| Catalog Number | 13057-2-AP |
| Synonyms | ATP-dependent DNA helicase VIII, EC:3.6.4.12, G3BP, G3BP 1, G3BP-1 |
| Host | Rabbit |
| Reactivity | human, mouse, rat and More (5) |
| Reactivity | human, mouse, rat, pig, monkey, chicken, zebrafish, drosophila melanogaster (fruit fly) |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, IHC, IF/ICC, FC (Intra), IP, CoIP, RIP, ELISA |
| Applications | WB, IHC, IF/ICC, FC (Intra), IP, ELISA |
| Host / Isotype | Rabbit / IgG |
| Tested Applications — Positive WB detected in | C6 cells, HEK-293 cells, human brain tissue, Neuro-2a cells, Jurkat cells, MCF-7 cells, HeLa cells, mouse kidney tissue, rat kidney tissue, mouse brain tissue, rat brain tissue |
| Tested Applications — Positive IP detected in | HEK-293 cells |
| Tested Applications — Positive IHC detected in | human lung cancer tissue, human breast cancer tissue, human colorectal cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Tested Applications — Positive IF/ICC detected in | sodium arsenite treated HeLa cells |
| Tested Applications — Positive FC (Intra) detected in | HeLa cells |
| Recommended Dilutions — Western Blot (WB) | WB : 1:2000-1:16000 |
| Recommended Dilutions — Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Recommended Dilutions — Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Recommended Dilutions — Immunofluorescence (IF)/ICC | IF/ICC : 1:1000-1:4000 |
| Recommended Dilutions — Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
| Positive WB detected in | C6 cells, HEK-293 cells, human brain tissue, Neuro-2a cells, Jurkat cells, MCF-7 cells, HeLa cells, mouse kidney tissue, rat kidney tissue, mouse brain tissue, rat brain tissue |
| Positive IP detected in | HEK-293 cells |
| Positive IHC detected in | human lung cancer tissue, human breast cancer tissue, human colorectal cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | sodium arsenite treated HeLa cells |
| Positive FC (Intra) detected in | HeLa cells |
| Western Blot (WB) | WB : 1:2000-1:16000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:1000-1:4000 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, pig, monkey, chicken, zebrafish, drosophila melanogaster (fruit fly) |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CatNo: Ag3728 Product name: Recombinant human G3BP1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 167-466 aa of BC006997 Sequence: PDDSGTFYDQAVVSNDMEEHLEEPVAEPEPDPEPEPEQEPVSEIQEEKPEPVLEETAPEDAQKSSSPAPADIAQTVQEDLRTFSWASVTSKNLPPSGAVPVTGIPPHVVKVPASQPRPESKPESQIPPQRPQRDQRVREQRINIPPQRGPRPIREAGEQGDIEPRRMVRHPDSHQLFIGNLPHEVDKSELKDFFQSYGNVVELRINSGGKLPNFGFVVFDDSEPVQKVLSNRPIMFRGEVRLNVEEKKTRAAREGDRRDNRLRGPGGPRGGLGGGMRGPPRGGMVQKPGFGVGRGLAPRQ Predict reactive species |
| Full Name | GTPase activating protein (SH3 domain) binding protein 1 |
| Calculated Molecular Weight | 466 aa, 52 kDa |
| Observed Molecular Weight | 68 kDa |
| GenBank Accession Number | BC006997 |
| Gene Symbol | G3BP1 |
| Gene ID (NCBI) | 10146 |
| RRID | AB_2232034 |
| Conjugate | Unconjugated |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q13283 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| FC protocol for G3BP1 antibody 13057-2-AP | Download protocol |
| IF protocol for G3BP1 antibody 13057-2-AP | Download protocol |
| IHC protocol for G3BP1 antibody 13057-2-AP | Download protocol |
| IP protocol for G3BP1 antibody 13057-2-AP | Download protocol |
| WB protocol for G3BP1 antibody 13057-2-AP | Download protocol |
| mouse,human,chicken | IF,CoIP Cell Diverse CMT2 neuropathies are linked to aberrant G3BP interactions in stress granules Authors - Qinqin Cui View Article |
| human | IF Cell Phase Separation of FUS Is Suppressed by Its Nuclear Import Receptor and Arginine Methylation. Authors - Mario Hofweber View Article |
| Vasudevarao (Verified Customer) (04-09-2026) | Antibody works well for western blot in 1:1000 dilution, incubated overnight in 3%BSA/PBS on human lung cancer cell lines. Applications: Western Blot, Immunofluorescence Primary Antibody Dilution: 1:1000 Cell Tissue Type: Prostate cancer cells |
| Prakash (Verified Customer) (10-17-2025) | working very well in western blot Applications: Western Blot Primary Antibody Dilution: 1:4000 for western Cell Tissue Type: T24 Bladder cancer cell |
| lu (Verified Customer) (09-18-2025) | Good antibody for flow cytometry Applications: Flow Cytometry Primary Antibody Dilution: 1:1000 Cell Tissue Type: OPM2 |
| Nikolett (Verified Customer) (07-29-2025) | Antibody works well for western blot in 1:1000 dilution, incubated overnight in 3%BSA/PBS on human lung cancer cell lines. Applications: Western Blot Primary Antibody Dilution: 1:1000 Cell Tissue Type: Human Lung Cancer Patient Derived Cell Lines |
| Xiaochen (Verified Customer) (11-11-2024) | Applications: Immunofluorescence Cell Tissue Type: SH-SY5Y G3BP1 Antibody Immunofluorescence validation ( dilution) in SH-SY5Y (Cat no:13057-2-AP) |
| Roy (Verified Customer) (06-12-2024) | Works great on WB (1/1000 - Overnight 4°C) - Very beautiful IF staining in non stressed (diffuse staining) and Sodium Arsenite-mediated Stress induction (Punctate staining corresponding to stress granules) in HeLa cells (1/250 - 1h at RT). Applications: Western Blot, Immunofluorescence Primary Antibody Dilution: 1/1000 Cell Tissue Type: HeLa |
| Vinny (Verified Customer) (02-20-2024) | Good product. Applications: Immunofluorescence |
| Andrea (Verified Customer) (10-05-2023) | Good and strong signal. Applications: Western Blot |
| Tatyana (Verified Customer) (01-21-2023) | Used for IP and WB of GFP-tagged overexpressed human G3BP1 (HEK293 cells). Lysates were subjected to IP using GFP-Trap beads. WB was done using semi-dry transfer. Antibody was used in 4% milk in TBST at 1:1,000 dilution. It could be reused up to 3 times. As the image shows, the antibody can successfully detect both endogenous and OE protein. Applications: Immunofluorescence, Immunoprecipitation Primary Antibody Dilution: 1:1000 Cell Tissue Type: HEK293 G3BP1 Antibody Immunofluorescence,Immunoprecipitation validation (1:1000 dilution) in HEK293 (Cat no:13057-2-AP) |
| Tobias (Verified Customer) (10-25-2022) | Applications: Western Blot |
| Peter (Verified Customer) (10-24-2022) | Best G3BP1 antibody I've used for Western, IF and IP Applications: Western Blot, Immunofluorescence, Immunoprecipitation Primary Antibody Dilution: 1:1000 for WB. 1:200 for IF Cell Tissue Type: A549 and U2OS |
| Patryk (Verified Customer) (03-18-2021) | I used the antibody for immunofluorescence imaging to label stress granules induced by treating the cells with with 50µM sodium arsenite. Used the antibody at a dilution 1:100 overnight at 4°C. Worked perfectly well, strong and specific signal. I am very satisfied of this antibody and strongly recommend if for immunofluorescence. Applications: Immunofluorescence Primary Antibody Dilution: 1:100 Cell Tissue Type: HCT116 cells G3BP1 Antibody Immunofluorescence validation (1:100 dilution) in HCT116 cells (Cat no:13057-2-AP) |
| Kun (Verified Customer) (03-23-2020) | Very specific and sensitive Applications: Western Blot Primary Antibody Dilution: 1:1000 |
| Biao (Verified Customer) (03-11-2020) | This antibody is very specific and good quality. Applications: Western Blot, Cell Tissue Type: hct-116 |
| Joshua (Verified Customer) (12-28-2019) | PANC1 cells fixed in 4% paraformaldehdye. Bright localization to stress granules. Applications: Immunofluorescence, Primary Antibody Dilution: 1:500 Cell Tissue Type: human pancreatic adenocarcinoma cells G3BP1 Antibody Immunofluorescence, validation (1:500 dilution) in human pancreatic adenocarcinoma cells (Cat no:13057-2-AP) |
| Yuan (Verified Customer) (11-02-2019) | Very bright staining for stress granule on NaAsO2 treated Hela cells. 1:500 should be sufficient for IF staining. Applications: Immunofluorescence, Primary Antibody Dilution: 1:250 Cell Tissue Type: Hela G3BP1 Antibody Immunofluorescence, validation (1:250 dilution) in Hela (Cat no:13057-2-AP) |
| Zeinab (Verified Customer) (08-19-2019) | It worked great Applications: Immunofluorescence, Primary Antibody Dilution: 1:400 |
| Erica (Verified Customer) (05-15-2019) | Our lab has been using this antibody for IP, WB and IF for many years and it always worked well. I highly recommend this antibody, especially for IP for stress granules. Applications: Western Blot, Immunofluorescence, Immunoprecipitation, Cell Tissue Type: HeLa; MEF |
| Karthik (Verified Customer) (04-24-2019) | Upon induction of sodium arsenite stress in neurons G3BP1 positive stress granules formed in 90 minutes.Cells permeabilized with 0.2% triton for 10 minutes Applications: Immunofluorescence, Primary Antibody Dilution: 1: 500 overnight at 4 degrees Cell Tissue Type: Primary motor neurons G3BP1 Antibody Immunofluorescence, validation (1: 500 overnight at 4 degrees dilution) in Primary motor neurons (Cat no:13057-2-AP) |
| Tian (Verified Customer) (01-23-2019) | I used it for ICC and it worked Great. Applications: Immunofluorescence, Primary Antibody Dilution: 1;100 Cell Tissue Type: NIH3T3 |
| Citations | 291 |
| Dilutions | WB : 1:2000-1:16000 IP : 0.5-4.0 ug for IP and 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate for WB IHC : 1:50-1:500 IF/ICC : 1:1000-1:4000 FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
| Isotype | IgG |
| Clonality | Polyclonal |
| Clone Number | - |
| Conjugation | Unconjugated |
GAP SH3 Binding Protein 1 (G3BP1), also named as G3BP, is an effector of stress granule (SG) assembly. SG biology plays an important role in the pathophysiology of TDP-43 in ALS and FTLD-U. G3BP1 can be used as a marker of SG. It has been shown to function downstream of Ras and play a role in RNA metabolism, signal transduction, and proliferation. G3BP1 is a ubiquitously expressed protein that localizes to the cytoplasm in proliferating cells and to the nucleus in non-proliferating cells. G3BP1 has recently been implicated in cancer biology. Protocols Product Specific Protocols FC protocol for G3BP1 antibody 13057-2-AP Download protocol IF protocol for G3BP1 antibody 13057-2-AP Download protocol IHC protocol for G3BP1 antibody 13057-2-AP Download protocol IP protocol for G3BP1 antibody 13057-2-AP Download protocol WB protocol for G3BP1 antibody 13057-2-AP Download protocol Standard Protocols Click here to view our Standard Protocols
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