SYK Monoclonal antibody 66721-1-Ig proteintech
$149.00
In stock
SKU
66721-1-Ig
| Catalog Number | 66721-1-Ig |
| Synonyms | 4C4A12, EC:2.7.10.2, IMD82, p72-Syk, spleen tyrosine kinase |
| Host | Mouse |
| Reactivity | human and More (2) |
| Reactivity | human, mouse, pig |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, IHC, IF-P, IP, ELISA |
| Host / Isotype | Mouse / IgG2b |
| Tested Applications — Positive WB detected in | Raji cells, THP-1 cells, Ramos cells, Daudi cells |
| Tested Applications — Positive IP detected in | MDA-MB-231 cells |
| Tested Applications — Positive IHC detected in | human tonsillitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Tested Applications — Positive IF-P detected in | human tonsillitis tissue |
| Recommended Dilutions — Western Blot (WB) | WB : 1:2000-1:10000 |
| Recommended Dilutions — Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Recommended Dilutions — Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Recommended Dilutions — Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| Positive WB detected in | Raji cells, THP-1 cells, Ramos cells, Daudi cells |
| Positive IP detected in | MDA-MB-231 cells |
| Positive IHC detected in | human tonsillitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | human tonsillitis tissue |
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| Tested Reactivity | human |
| Cited Reactivity | human, mouse, pig |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | CatNo: Ag17654 Product name: Recombinant human SYK protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 239-427 aa of BC001645 Sequence: QLVEHYSYKADGLLRVLTVPCQKIGTQGNVNFGGRPQLPGSHPATWSAGGIISRIKSYSFPKPGHRKSSPAQGNRQESTVSFNPYEPELAPWAADKGPQREALPMDTEVYESPYADPEEIRPKEVYLDRKLLTLEDKELGSGNFGTVKKGYYQMKKVVKTVAVKILKNEANDPALKDELLAEANVMQQL Predict reactive species |
| Full Name | spleen tyrosine kinase |
| Calculated Molecular Weight | 635 aa, 72 kDa |
| Observed Molecular Weight | 70-75 kDa |
| GenBank Accession Number | BC001645 |
| Gene Symbol | SYK |
| Gene ID (NCBI) | 6850 |
| ENSEMBL Gene ID | ENSG00000165025 |
| RRID | AB_2882072 |
| Conjugate | Unconjugated |
| Purification Method | Protein A purification |
| UNIPROT ID | P43405 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| IF protocol for SYK antibody 66721-1-Ig | Download protocol |
| IHC protocol for SYK antibody 66721-1-Ig | Download protocol |
| WB protocol for SYK antibody 66721-1-Ig | Download protocol |
| mouse | WB,IF Cell Rep Podocyte SIRPα reduction aggravates lupus nephritis via promoting T cell inflammatory responses Authors - Bin Qian View Article |
| pig,mouse | WB PLoS Pathog Pseudorabies Virus UL4 protein promotes the ASC-dependent inflammasome activation and pyroptosis to exacerbate inflammation Authors - Xiaohua Zhang View Article |
| Citations | 9 |
| Dilutions | WB : 1:2000-1:10000 IP : 0.5-4.0 ug for IP and 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate for WB IHC : 1:500-1:2000 IF-P : 1:50-1:500 |
| Isotype | IgG2b |
| Clonality | Monoclonal |
| Clone Number | 4C4A12 |
| Conjugation | Unconjugated |
SYK(spleen tyrosine kinase) is a key regulator of signal transduction events,apoptosis and orderly cell cycle progression in B-lineage lymphoid cells. Although SYK has not been linked to a human disease, defective expression of the closely related T-cell tyrosine kinase ZAP-70 has been associated with severe combined immunodeficiency(PMID:11494125). This protein has 2 isoforms produced by alternative splicing. SYK also promotes liver fibrosis by activation of hepatic stellate cells and acts as a biomarker for human hepatocellular carcinoma. (PMID: 29537660) Protocols Product Specific Protocols IF protocol for SYK antibody 66721-1-Ig Download protocol IHC protocol for SYK antibody 66721-1-Ig Download protocol WB protocol for SYK antibody 66721-1-Ig Download protocol Standard Protocols Click here to view our Standard Protocols
Publications
- Infiltrating plasma cells maintain glioblastoma stem cells through IgG-Tumor binding
Journal: Cancer Cell | Authors: Jiancheng Gao | Application: WB - Distinct roles of TREM2 in central nervous system cancers and peripheral cancers
Journal: Cancer Cell | Authors: Jian Zhong | Species: mouse | Application: WB - SIGLEC15 amplifies immunosuppressive properties of tumor-associated macrophages in pancreatic cancer.
Journal: Cancer Lett | Authors: Tian-Jiao Li | Species: mouse | Application: WB - Podocyte SIRPα reduction aggravates lupus nephritis via promoting T cell inflammatory responses
Journal: Cell Rep | Authors: Bin Qian | Species: mouse | Application: WB,IF - Troxerutin suppresses the stemness of hepatocellular carcinoma via the Syk/FOXO3 feedback loop.
Journal: Cell Biol Toxicol | Authors: Shan Liu - Pseudorabies Virus UL4 protein promotes the ASC-dependent inflammasome activation and pyroptosis to exacerbate inflammation
Journal: PLoS Pathog | Authors: Xiaohua Zhang | Species: pig,mouse | Application: WB - CD93 promotes acute myeloid leukemia development and is a potential therapeutic target
Journal: Exp Cell Res | Authors: Jie Jia | Species: human | Application: WB - Inhibition of Dectin-1 alleviates inflammation in early diabetic retinopathy by regulating microglia phenotype
Journal: Gene | Authors: Wei Sang | Species: mouse | Application: WB,IF - Identification of high-performing antibodies for tyrosine-protein kinase SYK for use in Western Blot, immunoprecipitation and immunofluorescence
Journal: F1000Res | Authors: Walaa Alshafie | Species: human | Application: WB,IP,IF
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