CoraLite®594-conjugated Caspase 8/P43/P18 Monoclonal antibody CL594-66093 proteintech
$479.00
In stock
SKU
CL594-66093
| Catalog Number | CL594-66093 |
| Synonyms | CASP8, Caspase 8, 2B9H8, Apoptotic cysteine protease, Apoptotic protease Mch-5 |
| Host | Mouse |
| Reactivity | human |
| Form | Liquid |
| Formulation | PBS, Proclin300, BSA, Glycerol |
| Applications | IF/ICC |
| Host / Isotype | Mouse / IgG2b |
| Tested Applications — Positive IF/ICC detected in | HeLa cells |
| Recommended Dilutions — Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Positive IF/ICC detected in | HeLa cells |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Tested Reactivity | human |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | CatNo: Ag20524 Product name: Recombinant human Caspase 8 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 1-270 aa of BC028223 Sequence: MDFSRNLYDIGEQLDSEDLASLKFLSLDYIPQRKQEPIKDALMLFQRLQEKRMLEESNLSFLKELLFRINRLDLLITYLNTRKEEMERELQTPGRAQISAYRVMLYQISEEVSRSELRSFKFLLQEEISKCKLDDDMNLLDIFIEMEKRVILGEGKLDILKRVCAQINKSLLKIINDYEEFSKERSSSLEGSPDEFSNGEELCGVMTISDSPREQDSESQTLDKVYQMKSKPRGYCLIINNHNFAKAREKVPKLHSIRDRNGTHLDAGTV Predict reactive species |
| Full Name | caspase 8, apoptosis-related cysteine peptidase |
| Calculated Molecular Weight | 538 aa, 62 kDa |
| Observed Molecular Weight | 53-57 kDa, 32-45 kDa, 18 kDa |
| GenBank Accession Number | BC028223 |
| Gene Symbol | Caspase 8 |
| Gene ID (NCBI) | 841 |
| RRID | AB_2883508 |
| Conjugate | CoraLite®594 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 588 nm / 604 nm |
| Excitation Laser | Yellow-Green Laser (561 nm) |
| Purification Method | Protein A purification |
| UNIPROT ID | Q14790 |
| Storage Buffer | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. |
| IF protocol for CL594 Caspase 8/P43/P18 antibody CL594-66093 | Download protocol |
| Citations | - |
| Dilutions | IF/ICC : 1:50-1:500 |
| Isotype | IgG2b |
| Clonality | Monoclonal |
| Clone Number | 2B9H8 |
| Conjugation | CoraLite®594 |
Caspase 8, also named as MCH5, MACH, FLICE, and CAP4, belongs to the peptidase C14A family. It may participate in the GZMB apoptotic pathways and functions as an upstream regulator in a-bisabolol-induced apoptosis. Caspase 8 catalyzes an essential intermediate step in the ubiquitination and proteasome-mediated degradation of IRF3 (PMID:21816816). It may control diabetic embryopathy-associated apoptosis via regulation of the Bid-stimulated mitochondrion/caspase-9 pathway (PMID:19194987). Caspase 8 is expressed as nine isoforms by alternative splicing with the molecular mass from 26 kDa to 62 kDa. This antibody can recognize pro- and cleaved-caspase 8. Protocols Product Specific Protocols IF protocol for CL594 Caspase 8/P43/P18 antibody CL594-66093 Download protocol Standard Protocols Click here to view our Standard Protocols
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