Alpha Actinin Monoclonal antibody 66895-1-Ig proteintech
$149.00
In stock
SKU
66895-1-Ig
| Catalog Number | 66895-1-Ig |
| Synonyms | ACTN1, Alpha actinin 1, 1E2E1, actin, actinin, alpha 1 |
| Host | Mouse |
| Reactivity | human, mouse, rat, pig, rabbit and More (1) |
| Reactivity | human, mouse, rat, pig, rabbit, mosquito |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, IHC, IF/ICC, FC (Intra), ELISA |
| Host / Isotype | Mouse / IgG2b |
| Tested Applications — Positive WB detected in | HeLa cells, RAW 264.7 cells, HEK-293 cells, pig brain tissue, HepG2 cells, Jurkat cells, HSC-T6 cells, rabbit brain tissue |
| Tested Applications — Positive IHC detected in | mouse heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Tested Applications — Positive IF/ICC detected in | C2C12 cells |
| Tested Applications — Positive FC (Intra) detected in | A431 cells |
| Recommended Dilutions — Western Blot (WB) | WB : 1:5000-1:50000 |
| Recommended Dilutions — Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Recommended Dilutions — Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| Recommended Dilutions — Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| Positive WB detected in | HeLa cells, RAW 264.7 cells, HEK-293 cells, pig brain tissue, HepG2 cells, Jurkat cells, HSC-T6 cells, rabbit brain tissue |
| Positive IHC detected in | mouse heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | C2C12 cells |
| Positive FC (Intra) detected in | A431 cells |
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| Tested Reactivity | human, mouse, rat, pig, rabbit |
| Cited Reactivity | human, mouse, rat, mosquito |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | CatNo: Ag28383 Product name: Recombinant human a-Actinin protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 543-892 aa of BC015766 Sequence: EIQGLTTAHEQFKATLPDADKERLAILGIHNEVSKIVQTYHVNMAGTNPYTTITPQEINGKWDHVRQLVPRRDQALTEEHARQQHNERLRKQFGAQANVIGPWIQTKMEEIGRISIEMHGTLEDQLSHLRQYEKSIVNYKPKIDQLEGDHQLIQEALIFDNKHTNYTMEHIRVGWEQLLTTIARTINEVENQILTRDAKGISQEQMNEFRASFNHFDRDHSGTLGPEEFKACLISLGYDIGNDPQGEAEFARIMSIVDPNRLGVVTFQAFIDFMSRETADTDTADQVMASFKILAGDKNYITMDELRRELPPDQAEYCIARMAPYTGPDSVPGALDYMSFSTALYGESDL Predict reactive species |
| Full Name | actinin, alpha 1 |
| Calculated Molecular Weight | 103 kDa |
| Observed Molecular Weight | 100-105 kDa |
| GenBank Accession Number | BC015766 |
| Gene Symbol | Alpha Actinin |
| Gene ID (NCBI) | 87 |
| RRID | AB_2882224 |
| Conjugate | Unconjugated |
| Purification Method | Protein A purification |
| UNIPROT ID | P12814 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| FC protocol for Alpha Actinin antibody 66895-1-Ig | Download protocol |
| IF protocol for Alpha Actinin antibody 66895-1-Ig | Download protocol |
| IHC protocol for Alpha Actinin antibody 66895-1-Ig | Download protocol |
| WB protocol for Alpha Actinin antibody 66895-1-Ig | Download protocol |
| rat | IF Sci Adv Electroactive microneedle augmented stem cell therapy in myocardial infarction. Authors - Wentao Zhang View Article |
| mouse,human | WB Cell Mol Biol Lett APOE2 protects against Aβ pathology by improving neuronal mitochondrial function through ERRα signaling Authors - Zhiyuan Ning View Article |
| rat,human | IF Phytomedicine Vitamin K2 (MK7) alleviates arsenic-induced cardiac hypertrophy by suppressing the EGR3/BOK mitochondrial apoptosis pathway. Authors - Ning Liu View Article |
| Citations | 16 |
| Dilutions | WB : 1:5000-1:50000 IHC : 1:500-1:2000 IF/ICC : 1:200-1:800 FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| Isotype | IgG2b |
| Clonality | Monoclonal |
| Clone Number | 1E2E1 |
| Conjugation | Unconjugated |
Alpha Actinins are highly conserved actin-binding proteins that form antiparallel homodimers with the actin-binding domains on each end of the molecule, allowing crosslinking of actin molecules. There are four alpha actinin isoforms identified: two ubiquitously expressed cytoskeletal isoforms (ACTN1 and ACTN4) and two striated muscle-specific sarcomeric isoforms (ACTN2 and ACTN3). ACTN1 is also alternatively spliced to form smooth muscle or brain specific isoforms. This antibody recognize endogenous ACTN1, while it may cross-react with ACTN2 and ACTN4. Protocols Product Specific Protocols FC protocol for Alpha Actinin antibody 66895-1-Ig Download protocol IF protocol for Alpha Actinin antibody 66895-1-Ig Download protocol IHC protocol for Alpha Actinin antibody 66895-1-Ig Download protocol WB protocol for Alpha Actinin antibody 66895-1-Ig Download protocol Standard Protocols Click here to view our Standard Protocols
Publications
- A self-locking conductive cardiac patch for immediate electrical integration with infarcted rat myocardium.
Journal: Bioact Mater | Authors: Mengqi Shan | Species: rat | Application: WB - Dysregulated Rbfox2 produces aberrant splicing of CaV1.2 calcium channel in diabetes-induced cardiac hypertrophy
Journal: Cardiovasc Diabetol | Authors: Pengpeng Li | Species: rat | Application: IF - Electroactive microneedle augmented stem cell therapy in myocardial infarction.
Journal: Sci Adv | Authors: Wentao Zhang | Species: rat | Application: IF - APOE2 protects against Aβ pathology by improving neuronal mitochondrial function through ERRα signaling
Journal: Cell Mol Biol Lett | Authors: Zhiyuan Ning | Species: mouse,human | Application: WB - Macrophage-derived S100A9 promotes diabetic cardiomyopathy by disturbing mitochondrial quality control via STAT3 activation
Journal: Int J Biol Sci | Authors: Shengqi Huo - Vitamin K2 (MK7) alleviates arsenic-induced cardiac hypertrophy by suppressing the EGR3/BOK mitochondrial apoptosis pathway.
Journal: Phytomedicine | Authors: Ning Liu | Species: rat,human | Application: IF - Machine-Learning-Driven Discovery of N-Phenylbenzenesulfonamides as a Novel Chemotype for Lactate Dehydrogenase A Inhibition with Anti-Pancreatic Cancer Activity
Journal: J Med Chem | Authors: Qijie Gong | Species: human | Application: WB - SGK3 deficiency in macrophages suppresses angiotensin II-induced cardiac remodeling via regulating Ndufa13-mediated mitochondrial oxidative stress
Journal: Cell Mol Life Sci | Authors: Jiayu Ren | Species: rat | Application: IF - Pro-Inflammatory Activation Promotes Atherogenic Endothelial Phenotype in Male and Female Human Umbilical Endothelial Vein Cells (HUVECs).
Journal: Int J Mol Sci | Authors: Mario Lorenz | Species: human | Application: WB - Chronic Administration of COVID-19 Drugs Fluvoxamine and Lopinavir Shortens Action Potential Duration by Inhibiting the Human Ether-à-go-go-Related Gene and Cav1.2
Journal: Front Pharmacol | Authors: Zequn Zheng | Species: human | Application: IF - Impaired retinoic acid signaling mediated Rbm20 downregulation induces aberrant splicing of Ca(V)1.2 calcium channel: implications in myocardial hypertrophy.
Journal: J Mol Cell Cardiol | Authors: Xinyu Song | Species: rat | Application: IF - Interplay between autophagy and Sindbis virus in cells derived from key arbovirus vectors, Aedes albopictus and Aedes aegypti mosquitoes
Journal: Cell Signal | Authors: Jialu Qiao | Species: mosquito | Application: WB - Challenges of Assessing Exon 53 Skipping of the Human DMD Transcript with Locked Nucleic Acid-Modified Antisense Oligonucleotides in a Mouse Model for Duchenne Muscular Dystrophy
Journal: Nucleic Acid Ther | Authors: Sarah Engelbeen | Species: mouse | Application: WB - Design, synthesis, and biological evaluation of naphthalene imidazo[1,2-b]pyridazine hybrid derivatives as VEGFR selective inhibitors
Journal: Arch Pharm (Weinheim) | Authors: Shuang Wang | Species: human | Application: WB - Pentose phosphate pathway-derived NADPH facilitates physiological hypertrophy and alleviates ischemia-reperfusion injury in the heart.
Journal: Nat Metab | Authors: Dan Wu - Four new mouse models of Duchenne muscular dystrophy with clinically relevant exon deletions in the human DMD gene.
Journal: Dis Model Mech | Authors: Maaike van Putten
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