CTBP1 Polyclonal antibody 10972-1-AP proteintech
$149.00
In stock
SKU
10972-1-AP
| Catalog Number | 10972-1-AP |
| Synonyms | BARS, CTBP, C-terminal-binding protein 1, EC:1.1.1.- |
| Host | Rabbit |
| Reactivity | human, mouse |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ChIP, ELISA |
| Applications | WB, IHC, IF/ICC, FC (Intra), IP, ELISA |
| Host / Isotype | Rabbit / IgG |
| Tested Applications — Positive WB detected in | HEK-293 cells, mouse skeletal muscle tissue, mouse thymus tissue, mouse brain tissue, HepG2 cells, Jurkat cells, NIH/3T3 cells, SH-SY5Y cells, HeLa cekks |
| Tested Applications — Positive IP detected in | mouse brain tissue |
| Tested Applications — Positive IHC detected in | human prostate cancer tissue, mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Tested Applications — Positive IF/ICC detected in | HepG2 cells |
| Tested Applications — Positive FC (Intra) detected in | HepG2 cells |
| Recommended Dilutions — Western Blot (WB) | WB : 1:1000-1:8000 |
| Recommended Dilutions — Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Recommended Dilutions — Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Recommended Dilutions — Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Recommended Dilutions — Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| Positive WB detected in | HEK-293 cells, mouse skeletal muscle tissue, mouse thymus tissue, mouse brain tissue, HepG2 cells, Jurkat cells, NIH/3T3 cells, SH-SY5Y cells, HeLa cekks |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | human prostate cancer tissue, mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
| Positive FC (Intra) detected in | HepG2 cells |
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CatNo: Ag1425 Product name: Recombinant human CTBP1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-440 aa of BC011655 Sequence: MGSSHLLNKGLPLGVRPPIMNGPLHPRPLVALLDGRDCTVEMPILKDVATVAFCDAQSTQEIHEKVLNEAVGALMYHTITLTREDLEKFKALRIIVRIGSGFDNIDIKSAGDLGIAVCNVPAASVEETADSTLCHILNLYRRATWLHQALREGTRVQSVEQIREVASGAARIRGETLGIIGLGRVGQAVALRAKAFGFNVLFYDPYLSDGVERALGLQRVSTLQDLLFHSDCVTLHCGLNEHNHHLINDFTVKQMRQGAFLVNTARGGLVDEKALAQALKEGRIRGAALDVHESEPFSFSQGPLKDAPNLICTPHAAWYSEQASIEMREEAAREIRRAITGRIPDSLKNCVNKDHLTAATHWASMDPAVVHPELNGAAYRYPPGVVGVAPTGIPAAVEGIVPSAMSLSHGLPPVAHPPHAPSPGQTVKPEADRDHASDQL Predict reactive species |
| Full Name | C-terminal binding protein 1 |
| Calculated Molecular Weight | 48 kDa |
| Observed Molecular Weight | 48 kDa |
| GenBank Accession Number | BC011655 |
| Gene Symbol | CTBP1 |
| Gene ID (NCBI) | 1487 |
| RRID | AB_2086628 |
| Conjugate | Unconjugated |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q13363 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| FC protocol for CTBP1 antibody 10972-1-AP | Download protocol |
| IF protocol for CTBP1 antibody 10972-1-AP | Download protocol |
| IHC protocol for CTBP1 antibody 10972-1-AP | Download protocol |
| IP protocol for CTBP1 antibody 10972-1-AP | Download protocol |
| WB protocol for CTBP1 antibody 10972-1-AP | Download protocol |
| mouse | WB Front Physiol Ling-gui-zhu-gan granules reduces obesity and ameliorates metabolic disorders by inducing white adipose tissue browning in obese mice Authors - Yuxiu Li View Article |
| human | WB Mol Carcinog Role of miR-214 in regulation of β-catenin and the malignant phenotype of melanoma. Authors - Kirthana Prabhakar KD Validated View Article |
| Tongbin (Verified Customer) (02-18-2020) | This antibody worked perfectly. I had a specific band at ~48kD, which is at the predicted size of Ctbp1. Highly recommended for detecting Ctbp1 by western blots! Applications: Western Blot, Primary Antibody Dilution: 1:1000 Cell Tissue Type: Mouse heart |
| Citations | 11 |
| Dilutions | WB : 1:1000-1:8000 IP : 0.5-4.0 ug for IP and 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate for WB IHC : 1:50-1:500 IF/ICC : 1:50-1:500 FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| Isotype | IgG |
| Clonality | Polyclonal |
| Clone Number | - |
| Conjugation | Unconjugated |
CTBP1, also named as C-terminal-binding protein 1, is a 440 amino acid protein, which belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family. CTBP1 is a cellular phosphoprotein that associates with various proteins and functions as a corepressor of transcription. CTBP1 and the related protein CTBP2 are characterized as C-terminal binding protein of adenovirus E1A, and they preferentially associate with the E1A via a 5-amino acid motif, PLDLS, to repress E1A induced oncogenesis and cellular transformation. CTBP1 is expressed from embryo to adult, but CTBP2 is mainly expressed during embryogenesis. Protocols Product Specific Protocols FC protocol for CTBP1 antibody 10972-1-AP Download protocol IF protocol for CTBP1 antibody 10972-1-AP Download protocol IHC protocol for CTBP1 antibody 10972-1-AP Download protocol IP protocol for CTBP1 antibody 10972-1-AP Download protocol WB protocol for CTBP1 antibody 10972-1-AP Download protocol Standard Protocols Click here to view our Standard Protocols
Publications
- CDYL suppresses epileptogenesis in mice through repression of axonal Nav1.6 sodium channel expression.
Journal: Nat Commun | Authors: Yongqing Liu | Species: mouse | Application: WB - ZNF516 suppresses EGFR by targeting the CtBP/LSD1/CoREST complex to chromatin.
Journal: Nat Commun | Authors: Lifang Li KD Validated | Species: human | Application: WB - RBBP8/CtIP suppresses P21 expression by interacting with CtBP and BRCA1 in gastric cancer.
Journal: Oncogene | Authors: Yang Yu | Species: human | Application: WB,IF - Role of hepatoma-derived growth factor in promoting de novo lipogenesis and tumorigenesis in hepatocellular carcinoma.
Journal: Mol Oncol | Authors: Xuejie Min | Species: human | Application: WB - Role of miR-214 in regulation of β-catenin and the malignant phenotype of melanoma.
Journal: Mol Carcinog | Authors: Kirthana Prabhakar KD Validated | Species: human | Application: WB - Ling-gui-zhu-gan granules reduces obesity and ameliorates metabolic disorders by inducing white adipose tissue browning in obese mice
Journal: Front Physiol | Authors: Yuxiu Li | Species: mouse | Application: WB
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