IRF4 Monoclonal antibody 66451-1-Ig proteintech
$149.00
In stock
SKU
66451-1-Ig
| Catalog Number | 66451-1-Ig |
| Synonyms | MUM1/IRF4, 1A12D5, interferon regulatory factor 4, IRF 4, IRF-4 |
| Host | Mouse |
| Reactivity | human, rat, pig and More (1) |
| Reactivity | human, rat, pig, mouse |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, IHC, IF, CoIP, ELISA |
| Applications | WB, IHC, ELISA |
| Host / Isotype | Mouse / IgG1 |
| Tested Applications — Positive WB detected in | U266 cells, human spleen tissue, pig spleen tissue, rat spleen tissue, HuT 78 cells, MJ cells |
| Tested Applications — Positive IHC detected in | human tonsillitis Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Recommended Dilutions — Western Blot (WB) | WB : 1:2000-1:10000 |
| Recommended Dilutions — Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| Positive WB detected in | U266 cells, human spleen tissue, pig spleen tissue, rat spleen tissue, HuT 78 cells, MJ cells |
| Positive IHC detected in | human tonsillitis Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| Tested Reactivity | human, rat, pig |
| Cited Reactivity | human, mouse, rat, pig |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | CatNo: Ag25203 Product name: Recombinant human IRF4 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 1-451 aa of BC015752 Sequence: MNLEGGGRGGEFGMSAVSCGNGKLRQWLIDQIDSGKYPGLVWENEEKSIFRIPWKHAGKQDYNREEDAALFKAWALFKGKFREGIDKPDPPTWKTRLRCALNKSNDFEELVERSQLDISDPYKVYRIVPEGAKKGAKQLTLEDPQMSMSHPYTMTTPYPSLPAQQVHNYMMPPLDRSWRDYVPDQPHPEIPYQCPMTFGPRGHHWQGPACENGCQVTGTFYACAPPESQAPGVPTEPSIRSAEALAFSDCRLHICLYYREILVKELTTSSPEGCRISHGHTYDASNLDQVLFPYPEDNGQRKNIEKLLSHLERGVVLWMAPDGLYAKRLCQSRIYWDGPLALCNDRPNKLERDQTCKLFDTQQFLSELQAFAHHGRSLPRFQVTLCFGEEFPDPQRQRKLITAHVEPLLARQLYYFAQQNSGHFLRGYDLPEHISNPEDYHRSIRHSSIQE Predict reactive species |
| Full Name | IRF 4 |
| Calculated Molecular Weight | 52 kDa |
| Observed Molecular Weight | 48-52 kDa |
| GenBank Accession Number | BC015752 |
| Gene Symbol | IRF4 |
| Gene ID (NCBI) | 3662 |
| ENSEMBL Gene ID | ENSG00000137265 |
| RRID | AB_2881820 |
| Conjugate | Unconjugated |
| Purification Method | Protein G purification |
| UNIPROT ID | Q15306 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| IHC protocol for IRF4 antibody 66451-1-Ig | Download protocol |
| WB protocol for IRF4 antibody 66451-1-Ig | Download protocol |
| mouse,human | WB,IF Bioeng Transl Med Macrophage-targeted delivery of siRNA to silence Mecp2 gene expression attenuates pulmonary fibrosis. Authors - Yong Mou View Article |
| human | WB Sci Rep Endogenous protein tagging coupled with a CRISPR screening approach identifies UBE3C as a potential MYC oncogene regulator. Authors - Marcel Seibert View Article |
| mouse | WB Br J Cancer Impact of gut microbiome on radiotherapy and immunotherapy efficacy in microsatellite-stable colorectal cancer: role of propionic acid and B. fragilis Authors - Lu Yu View Article |
| rat | IHC Free Radic Biol Med Urban particulate matter induces NLRP3/NLRP6 inflammasomes, autophagy, senescence and fibrotic responses by increasing CSF2 signaling in kidney cells. Authors - Ruei-Yu Su View Article |
| Citations | 10 |
| Dilutions | WB : 1:2000-1:10000 IHC : 1:200-1:800 |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Clone Number | 1A12D5 |
| Conjugation | Unconjugated |
IF regulatory factor 4 (IRF4) is a member of the IRF family of transcription factors, expressed in most cell types of the immune system. IRF4 is a transcription factor essential for the development of T helper-2 (Th2) cells, IL17-producing Th17 cells, and IL9-producing Th9 cells, as well as dendritic cell (DC). It binds to and activates the IF-stimulated response element (ISRE) of the MHC class I promoter, and may have a role in ISRE-targeted signal transduction mechanisms specific to lymphoid cells. Protocols Product Specific Protocols IHC protocol for IRF4 antibody 66451-1-Ig Download protocol WB protocol for IRF4 antibody 66451-1-Ig Download protocol Standard Protocols Click here to view our Standard Protocols
Publications
- Macrophage-targeted delivery of siRNA to silence Mecp2 gene expression attenuates pulmonary fibrosis.
Journal: Bioeng Transl Med | Authors: Yong Mou | Species: mouse,human | Application: WB,IF - RNF2 promotes the progression of colon cancer by regulating ubiquitination and degradation of IRF4
Journal: Biochim Biophys Acta Mol Cell Res | Authors: Jiwei Wang | Species: human | Application: WB,CoIP - TBK1 is involved in M-CSF-induced macrophage polarization through mediating the IRF5/IRF4 axis
Journal: FEBS J | Authors: Yuanyuan Li | Species: mouse | Application: WB,IF - Urban particulate matter induces NLRP3/NLRP6 inflammasomes, autophagy, senescence and fibrotic responses by increasing CSF2 signaling in kidney cells.
Journal: Free Radic Biol Med | Authors: Ruei-Yu Su | Species: rat | Application: IHC - Endogenous protein tagging coupled with a CRISPR screening approach identifies UBE3C as a potential MYC oncogene regulator.
Journal: Sci Rep | Authors: Marcel Seibert | Species: human | Application: WB - Impact of gut microbiome on radiotherapy and immunotherapy efficacy in microsatellite-stable colorectal cancer: role of propionic acid and B. fragilis
Journal: Br J Cancer | Authors: Lu Yu | Species: mouse | Application: WB - Irf4 participates in benzene-induced hematopoietic senescence through mitochondrial ROS-dependent BCAA catabolism
Journal: Toxicology | Authors: Ziyan Liu | Species: mouse | Application: WB,IHC - Endogenous protein tagging coupled with a CRISPR screening approach identifies UBE3C as a potential MYC oncogene regulator.
Journal: Sci Rep | Authors: Marcel Seibert | Species: human | Application: WB - RNF2 promotes the progression of colon cancer by regulating ubiquitination and degradation of IRF4
Journal: Biochim Biophys Acta Mol Cell Res | Authors: Jiwei Wang | Species: human | Application: WB,CoIP - TBK1 is involved in M-CSF-induced macrophage polarization through mediating the IRF5/IRF4 axis
Journal: FEBS J | Authors: Yuanyuan Li | Species: mouse | Application: WB,IF
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