6 in a Chinese fetus
Journal: Gene | Authors: Zhidan Hong | Species: human | Application: WB - LINC00626 drives tamoxifen resistance in breast cancer cells by interaction with UPF1
Journal: Sci Rep | Authors: Hui Yuan | Species: human | Application: WB,RIP - Novel PKP2 compound heterozygous mutations causing neonatal early-onset arrhythmogenic cardiomyopathy: insights into the synergistic pathogenicity of biallelic inactivation.
Journal: Funct Integr Genomics | Authors: Xiaobin Li | Species: human | Application: WB - Exosomal circ_0097112 drives icotinib resistance through activating RAF/MAPK signaling in lung adenocarcinoma.
Journal: Cell Signal | Authors: Ziyuan Chen | Species: human | Application: WB - Genome-Wide Impact of Human DBR1 Depletion on RNA Processing Networks Reveal a Connection Between Pre-mRNA Splicing, mRNA Surveillance and Stress Granule Dynamics.
Journal: J Mol Biol | Authors: Tiffany Barwell
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