DCPS Polyclonal antibody 28465-1-AP proteintech
$149.00
In stock
SKU
28465-1-AP
| Catalog Number | 28465-1-AP |
| Synonyms | DCS 1, DCS1, DCS-1, decapping enzyme, scavenger, Decapping scavenger enzyme |
| Host | Rabbit |
| Reactivity | human, mouse |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, IHC, IF/ICC, ELISA |
| Host / Isotype | Rabbit / IgG |
| Tested Applications — Positive WB detected in | A549 cells, HeLa cells, HepG2 cells, mouse testis tissue |
| Tested Applications — Positive IHC detected in | mouse testis tissue, human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Tested Applications — Positive IF/ICC detected in | HeLa cells |
| Recommended Dilutions — Western Blot (WB) | WB : 1:500-1:1000 |
| Recommended Dilutions — Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Recommended Dilutions — Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| Positive WB detected in | A549 cells, HeLa cells, HepG2 cells, mouse testis tissue |
| Positive IHC detected in | mouse testis tissue, human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| Tested Reactivity | human, mouse |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CatNo: Ag29351 Product name: Recombinant human DCPS protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 7-160 aa of BC014532 Sequence: QLGKRKRELDVEEAHAASTEEKEAGVGNGTCAPVRLPFSGFRLQKVLRESARDKIIFLHGKVNEASEDGDGEDAVVILEKTPFQVEQVAQLLTGSPELQLQFSNDIYSTYHLFPPRQLNDVKTTVVYPATEKHLQKYLRQDLRLIRETGDDYRN Predict reactive species |
| Full Name | decapping enzyme, scavenger |
| Calculated Molecular Weight | 337 aa, 39 kDa |
| Observed Molecular Weight | 35-40 kDa |
| GenBank Accession Number | BC014532 |
| Gene Symbol | DCPS |
| Gene ID (NCBI) | 28960 |
| RRID | AB_3086054 |
| Conjugate | Unconjugated |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q96C86 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| IF protocol for DCPS antibody 28465-1-AP | Download protocol |
| IHC protocol for DCPS antibody 28465-1-AP | Download protocol |
| WB protocol for DCPS antibody 28465-1-AP | Download protocol |
| Citations | - |
| Dilutions | WB : 1:500-1:1000 IHC : 1:50-1:500 IF/ICC : 1:200-1:800 |
| Isotype | IgG |
| Clonality | Polyclonal |
| Clone Number | - |
| Conjugation | Unconjugated |
DcpS, which is a member of the histidine triad (HIT) superfamily of pyrophosphatases, is the first HIT protein with a defined biological function and identifies the HIT motif as a new mRNA decapping domain. DcpS can encode a 38-40 kDa protein, and has the potential to form a homodimer of 80 kDa(PMID: 12198172). Protocols Product Specific Protocols IF protocol for DCPS antibody 28465-1-AP Download protocol IHC protocol for DCPS antibody 28465-1-AP Download protocol WB protocol for DCPS antibody 28465-1-AP Download protocol Standard Protocols Click here to view our Standard Protocols