TAK1 Monoclonal antibody 67707-1-Ig proteintech
$149.00
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SKU
67707-1-Ig
| Catalog Number | 67707-1-Ig |
| Synonyms | MAP3K7, 1A5B2, EC:2.7.11.25, MAP3K 7, mitogen activated protein kinase kinase kinase 7 |
| Host | Mouse |
| Reactivity | human, mouse, rat |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, IHC, IF/ICC, FC (Intra), IP, ELISA |
| Applications | WB, IHC, IF/ICC, FC (Intra), ELISA |
| Host / Isotype | Mouse / IgG1 |
| Tested Applications — Positive WB detected in | A431 cells, NIH/3T3 cells, HepG2 cells, A549 cells, HEK-293 cells, Jurkat cells, K-562 cells, HSC-T6 cells, RAW 264.7 cells |
| Tested Applications — Positive IHC detected in | human prostate cancer tissue, mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Tested Applications — Positive IF/ICC detected in | HepG2 cells |
| Tested Applications — Positive FC (Intra) detected in | K-562 cells |
| Recommended Dilutions — Western Blot (WB) | WB : 1:500-1:3000 |
| Recommended Dilutions — Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| Recommended Dilutions — Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Recommended Dilutions — Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension |
| Positive WB detected in | A431 cells, NIH/3T3 cells, HepG2 cells, A549 cells, HEK-293 cells, Jurkat cells, K-562 cells, HSC-T6 cells, RAW 264.7 cells |
| Positive IHC detected in | human prostate cancer tissue, mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
| Positive FC (Intra) detected in | K-562 cells |
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | CatNo: Ag21286 Product name: Recombinant human MAP3K7 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 1-291 aa of BC017715 Sequence: MSTASAASSSSSSSAGEMIEAPSQVLNFEEIDYKEIEVEEVVGRGAFGVVCKAKWRAKDVAIKQIESESERKAFIVELRQLSRVNHPNIVKLYGACLNPVCLVMEYAEGGSLYNVLHGAEPLPYYTAAHAMSWCLQCSQGVAYLHSMQPKALIHRDLKPPNLLLVAGGTVLKICDFGTACDIQTHMTNNKGSAAWMAPEVFEGSNYSEKCDVFSWGIILWEVITRRKPFDEIGGPAFRIMWAVHNGTRPPLIKNLPKPIESLMTRCWSKDPSQRPSMEEIVKIMTHLMRYF Predict reactive species |
| Full Name | mitogen-activated protein kinase kinase kinase 7 |
| Calculated Molecular Weight | 579 aa, 64 kDa |
| Observed Molecular Weight | 67-70 kDa |
| GenBank Accession Number | BC017715 |
| Gene Symbol | TAK1 |
| Gene ID (NCBI) | 6885 |
| RRID | AB_2882898 |
| Conjugate | Unconjugated |
| Purification Method | Protein G purification |
| UNIPROT ID | O43318 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| IF protocol for TAK1 antibody 67707-1-Ig | Download protocol |
| IHC protocol for TAK1 antibody 67707-1-Ig | Download protocol |
| WB protocol for TAK1 antibody 67707-1-Ig | Download protocol |
| human | WB Biochim Biophys Acta Mol Cell Res IL-17 promotes melanoma through TRAF2 as a scaffold protein recruiting PIAS2 and ELAVL1 to induce EPHA5 Authors - Junfeng Du View Article |
| mouse | IF Exp Cell Res Deubiquitinase USP18 inhibits hepatic stellate cells activation and alleviates liver fibrosis via regulation of TAK1 activity Authors - Yan Zhang View Article |
| rat | IF Neurochem Res Exogenous TIPE2 Inhibit TAK1 to Improve Inflammation and Neuropathic Pain Induced by Sciatic Nerve Injury Through Inactivating NF-κB and JNK. Authors - Xuehua Sun View Article |
| Citations | 17 |
| Dilutions | WB : 1:500-1:3000 IHC : 1:250-1:1000 IF/ICC : 1:50-1:500 FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Clone Number | 1A5B2 |
| Conjugation | Unconjugated |
MAP3K7(Mitogen-activated protein kinase kinase kinase 7) is also named as TAK1 and belongs to the MAP kinase kinase kinase subfamily. It plays an important role in the cascades of cellular responses evoked by changes in the environment. It has been linked to interleukin-1 receptor and tumor necrosis factor receptor signaling (PMID: 16186825). It has 4 isoforms (53-55 kDa, 64-70 kDa and 75-80 kDa) produced by alternative splicing. Protocols Product Specific Protocols IF protocol for TAK1 antibody 67707-1-Ig Download protocol IHC protocol for TAK1 antibody 67707-1-Ig Download protocol WB protocol for TAK1 antibody 67707-1-Ig Download protocol Standard Protocols Click here to view our Standard Protocols
Publications
- USP4 promotes the proliferation, migration, and invasion of esophageal squamous cell carcinoma by targeting TAK1
Journal: Cell Death Dis | Authors: Hongdian Zhang | Species: human | Application: IHC - Liensinine reduces acute lung injury brought on by lipopolysaccharide by inhibiting the activation of the NF-κB signaling pathway through modification of the Src/TRAF6/TAK1 axis
Journal: Inflammopharmacology | Authors: Huizhen Chen | Species: mouse | Application: WB,IP - Kongensin a attenuates intervertebral disc degeneration by inhibiting TAK1-mediated PANoptosis of nucleus pulposus cells
Journal: Int Immunopharmacol | Authors: Yangyang Chen | Species: rat | Application: WB,IHC - IL-17 promotes melanoma through TRAF2 as a scaffold protein recruiting PIAS2 and ELAVL1 to induce EPHA5
Journal: Biochim Biophys Acta Mol Cell Res | Authors: Junfeng Du | Species: human | Application: WB - Deubiquitinase USP18 inhibits hepatic stellate cells activation and alleviates liver fibrosis via regulation of TAK1 activity
Journal: Exp Cell Res | Authors: Yan Zhang | Species: mouse | Application: IF - Exogenous TIPE2 Inhibit TAK1 to Improve Inflammation and Neuropathic Pain Induced by Sciatic Nerve Injury Through Inactivating NF-κB and JNK.
Journal: Neurochem Res | Authors: Xuehua Sun | Species: rat | Application: IF - Liensinine reduces acute lung injury brought on by lipopolysaccharide by inhibiting the activation of the NF-κB signaling pathway through modification of the Src/TRAF6/TAK1 axis
Journal: Inflammopharmacology | Authors: Huizhen Chen | Species: mouse | Application: WB,IP - Kongensin a attenuates intervertebral disc degeneration by inhibiting TAK1-mediated PANoptosis of nucleus pulposus cells
Journal: Int Immunopharmacol | Authors: Yangyang Chen | Species: rat | Application: WB,IHC - The role of TRAF6 in regulating MAP3K7/P38/MAPK pathway in the epithelial-mesenchymal transition of retinal pigment epithelial cells in proliferative vitreoretinopathy.
Journal: Cell Signal | Authors: Zhen Chen - TRIM44 aggravates cardiac fibrosis after myocardial infarction via TAK1 stabilization
Journal: Cell Signal | Authors: Hao Qian | Species: rat | Application: IF - Galangin Alleviates Helicobacter pylori Gastritis Through TLR2 Inhibition and Modulation of the MyD88-Dependent Inflammatory Cascades.
Journal: Helicobacter | Authors: Wuyinxiao Zheng | Species: human | Application: WB - Exogenous TIPE2 Inhibit TAK1 to Improve Inflammation and Neuropathic Pain Induced by Sciatic Nerve Injury Through Inactivating NF-κB and JNK.
Journal: Neurochem Res | Authors: Xuehua Sun | Species: rat | Application: IF - IL-17 promotes melanoma through TRAF2 as a scaffold protein recruiting PIAS2 and ELAVL1 to induce EPHA5
Journal: Biochim Biophys Acta Mol Cell Res | Authors: Junfeng Du | Species: human | Application: WB - Deubiquitinase USP18 inhibits hepatic stellate cells activation and alleviates liver fibrosis via regulation of TAK1 activity
Journal: Exp Cell Res | Authors: Yan Zhang | Species: mouse | Application: IF - Gentiana szechenyii Kanitz Compounds Regulate Lipopolysaccharide-Induced Inflammation and Mitochondrial Autophagy Via the Toll-Like Receptor 4-Nuclear Factor Kappa B Pathway.
Journal: Chem Biodivers | Authors: Haihua Zhang | Species: mouse | Application: WB - YEATS2/TAK1 axis mediates TGF-β1 driven adaptive resistance to sorafenib in hepatocellular carcinoma.
Journal: Biochem Biophys Res Commun | Authors: Lizhi Bai | Species: human | Application: IF - Chinese medicine formula "Qingxuan Runmu Yin" alleviating ocular surface inflammation in a rat model of dry eye disease by modulating the TLR4/TAK1/p38MAPK pathway.
Journal: Int J Ophthalmol | Authors: Ying Liu | Species: rat | Application: WB
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