ATF5 Monoclonal antibody 67066-1-Ig proteintech
$149.00
In stock
SKU
67066-1-Ig
| Catalog Number | 67066-1-Ig |
| Synonyms | ATF5, ATFX, FLJ34666, HMFN0395, Transcription factor ATFx |
| Host | Mouse |
| Reactivity | Human and More (4) |
| Reactivity | human, mouse, rat, chicken |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, IF, ELISA |
| Applications | WB, ELISA |
| Host / Isotype | Mouse / IgG2b |
| Tested Applications — Positive WB detected in | Jurkat cells, HeLa cells, HEK-293 cells, SH-SY5Y cells, A431 cells, HT-29 cells, MCF-7 cells, MDA-MB-231 cells, SK-BR-3 cells, T-47D cells |
| Recommended Dilutions — Western Blot (WB) | WB : 1:2000-1:16000 |
| Positive WB detected in | Jurkat cells, HeLa cells, HEK-293 cells, SH-SY5Y cells, A431 cells, HT-29 cells, MCF-7 cells, MDA-MB-231 cells, SK-BR-3 cells, T-47D cells |
| Western Blot (WB) | WB : 1:2000-1:16000 |
| Tested Reactivity | Human |
| Cited Reactivity | human, mouse, rat, chicken |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | CatNo: Ag7214 Product name: Recombinant human ATF5 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 1-282 aa of BC005174 Sequence: MSLLATLGLELDRALLPASGLGWLVDYGKLPPAPAPLAPYEVLGGALEGGLPVGGEPLAGDGFSDWMTERVDFTALLPLEPPLPPGTLPQPSPTPPDLEAMASLLKKELEQMEDFFLDAPLLPPPSPPPLPPPPLPPAPSLPLSLPSFDLPQPPVLDTLDLLAIYCRNEAGQEEVGMPPLPPPQQPPPPSPPQPSRLAPYPHPATTRGDRKQKKRDQNKSAALRYRQRKRAEGEALEGECQGLEARNRELKERAESVEREIQYVKDLLIEVYKARSQRTRSC Predict reactive species |
| Full Name | activating transcription factor 5 |
| Calculated Molecular Weight | 31 kDa |
| Observed Molecular Weight | 30-35 kDa |
| GenBank Accession Number | BC005174 |
| Gene Symbol | ATF5 |
| Gene ID (NCBI) | 22809 |
| RRID | AB_2882376 |
| Conjugate | Unconjugated |
| Purification Method | Protein A purification |
| UNIPROT ID | Q9Y2D1 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| WB protocol for ATF5 antibody 67066-1-Ig | Download protocol |
| mouse | WB J Muscle Res Cell Motil CD36 regulates myogenic differentiation via chronic integrated stress response - implications for muscle aging. Authors - Xin Ye View Article |
| human,mouse | WB Int J Mol Sci The Mitochondrial PHB2/OMA1/DELE1 Pathway Cooperates with Endoplasmic Reticulum Stress to Facilitate the Response to Chemotherapeutics in Ovarian Cancer. Authors - Meiyu Cheng View Article |
| human | WB FEBS Open Bio FAM136A depletion induces mitochondrial stress and reduces mitochondrial membrane potential and ATP production Authors - Yushi Otsuka View Article |
| rat | WB,IF Stem Cell Res Ther From collapse to comeback: Luteolin rejuvenates nucleus pulposus progenitor cells via SIRT1/ATF5-UPRmt to reverse intervertebral disc degeneration cascade. Authors - Huofeng Wu View Article |
| Christin (Verified Customer) (12-22-2025) | Good antibody for western blot Applications: Western Blot Primary Antibody Dilution: 1:5000 Cell Tissue Type: Human-iPSC-derived immune cells |
| Morgane (Verified Customer) (09-22-2025) | A couple aspecific bands in higher MW but the corresponding one is much brighter. Applications: Western Blot Primary Antibody Dilution: Primary Cell Tissue Type: HCT116 ATF5 Antibody Western Blot validation (Primary dilution) in HCT116 (Cat no:67066-1-Ig) |
| Citations | 10 |
| Dilutions | WB : 1:2000-1:16000 |
| Isotype | IgG2b |
| Clonality | Monoclonal |
| Clone Number | 1E5B6 |
| Conjugation | Unconjugated |
ATF5, also named as Activating transcription factor 5, is a 282 amino acid protein, which belongs to the bZIP family. ATF5 is Widely expressed with higher expression levels in liver. ATF5 is actively transported to the centrosome and accumulated in the pericentriolar material (PCM) during G1 to M phase via a microtubule-dependent mechanism. During late telophase and cytokinesis, it translocates from the centrosome to the midbody (PMID: 26213385) . ATF5 as a transcription factor that either stimulates or represses gene transcription through binding of different DNA regulatory elements such as cAMP response element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), ATF5-specific response element (ARE) (consensus: 5'-C[CT]TCT[CT]CCTT[AT]-3') but also the amino acid response element (AARE), present in many viral and cellular promoters. Critically ATF5 is involved, often in a cell type-dependent manner, in cell survival, proliferation, and differentiation (PubMed:10373550, PubMed:15358120, PubMed:21212266, PubMed:20654631). Protocols Product Specific Protocols WB protocol for ATF5 antibody 67066-1-Ig Download protocol Standard Protocols Click here to view our Standard Protocols
Publications
- Moderate-intensity interval exercise exacerbates cardiac lipotoxicity in high-fat, high-calories diet-fed mice
Journal: Nat Commun | Authors: Jing Geng | Species: mouse | Application: WB - The Mitochondrial PHB2/OMA1/DELE1 Pathway Cooperates with Endoplasmic Reticulum Stress to Facilitate the Response to Chemotherapeutics in Ovarian Cancer.
Journal: Int J Mol Sci | Authors: Meiyu Cheng | Species: human,mouse | Application: WB - Bavachin induces liver injury and cell apoptosis by targeting Wnt/β-catenin/DRP1 signaling pathway mediated mitochondrial dysfunction
Journal: Toxicol Lett | Authors: Ying Yang | Species: human | Application: WB - CD36 regulates myogenic differentiation via chronic integrated stress response - implications for muscle aging.
Journal: J Muscle Res Cell Motil | Authors: Xin Ye | Species: mouse | Application: WB - From collapse to comeback: Luteolin rejuvenates nucleus pulposus progenitor cells via SIRT1/ATF5-UPRmt to reverse intervertebral disc degeneration cascade.
Journal: Stem Cell Res Ther | Authors: Huofeng Wu | Species: rat | Application: WB,IF - FAM136A depletion induces mitochondrial stress and reduces mitochondrial membrane potential and ATP production
Journal: FEBS Open Bio | Authors: Yushi Otsuka | Species: human | Application: WB - Antitumor efficacy and molecular mechanism of lenvatinib combined with vitamin K2 against hepatocellular carcinoma.
Journal: World J Surg Oncol | Authors: Yuli Zhang | Species: human | Application: WB - FAM136A depletion induces mitochondrial stress and reduces mitochondrial membrane potential and ATP production
Journal: FEBS Open Bio | Authors: Yushi Otsuka | Species: human | Application: WB - Knockdown of TMEM160 leads to an increase in reactive oxygen species generation and the induction of the mitochondrial unfolded protein response
Journal: FEBS Open Bio | Authors: Kosei Yamashita | Species: human | Application: WB - CD36 regulates myogenic differentiation via chronic integrated stress response - implications for muscle aging.
Journal: J Muscle Res Cell Motil | Authors: Xin Ye | Species: mouse | Application: WB - Matrix and intermembrane space-specific mitochondrial stress response in Alzheimer's disease.
Journal: NPJ Dement | Authors: Shatakshi Shukla
Product Documents
Reviews
Write Your Own Review