PCK2 Monoclonal antibody 67676-1-Ig proteintech
$149.00
In stock
SKU
67676-1-Ig
| Catalog Number | 67676-1-Ig |
| Synonyms | 1B3F1, EC:4.1.1.32, mtPCK2, PEPCK2, PEPCK-M |
| Host | Mouse |
| Reactivity | human, mouse, rat, pig, rabbit |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, IHC, IF/ICC, FC (Intra), IP, ELISA |
| Host / Isotype | Mouse / IgG1 |
| Tested Applications — Positive WB detected in | HuH-7 cells, rat liver tissue, pig liver tissue, rabbit liver tissue, HepG2 cells, LNCaP cells, K562 cells, Neuro-2a cells, HSC-T6 cells |
| Tested Applications — Positive IP detected in | HepG2 cells |
| Tested Applications — Positive IHC detected in | human lung cancer tissue, human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Tested Applications — Positive IF/ICC detected in | MCF-7 cells |
| Tested Applications — Positive FC (Intra) detected in | HepG2 cells |
| Recommended Dilutions — Western Blot (WB) | WB : 1:2000-1:10000 |
| Recommended Dilutions — Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Recommended Dilutions — Immunohistochemistry (IHC) | IHC : 1:4000-1:16000 |
| Recommended Dilutions — Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| Recommended Dilutions — Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
| Positive WB detected in | HuH-7 cells, rat liver tissue, pig liver tissue, rabbit liver tissue, HepG2 cells, LNCaP cells, K562 cells, Neuro-2a cells, HSC-T6 cells |
| Positive IP detected in | HepG2 cells |
| Positive IHC detected in | human lung cancer tissue, human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | MCF-7 cells |
| Positive FC (Intra) detected in | HepG2 cells |
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:4000-1:16000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
| Tested Reactivity | human, mouse, rat, pig, rabbit |
| Cited Reactivity | human, mouse, rat |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | CatNo: Ag7021 Product name: Recombinant human PCK2 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 292-640 aa of BC001454 Sequence: AGKKRYVAAAFPSACGKTNLAMMRPALPGWKVECVGDDIAWMRFDSEGRLRAINPENGFFGVAPGTSATTNPNAMATIQSNTIFTNVAETSDGGVYWEGIDQPLPPGVTVTSWLGKPWKPGDKEPCAHPNSRFCAPARQCPIMDPAWEAPEGVPIDAIIFGGRRPKGVPLVYEAFNWRHGVFVGSAMRSESTAAAEHKGKIIMHDPFAMRPFFGYNFGHYLEHWLSMEGRKGAQLPRIFHVNWFRRDEAGHFLWPGFGENARVLDWICRRLEGEDSARETPIGLVPKEGALDLSGLRAIDTTQLFSLPKDFWEQEVRDIRSYLTEQVNQDLPKEVLAELEALERRVHKM Predict reactive species |
| Full Name | phosphoenolpyruvate carboxykinase 2 (mitochondrial) |
| Calculated Molecular Weight | 71 kDa |
| Observed Molecular Weight | 68 kDa |
| GenBank Accession Number | BC001454 |
| Gene Symbol | PCK2 |
| Gene ID (NCBI) | 5106 |
| RRID | AB_2882871 |
| Conjugate | Unconjugated |
| Purification Method | Protein G purification |
| UNIPROT ID | Q16822 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| FC protocol for PCK2 antibody 67676-1-Ig | Download protocol |
| IF protocol for PCK2 antibody 67676-1-Ig | Download protocol |
| IHC protocol for PCK2 antibody 67676-1-Ig | Download protocol |
| IP protocol for PCK2 antibody 67676-1-Ig | Download protocol |
| WB protocol for PCK2 antibody 67676-1-Ig | Download protocol |
| rat | WB Heliyon Tanshinone IIA attenuates fluoride-induced spinal cord injury by inhibiting ferroptosis and inflammation Authors - Qingfeng Shen View Article |
| human | IHC J Inflamm Res PCK2, a SASP-Associated Gene, Serves as an Independent Prognostic Indicator in Diffuse Large B-Cell Lymphoma. Authors - Minggui Chen View Article |
| mouse | WB PLoS One Integrated RNA-seq and scRNA-seq to explore the biological mechanisms of mitophagy-related genes in ulcerative colitis. Authors - Jianguo Ma View Article |
| Citations | 9 |
| Dilutions | WB : 1:2000-1:10000 IP : 0.5-4.0 ug for IP and 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate for WB IHC : 1:4000-1:16000 IF/ICC : 1:200-1:800 FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Clone Number | 1B3F1 |
| Conjugation | Unconjugated |
PCK2(phosphoenolpyruvate carboxykinase [GTP], mitochondrial) is also named as PEPCK2, PEPCK-M and belongs to the phosphoenolpyruvate carboxykinase [GTP] family. It catalyses the irreversible conversion of oxaloacetate to phosphoenolpyruvate, an important initial step in hepatic gluconeogenesis. Defects in PCK2 are the cause of mitochondrial phosphoenolpyruvate carboxykinase deficiency (M-PEPCKD). It has 2 isoforms produced by alternative splicing with the molecular weight of 71 kDa and 48 kDa. Protocols Product Specific Protocols FC protocol for PCK2 antibody 67676-1-Ig Download protocol IF protocol for PCK2 antibody 67676-1-Ig Download protocol IHC protocol for PCK2 antibody 67676-1-Ig Download protocol IP protocol for PCK2 antibody 67676-1-Ig Download protocol WB protocol for PCK2 antibody 67676-1-Ig Download protocol Standard Protocols Click here to view our Standard Protocols
Publications
- Black bean husk and black rice anthocyanin extracts modulated gut microbiota and serum metabolites for improvement in type 2 diabetic rats.
Journal: Food Funct | Authors: Mubai Sun | Species: rat | Application: WB - FAM136A depletion induces mitochondrial stress and reduces mitochondrial membrane potential and ATP production
Journal: FEBS Open Bio | Authors: Yushi Otsuka | Species: human | Application: WB - Integrated RNA-seq and scRNA-seq to explore the biological mechanisms of mitophagy-related genes in ulcerative colitis.
Journal: PLoS One | Authors: Jianguo Ma | Species: mouse | Application: WB - Tanshinone IIA attenuates fluoride-induced spinal cord injury by inhibiting ferroptosis and inflammation
Journal: Heliyon | Authors: Qingfeng Shen | Species: rat | Application: WB - PCK2, a SASP-Associated Gene, Serves as an Independent Prognostic Indicator in Diffuse Large B-Cell Lymphoma.
Journal: J Inflamm Res | Authors: Minggui Chen | Species: human | Application: IHC - PCK2 Inhibition Reverses Cisplatin Resistance of Non-Small Cell Lung Cancer by Triggering Ferroptosis.
Journal: FASEB J | Authors: Jing Liu - Integrated RNA-seq and scRNA-seq to explore the biological mechanisms of mitophagy-related genes in ulcerative colitis.
Journal: PLoS One | Authors: Jianguo Ma | Species: mouse | Application: WB - FAM136A depletion induces mitochondrial stress and reduces mitochondrial membrane potential and ATP production
Journal: FEBS Open Bio | Authors: Yushi Otsuka | Species: human | Application: WB - Tanshinone IIA attenuates fluoride-induced spinal cord injury by inhibiting ferroptosis and inflammation
Journal: Heliyon | Authors: Qingfeng Shen | Species: rat | Application: WB
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