GRP94 Monoclonal antibody 60012-2-Ig proteintech
$149.00
In stock
SKU
60012-2-Ig
| Catalog Number | 60012-2-Ig |
| Synonyms | HSP90B1, 4G7C7, 94 kDa glucose-regulated protein, ECGP, Endoplasmin |
| Host | Mouse |
| Reactivity | human |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, IHC, IF/ICC, FC (Intra), ELISA |
| Host / Isotype | Mouse / IgG1 |
| Tested Applications — Positive WB detected in | HeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells |
| Tested Applications — Positive IHC detected in | human colon cancer tissue, human pancreas cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Tested Applications — Positive IF/ICC detected in | HepG2 cells, A431 cells |
| Tested Applications — Positive FC (Intra) detected in | HeLa cells |
| Recommended Dilutions — Western Blot (WB) | WB : 1:2000-1:10000 |
| Recommended Dilutions — Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| Recommended Dilutions — Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| Recommended Dilutions — Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| Positive WB detected in | HeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells |
| Positive IHC detected in | human colon cancer tissue, human pancreas cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells, A431 cells |
| Positive FC (Intra) detected in | HeLa cells |
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| Tested Reactivity | human |
| Cited Reactivity | human |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | CatNo: Ag1439 Product name: Recombinant human GRP94 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-315 aa of BC009195 Sequence: MRALWVLGLCCVLLTFGSVRADDEVDVDGTVEEDLGKSREGSRTDDEVVQREEEAIQLDGLNASQIRELREKSEKFAFQAEVNRMMKLIINSLYKNKEIFLRELISNASDALDKIRLISLTDENALSGNEELTVKIKCDKEKNLLHVTDTGVGMTREELVKNLGTIAKSGTSEFLNKMTEAQEDGQSTSELIGQFGVGFYSAFLVADKVIVTSKHNNDTQHIWESDSNEFSVIADPRGNTLGRGTTITLVLKEEASDYLELDTIKNLVKKYSQFINFPIYVWSSKTETVEEPMEEEEAAKEEKEESDDEAAARRR Predict reactive species |
| Full Name | heat shock protein 90kDa beta (Grp94), member 1 |
| Calculated Molecular Weight | 96 kDa |
| Observed Molecular Weight | 95 kDa |
| GenBank Accession Number | BC009195 |
| Gene Symbol | GRP94 |
| Gene ID (NCBI) | 7184 |
| RRID | AB_2881351 |
| Conjugate | Unconjugated |
| Purification Method | Protein G purification |
| UNIPROT ID | P14625 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| FC protocol for GRP94 antibody 60012-2-Ig | Download protocol |
| IF protocol for GRP94 antibody 60012-2-Ig | Download protocol |
| IHC protocol for GRP94 antibody 60012-2-Ig | Download protocol |
| WB protocol for GRP94 antibody 60012-2-Ig | Download protocol |
| human | WB J Transl Med Comprehensive profiling of extracellular vesicles in uveitis and scleritis enables biomarker discovery and mechanism exploration Authors - Lingzi Wu View Article |
| Udesh (Verified Customer) (08-19-2024) | Worked well at 1:2000 Applications: Western Blot Primary Antibody Dilution: 1:2000 Cell Tissue Type: MSCs GRP94 Antibody Western Blot validation (1:2000 dilution) in MSCs (Cat no:60012-2-Ig) |
| Citations | 12 |
| Dilutions | WB : 1:2000-1:10000 IHC : 1:200-1:800 IF/ICC : 1:400-1:1600 FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Clone Number | 4G7C7 |
| Conjugation | Unconjugated |
Glucose-regulated protein 94 (GRP94), also called HSP90B1 and GP96, is an endoplasmic reticulum (ER)-resident member of the heat shock protein 90 (HSP90) family (PMID: 33802964; 24658275). Under ER stress conditions, GRP94 accelerates its function as a molecular chaperone. Client proteins of GRP94 include toll-like receptors (TLRs), glycoprotein (GP) IX subunit, insulin-like growth factors (IGFs), proinsulin, and integrins (PMID: 7913987; 32781621; 22079671). As well as being an ER chaperone, GRP94 can also participate in Calcium regulation and is also a regulator of innate and adaptive immunity (PMID: 33802964; 11584270). Protocols Product Specific Protocols FC protocol for GRP94 antibody 60012-2-Ig Download protocol IF protocol for GRP94 antibody 60012-2-Ig Download protocol IHC protocol for GRP94 antibody 60012-2-Ig Download protocol WB protocol for GRP94 antibody 60012-2-Ig Download protocol Standard Protocols Click here to view our Standard Protocols
Publications
- Sertoli cell-derived extracellular vesicles traverse the blood-testis barrier and deliver miR-24-3p inhibitor into germ cells improving sperm mobility
Journal: J Control Release | Authors: Yabing Chen | Application: WB - Berberine inhibits proliferation and migration of colorectal cancer cells by downregulation of GRP78.
Journal: Anticancer Drugs | Authors: Chenxue Gong | Species: human | Application: WB - Exogenous spermidine alleviates diabetic cardiomyopathy via suppressing reactive oxygen species, endoplasmic reticulum stress, and Pannexin-1-mediated ferroptosis
Journal: Biomol Biomed | Authors: Jian Sun | Application: WB - DNAJC10 maintains survival and self-renewal of leukemia stem cells through PERK branch of the unfolded protein response
Journal: Haematologica | Authors: Minjing Li - CKAP4 in Extracellular Vesicle-Derived From Podocyte Serves as a Non-Invasive Diagnostic Biomarker for Diabetic Nephropathy and Promotes Vascular Calcification.
Journal: J Extracell Vesicles | Authors: Heng Zhang | Species: human | Application: WB - Comprehensive profiling of extracellular vesicles in uveitis and scleritis enables biomarker discovery and mechanism exploration
Journal: J Transl Med | Authors: Lingzi Wu | Species: human | Application: WB - Alpha-hederin reprograms multi-miRNAs activity and overcome small extracellular vesicles-mediated paclitaxel resistance in NSCLC
Journal: Front Pharmacol | Authors: Yuzhen Chang | Species: human | Application: WB - Hsp90 β is Critical for the Infection of Severe Fever with Thrombocytopenia Syndrome Virus
Journal: Virol Sin | Authors: Bo Wang | Species: human | Application: WB - Disease-associated TNFRSF11B variants differentially disrupt osteoprotegerin secretion and function.
Journal: Bone | Authors: Reina Suzuki | Application: IF - A New Histology-Based Prognostic Index for Acute Lymphoblastic Leukemia: Preliminary Results of the "ALL Urayasu Classification".
Journal: J Clin Med | Authors: Toru Mitsumori | Species: human | Application: IHC - Exogenous spermidine alleviates diabetic cardiomyopathy via suppressing reactive oxygen species, endoplasmic reticulum stress, and Pannexin-1-mediated ferroptosis
Journal: Biomol Biomed | Authors: Jian Sun | Application: WB - Berberine inhibits proliferation and migration of colorectal cancer cells by downregulation of GRP78.
Journal: Anticancer Drugs | Authors: Chenxue Gong | Species: human | Application: WB
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