Galectin-1 Monoclonal antibody 60223-1-Ig proteintech
$149.00
In stock
SKU
60223-1-Ig
| Catalog Number | 60223-1-Ig |
| Synonyms | 3G10D2, Beta-galactoside-binding lectin L-14-I, Gal 1, GAL1, Gal-1 |
| Host | Mouse |
| Reactivity | human |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, IHC, IF/ICC, IF-P, ELISA |
| Host / Isotype | Mouse / IgG1 |
| Tested Applications — Positive WB detected in | HCT 116 cells, human placenta tissue, human heart tissue, HepG2 cells, U2OS cells, A549 cells, HeLa cells, L02 cells |
| Tested Applications — Positive IHC detected in | human colon cancer tissue, human pancreas cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Tested Applications — Positive IF-P detected in | human colon cancer tissue |
| Tested Applications — Positive IF/ICC detected in | U2OS cells, Saos-2 cells, human colon cancer tissue |
| Recommended Dilutions — Western Blot (WB) | WB : 1:5000-1:50000 |
| Recommended Dilutions — Immunohistochemistry (IHC) | IHC : 1:2000-1:8000 |
| Recommended Dilutions — Immunofluorescence (IF)-P | IF-P : 1:2000-1:8000 |
| Recommended Dilutions — Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| Positive WB detected in | HCT 116 cells, human placenta tissue, human heart tissue, HepG2 cells, U2OS cells, A549 cells, HeLa cells, L02 cells |
| Positive IHC detected in | human colon cancer tissue, human pancreas cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | human colon cancer tissue |
| Positive IF/ICC detected in | U2OS cells, Saos-2 cells, human colon cancer tissue |
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:2000-1:8000 |
| Immunofluorescence (IF)-P | IF-P : 1:2000-1:8000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| Tested Reactivity | human |
| Cited Reactivity | human |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | CatNo: Ag2432 Product name: Recombinant human Galectin 1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-135 aa of BC020675 Sequence: MACGLVASNLNLKPGECLRVRGEVAPDAKSFVLNLGKDSNNLCLHFNPRFNAHGDANTIVCNSKDGGAWGTEQREAVFPFQPGSVAEVCITFDQANLTVKLPDGYEFKFPNRLNLEAINYMAADGDFKIKCVAFD Predict reactive species |
| Full Name | lectin, galactoside-binding, soluble, 1 |
| Calculated Molecular Weight | 15 kDa |
| Observed Molecular Weight | 13 kDa |
| GenBank Accession Number | BC020675 |
| Gene Symbol | Galectin-1 |
| Gene ID (NCBI) | 3956 |
| RRID | AB_11182608 |
| Conjugate | Unconjugated |
| Purification Method | Protein G purification |
| UNIPROT ID | P09382 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| IF protocol for Galectin-1 antibody 60223-1-Ig | Download protocol |
| IHC protocol for Galectin-1 antibody 60223-1-Ig | Download protocol |
| WB protocol for Galectin-1 antibody 60223-1-Ig | Download protocol |
| human | WB mBio Herpes simplex virus type 1 modifies the protein composition of extracellular vesicles to promote neurite outgrowth and neuroinfection Authors - Guorong Sun View Article |
| Guorong (Verified Customer) (05-23-2022) | It is excellent with ARPE-19 cells but gives no signal with 293T Applications: Western Blot Primary Antibody Dilution: 1:2000 Cell Tissue Type: 293T and ARPE-19 Galectin-1 Antibody Western Blot validation (1:2000 dilution) in 293T and ARPE-19 (Cat no:60223-1-Ig) |
| Sushmita (Verified Customer) (12-16-2019) | This Galactin-1 antibody is working very fine which I applied to probe in western blot in cancer cell line. Applications: Western Blot, Primary Antibody Dilution: 1:4000 Cell Tissue Type: Pancreatic cancer cell line Galectin-1 Antibody Western Blot, validation (1:4000 dilution) in Pancreatic cancer cell line (Cat no:60223-1-Ig) |
| Citations | 10 |
| Dilutions | WB : 1:5000-1:50000 IHC : 1:2000-1:8000 IF-P : 1:2000-1:8000 IF/ICC : 1:400-1:1600 |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Clone Number | 3G10D2 |
| Conjugation | Unconjugated |
Galectins are a family of animal lectins defined by shared characteristic amino-acid sequences and affinity for β-galactose-containing oligosac-charides (PMID: 8063692). Galectin-1 contains one carbohydrate recognition domain (CRD) and occurs as a monomer as well as a non-covalent homodimer (PMID: 16840800). It is differentially expressed by various normal and pathological tissues. Galectin-1 is a multifunctional protein that is involved in cell adhesion, migration, proliferation, apoptosis, inflammation, tumour transformation and growth (PMID:15785741). Protocols Product Specific Protocols IF protocol for Galectin-1 antibody 60223-1-Ig Download protocol IHC protocol for Galectin-1 antibody 60223-1-Ig Download protocol WB protocol for Galectin-1 antibody 60223-1-Ig Download protocol Standard Protocols Click here to view our Standard Protocols
Publications
- Periplocin suppresses the growth of colorectal cancer cells by triggering LGALS3 (galectin 3)-mediated lysophagy
Journal: Autophagy | Authors: Kui Wang | Species: human - Impact of galectin-1's redox state on its lectin activity and monomer-dimer equilibrium. Focusing on oxidized Gal-1
Journal: Int J Biol Macromol | Authors: Tatiana Staroňová | Species: human | Application: WB - Galectin‑1 binds GRP78 to promote the proliferation and metastasis of gastric cancer
Journal: Int J Oncol | Authors: Qi Zhang | Species: human | Application: IF - Yang-Xue-An-Tai Decoction Treats URSA by regulating the differentiation of proliferating decidual stromal cells through multiple targets.
Journal: J Ethnopharmacol | Authors: Guanshan Li - Galectin-1 exacerbates hepatic steatosis by impairing autophagy via interaction with FIP200.
Journal: Int Immunopharmacol | Authors: Lujuan Zheng - Herpes simplex virus type 1 modifies the protein composition of extracellular vesicles to promote neurite outgrowth and neuroinfection
Journal: mBio | Authors: Guorong Sun | Species: human | Application: WB
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