Cytokeratin 8 Monoclonal antibody 68627-1-Ig proteintech
$149.00
In stock
SKU
68627-1-Ig
| Catalog Number | 68627-1-Ig |
| Synonyms | KRT8, 1F2H5, CARD2, CK 8, CK8 |
| Host | Mouse |
| Reactivity | human, mouse, rat |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, IHC, IF, ELISA |
| Applications | WB, IHC, ELISA |
| Host / Isotype | Mouse / IgG1 |
| Tested Applications — Positive WB detected in | MCF-7 cells, A549 cells, rat colon tissue, mouse colon tissue, T-47D cells, SK-BR-3 cells, SW480 cells, HT-29 cells |
| Tested Applications — Positive IHC detected in | human colon tissue, human hepatocellular carcinoma, human tonsillitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Recommended Dilutions — Western Blot (WB) | WB : 1:5000-1:50000 |
| Recommended Dilutions — Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| Positive WB detected in | MCF-7 cells, A549 cells, rat colon tissue, mouse colon tissue, T-47D cells, SK-BR-3 cells, SW480 cells, HT-29 cells |
| Positive IHC detected in | human colon tissue, human hepatocellular carcinoma, human tonsillitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | CatNo: Ag0488 Product name: Recombinant human KRT8 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-483 aa of BC000654 Sequence: MSIRVTQKSYKVSTSGPRAFSSRSYTSGPGSRISSSSFSRVGSSNFRGGLGGGYGGASGMGGITAVTVNQSLLSPLVLEVDPNIQAVRTQEKEQIKTLNNKFASFIDKVRFLEQQNKMLETKWSLLQQQKTARSNMDNMFESYINNLRRQLETLGQEKLKLEAELGNMQGLVEDFKNKYEDEINKRTEMENEFVLIKKDVDEAYMNKVELESRLEGLTDEINFLRQLYEEEIRELQSQISDTSVVLSMDNSRSLDMDSIIAEVKAQYEDIANRSRAEAESMYQIKYEELQSLAGKHGDDLRRTKTEISEMNRNISRLQAEIEGLKGQRASLEAAIADAEQRGELAIKDANAKLSELEAALQRAKQDMARQLREYQELMNVKLALDIEIATYRKLLEGEESRLESGMQNMSIHTKTTSGYAGGLSSAYGGLTSPGLSYSLGSSFGSGAGSSSFSRTSSSRAVVVKKIETRDGKLVSESSDVLPK Predict reactive species |
| Full Name | keratin 8 |
| Calculated Molecular Weight | 54 kDa |
| Observed Molecular Weight | 50-52 kDa |
| GenBank Accession Number | BC000654 |
| Gene Symbol | Cytokeratin 8 |
| Gene ID (NCBI) | 3856 |
| RRID | AB_3085319 |
| Conjugate | Unconjugated |
| Purification Method | Protein G purification |
| UNIPROT ID | P05787 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| IHC protocol for Cytokeratin 8 antibody 68627-1-Ig | Download protocol |
| WB protocol for Cytokeratin 8 antibody 68627-1-Ig | Download protocol |
| mouse,human | IF Nat Biomed Eng Mammary organoid-based depot for post-surgical chemotherapy and gland regeneration. Authors - Shenqiang Wang View Article |
| Citations | 1 |
| Dilutions | WB : 1:5000-1:50000 IHC : 1:250-1:1000 |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Clone Number | 1F2H5 |
| Conjugation | Unconjugated |
Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. KRT8 is often paired with keratin 18 in vivo. Protocols Product Specific Protocols IHC protocol for Cytokeratin 8 antibody 68627-1-Ig Download protocol WB protocol for Cytokeratin 8 antibody 68627-1-Ig Download protocol Standard Protocols Click here to view our Standard Protocols
Publications
- Mammary organoid-based depot for post-surgical chemotherapy and gland regeneration.
Journal: Nat Biomed Eng | Authors: Shenqiang Wang | Species: mouse,human | Application: IF
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