ATP5C1 Monoclonal antibody 60284-1-Ig proteintech
$149.00
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SKU
60284-1-Ig
| Catalog Number | 60284-1-Ig |
| Synonyms | 1B5A2, ATP synthase F1 subunit gamma, ATP5C, ATP5CL1, ATP5F1C |
| Host | Mouse |
| Reactivity | human, pig, rabbit and More (1) |
| Reactivity | human, pig, rabbit, zebrafish |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, IHC, IF/ICC, FC (Intra), ELISA |
| Host / Isotype | Mouse / IgG2a |
| Tested Applications — Positive WB detected in | human heart tissue, fetal human brain tissue, HeLa cells, Caki-1 cells, human skeletal muscle tissue, TF-1 cells, Rabbit heart tissue, Pig heart tissue |
| Tested Applications — Positive IHC detected in | human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Tested Applications — Positive IF/ICC detected in | HepG2 cells |
| Tested Applications — Positive FC (Intra) detected in | HeLa cells |
| Recommended Dilutions — Western Blot (WB) | WB : 1:500-1:2000 |
| Recommended Dilutions — Immunohistochemistry (IHC) | IHC : 1:100-1:400 |
| Recommended Dilutions — Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Recommended Dilutions — Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| Positive WB detected in | human heart tissue, fetal human brain tissue, HeLa cells, Caki-1 cells, human skeletal muscle tissue, TF-1 cells, Rabbit heart tissue, Pig heart tissue |
| Positive IHC detected in | human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
| Positive FC (Intra) detected in | HeLa cells |
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:100-1:400 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| Tested Reactivity | human, pig, rabbit |
| Cited Reactivity | human, zebrafish |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | CatNo: Ag1343 Product name: Recombinant human ATP5C1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-298 aa of BC000931 Sequence: MFSRAGVAGLSAWTLQPQWIQVRNMATLKDITRRLKSIKNIQKITKSMKMVAAAKYARAERELKPARIYGLGSLALYEKADIKGPEDKKKHLLIGVSSDRGLCGAIHSSIAKQMKSEVATLTAAGKEVMLVGIGDKIRGILYRTHSDQFLVAFKEVGRKPPTFGDASVIALELLNSGYEFDEGSIIFNKFRSVISYKTEEKPIFSLNTVASADSMSIYDDIDADVLQNYQEYNLANIIYYSLKESTTSEQSARMTAMDNASKNASEMIDKLTLTFNRTRQAVITKELIEIISGAAALD Predict reactive species |
| Full Name | ATP synthase, H+ transporting, mitochondrial F1 complex, gamma polypeptide 1 |
| Calculated Molecular Weight | 33 kDa |
| Observed Molecular Weight | 33 kDa |
| GenBank Accession Number | BC000931 |
| Gene Symbol | ATP5C1 |
| Gene ID (NCBI) | 509 |
| RRID | AB_2881402 |
| Conjugate | Unconjugated |
| Purification Method | Protein A purification |
| UNIPROT ID | P36542 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| FC protocol for ATP5C1 antibody 60284-1-Ig | Download protocol |
| IF protocol for ATP5C1 antibody 60284-1-Ig | Download protocol |
| IHC protocol for ATP5C1 antibody 60284-1-Ig | Download protocol |
| WB protocol for ATP5C1 antibody 60284-1-Ig | Download protocol |
| human | WB Nat Cell Biol Small heat shock proteins operate as molecular chaperones in the mitochondrial intermembrane space Authors - Elias Adriaenssens View Article |
| zebrafish | WB Nucleic Acids Res Deletion of Gtpbp3 in zebrafish revealed the hypertrophic cardiomyopathy manifested by aberrant mitochondrial tRNA metabolism. Authors - Danni Chen View Article |
| Citations | 5 |
| Dilutions | WB : 1:500-1:2000 IHC : 1:100-1:400 IF/ICC : 1:50-1:500 FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| Isotype | IgG2a |
| Clonality | Monoclonal |
| Clone Number | 1B5A2 |
| Conjugation | Unconjugated |
ATP5C1(ATP synthase subunit gamma, mitochondrial) is also named as ATP5C, ATP5CL1 and belongs to the ATPase gamma chain family. Some scientists reported the complete sequence of the gene for the human ATP synthase gamma subunit and described tissue-specific isoforms of the subunit generated by alternative splicing of exon 9. The liver (L) isoform differed from the heart (H) isoform by the addition of a single amino acid (asp273) at the C terminus. (PMID:8227057). Protocols Product Specific Protocols FC protocol for ATP5C1 antibody 60284-1-Ig Download protocol IF protocol for ATP5C1 antibody 60284-1-Ig Download protocol IHC protocol for ATP5C1 antibody 60284-1-Ig Download protocol WB protocol for ATP5C1 antibody 60284-1-Ig Download protocol Standard Protocols Click here to view our Standard Protocols
Publications
- Ablation of Mto1 in zebrafish exhibited hypertrophic cardiomyopathy manifested by mitochondrion RNA maturation deficiency.
Journal: Nucleic Acids Res | Authors: Qinghai Zhang | Application: WB - AKT-mediated phosphorylation of ATG4B impairs mitochondrial activity and enhances the Warburg effect in hepatocellular carcinoma cells.
Journal: Autophagy | Authors: Zhenhong Ni | Species: human | Application: WB - Small heat shock proteins operate as molecular chaperones in the mitochondrial intermembrane space
Journal: Nat Cell Biol | Authors: Elias Adriaenssens | Species: human | Application: WB - Assembly of the catalytic module and the rotor of human ATP synthase.
Journal: EMBO J | Authors: Jiaya He - Deletion of Gtpbp3 in zebrafish revealed the hypertrophic cardiomyopathy manifested by aberrant mitochondrial tRNA metabolism.
Journal: Nucleic Acids Res | Authors: Danni Chen | Species: zebrafish | Application: WB
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