Cathepsin L Polyclonal antibody 27952-1-AP proteintech
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SKU
27952-1-AP
| Catalog Number | 27952-1-AP |
| Synonyms | Cathepsin L, cathepsin L1, CATL, CTSL, CTSL1, FLJ31037, Major excreted protein, MEP |
| Host | Rabbit |
| Reactivity | Human, mouse and More (2) |
| Reactivity | human, mouse, rat |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, IHC, IF, IP, ELISA |
| Applications | WB, IHC, ELISA |
| Host / Isotype | Rabbit / IgG |
| Tested Applications — Positive WB detected in | A549 cells |
| Tested Applications — Positive IHC detected in | human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Recommended Dilutions — Western Blot (WB) | WB : 1:500-1:1000 |
| Recommended Dilutions — Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Positive WB detected in | A549 cells |
| Positive IHC detected in | human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Tested Reactivity | Human, mouse |
| Cited Reactivity | human, mouse, rat |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CatNo: Ag27813 Product name: Recombinant human Cathepsin L protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 114-288 aa of BC012612 Sequence: APRSVDWREKGYVTPVKNQGQCGSCWAFSATGALEGQMFRKTGRLISLSEQNLVDCSGPQGNEGCNGGLMDYAFQYVQDNGGLDSEESYPYEATEESCKYNPKYSVANDTGFVDIPKQEKALMKAVATVGPISVAIDAGHESFLFYKEGIYFEPDCSSEDMDHGVLVVGYGFEST Predict reactive species |
| Full Name | cathepsin L1 |
| Calculated Molecular Weight | 38 kDa |
| Observed Molecular Weight | 36-39 kDa, 29 kDa |
| GenBank Accession Number | BC012612 |
| Gene Symbol | Cathepsin L |
| Gene ID (NCBI) | 1514 |
| RRID | AB_2881021 |
| Conjugate | Unconjugated |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P07711 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| IHC protocol for Cathepsin L antibody 27952-1-AP | Download protocol |
| WB protocol for Cathepsin L antibody 27952-1-AP | Download protocol |
| mouse | IF J Transl Med TRIM7-mediated autophagy-lysosomal signaling is essential for preimplantation development in mice. Authors - Sarmir Khan View Article |
| human | WB,IHC,IF World J Gastroenterol Macrophage-derived cathepsin L promotes epithelial-mesenchymal transition and M2 polarization in gastric cancer Authors - Lu-Xi Xiao KD Validated View Article |
| Citations | 22 |
| Dilutions | WB : 1:500-1:1000 IHC : 1:50-1:500 |
| Isotype | IgG |
| Clonality | Polyclonal |
| Clone Number | - |
| Conjugation | Unconjugated |
CTSL1(Cathepsin L1) is also named as CTSL and MEP, belongs to the peptidase C1 family. It is a lysosomal proteinase whose expression is also up-regulated in the skeletal muscle during starvation(PMID:20088826). It plays an intracellular role in normal intestinal epithelial polarization and initiation of neoplasia(PMID:17622569). CTSL1 also improves cardiac function and inhibits cardiac hypertrophy, inflammation, and fibrosis through blocking AKT/GSK3B signaling(PMID:19096818). The full length protein is 38 kDa with a signal peptide, two propeptide and a glycosylation site. It has been detected the 36 kDa, 39 kDa, 29 kDa and 21 kDa in rat gastrocnemius muscle. These forms of cathepsin L could either be attributed to differences in glycosylation or to partial processing of the proenzyme. (PMID:11696001) Protocols Product Specific Protocols IHC protocol for Cathepsin L antibody 27952-1-AP Download protocol WB protocol for Cathepsin L antibody 27952-1-AP Download protocol Standard Protocols Click here to view our Standard Protocols
Publications
- Nodosin Exerts an Anti-Colorectal Cancer Effect by Inhibiting Proliferation and Triggering Complex Cell Death in Vitro and in Vivo
Journal: Front Pharmacol | Authors: Huixia Fan | Species: mouse | Application: IHC - USP20 competitively binds to STUB1 to enhance CTSL expression and promote epithelial-mesenchymal transition in head and neck squamous cell carcinoma.
Journal: Clin Transl Med | Authors: Lunhua Guo KD Validated | Species: human | Application: WB,IP - Microbiome Composition Regulates Cathepsin Expression in Vaginal Fluid Across BV Diagnoses and Community State Types.
Journal: bioRxiv | Authors: Carleigh Coffin Sokolik | Species: human | Application: WB - Tumor cell-released autophagosome (TRAP) programs inflammatory CAFs to drive the immune-excluded TIME through C3a.
Journal: Cell Mol Immunol | Authors: Xuru Wang - Macrophage-derived cathepsin L promotes epithelial-mesenchymal transition and M2 polarization in gastric cancer
Journal: World J Gastroenterol | Authors: Lu-Xi Xiao KD Validated | Species: human | Application: WB,IHC,IF - TRIM7-mediated autophagy-lysosomal signaling is essential for preimplantation development in mice.
Journal: J Transl Med | Authors: Sarmir Khan | Species: mouse | Application: IF - Salvianolic acid B suppresses hepatic fibrosis by inhibiting ceramide glucosyltransferase in hepatic stellate cells
Journal: Acta Pharmacol Sin | Authors: Zi-Bo Li | Species: human | Application: WB - TRIM7-mediated autophagy-lysosomal signaling is essential for preimplantation development in mice.
Journal: J Transl Med | Authors: Sarmir Khan | Species: mouse | Application: IF - USP20 competitively binds to STUB1 to enhance CTSL expression and promote epithelial-mesenchymal transition in head and neck squamous cell carcinoma.
Journal: Clin Transl Med | Authors: Lunhua Guo | Species: human | Application: WB,IP - Macrophage-derived cathepsin L promotes epithelial-mesenchymal transition and M2 polarization in gastric cancer
Journal: World J Gastroenterol | Authors: Lu-Xi Xiao | Species: human | Application: WB,IHC,IF - Semax peptide targets the μ opioid receptor gene Oprm1 to promote deubiquitination and functional recovery after spinal cord injury in female mice.
Journal: Br J Pharmacol | Authors: Rongjie Liu | Species: mouse,rat,human | Application: WB,IF - Design, Synthesis, and Biological Evaluation of 2-Arylaminopyrimidine Derivatives as Dual Cathepsin L and JAK Inhibitors for the Treatment of Acute Lung Injury
Journal: J Med Chem | Authors: Chunwei Shen | Species: mouse | Application: WB - Intranasal drop of multiplex RNA therapy for SARS-CoV-2 infections: baicalein-enhanced FEN1 nuclease cleaving as a virus buster.
Journal: Emerg Microbes Infect | Authors: Jiyanuo Cao - Design, synthesis and biological evaluation of novel pyrrolo[2,3-d]pyrimidine derivatives as cathepsin L inhibitor for the treatment of acute lung injury.
Journal: Eur J Med Chem | Authors: Zhengtong Mao | Species: human, mouse | Application: WB - Design and optimization of N-(3-((4-(1H-indol-3-yl)pyrimidin-2-yl)amino)phenyl)amide derivatives as potent anti-inflammatory agents against LPS-induced acute lung injury.
Journal: Eur J Med Chem | Authors: Zhe Wang - Alumina nanoparticles induce learning and memory impairment in a particle size-dependent and time-dependent manner
Journal: Ecotoxicol Environ Saf | Authors: Ying Zhang | Species: mouse | Application: WB - Nodosin Exerts an Anti-Colorectal Cancer Effect by Inhibiting Proliferation and Triggering Complex Cell Death in Vitro and in Vivo
Journal: Front Pharmacol | Authors: Huixia Fan | Species: mouse | Application: IHC - Mitochondria-related chemoradiotherapy resistance genes-based machine learning model associated with immune cell infiltration on the prognosis of esophageal cancer and its value in pan-cancer
Journal: Transl Oncol | Authors: Ziyu Liu | Species: human | Application: IHC - CTSL Promotes Autophagy in Laryngeal Cancer Through the IL6-JAK-STAT3 Signalling Pathway
Journal: J Cell Mol Med | Authors: Xueying Wang | Species: human,mouse | Application: WB,IHC,IF - Magnolol inhibits porcine epidemic diarrhea virus infection by suppressing cathepsin L expression in vitro and in vivo.
Journal: J Virol | Authors: Yi-fan Liang - Integration of Single-cell and Bulk Transcriptome Analyses Unravels a Macrophage-based Gene Signature for Prognostication and Treatment in Triple-negative Breast Cancer.
Journal: Int J Med Sci | Authors: Yuan Huang | Species: human | Application: WB,IF - Microbiome Composition Regulates Cathepsin Expression in Vaginal Fluid Across BV Diagnoses and Community State Types.
Journal: bioRxiv | Authors: Carleigh Coffin Sokolik | Species: human | Application: WB - Neoliensinine promotes lysosomal cell death in T cell malignancies via a novel STING-TUBA1B pathway.
Journal: Genes Dis | Authors: Po Hu
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