Cytochrome P450 Reductase Polyclonal antibody 29814-1-AP proteintech
$149.00
In stock
SKU
29814-1-AP
| Catalog Number | 29814-1-AP |
| Synonyms | CPR, CYPOR, P450R, POR |
| Host | Rabbit |
| Reactivity | human, mouse, rat |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, IHC, ELISA |
| Host / Isotype | Rabbit / IgG |
| Tested Applications — Positive WB detected in | human placenta tissue, HepG2 cells, mouse liver tissue, rat liver tissue |
| Tested Applications — Positive IHC detected in | mouse kidney tissue, rat kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Recommended Dilutions — Western Blot (WB) | WB : 1:1000-1:6000 |
| Recommended Dilutions — Immunohistochemistry (IHC) | IHC : 1:1500-1:6000 |
| Positive WB detected in | human placenta tissue, HepG2 cells, mouse liver tissue, rat liver tissue |
| Positive IHC detected in | mouse kidney tissue, rat kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunohistochemistry (IHC) | IHC : 1:1500-1:6000 |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CatNo: Ag30899 Product name: Recombinant human POR protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 43-216 aa of NM_000941 Sequence: LFRKKKEEVPEFTKIQTLTSSVRESSFVEKMKKTGRNIIVFYGSQTGTAEEFANRLSKDAHRYGMRGMSADPEEYDLADLSSLPEIDNALVVFCMATYGEGDPTDNAQDFYDWLQETDVDLSGVKFAVFGLGNKTYEHFNAMGKYVDKRLEQLGAQRIFELGLGDDDGNLEEDF Predict reactive species |
| Full Name | P450 (cytochrome) oxidoreductase |
| Calculated Molecular Weight | 77 kDa |
| Observed Molecular Weight | 77 kDa |
| GenBank Accession Number | NM_000941 |
| Gene Symbol | Cytochrome P450 Reductase |
| Gene ID (NCBI) | 5447 |
| RRID | AB_3086171 |
| Conjugate | Unconjugated |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P16435 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| IHC protocol for Cytochrome P450 Reductase antibody 29814-1-AP | Download protocol |
| WB protocol for Cytochrome P450 Reductase antibody 29814-1-AP | Download protocol |
| human | WB iScience AMPD3 promotes doxorubicin-induced cardiomyopathy through HSP90α-mediated ferroptosis Authors - Liting Cheng View Article |
| Citations | 1 |
| Dilutions | WB : 1:1000-1:6000 IHC : 1:1500-1:6000 |
| Isotype | IgG |
| Clonality | Polyclonal |
| Clone Number | - |
| Conjugation | Unconjugated |
Cytochrome P450 Reductase (CYPOR) consists of the flavin mononucleotide (FMN) domain and the flavin adenine dinucleotide (FAD) domain, which bind the corresponding cofactors and transfer electrons from NADPH via FAD and FMN (PMID: 33498551). CYPOR is an essential component of the microsomal P450 mixed-function oxidase system and is required for electron transfer from NADPH to cytochromes P450 in microsomes. The calculated molecular weight of CYPOR is 77 kDa. Protocols Product Specific Protocols IHC protocol for Cytochrome P450 Reductase antibody 29814-1-AP Download protocol WB protocol for Cytochrome P450 Reductase antibody 29814-1-AP Download protocol Standard Protocols Click here to view our Standard Protocols
Publications
- AMPD3 promotes doxorubicin-induced cardiomyopathy through HSP90α-mediated ferroptosis
Journal: iScience | Authors: Liting Cheng | Species: human | Application: WB
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