DMRT1 Polyclonal antibody 28149-1-AP proteintech
$149.00
In stock
SKU
28149-1-AP
| Catalog Number | 28149-1-AP |
| Synonyms | CT154, DMT1, Doublesex- and mab-3-related transcription factor 1 |
| Host | Rabbit |
| Reactivity | human, mouse, rat |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, IHC, IF-P, ELISA |
| Host / Isotype | Rabbit / IgG |
| Tested Applications — Positive WB detected in | A549 cells, DU 145 cells, LNCaP cells, PC-3 cells, SKOV-3 cells |
| Tested Applications — Positive IHC detected in | mouse testis tissue, rat testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Tested Applications — Positive IF-P detected in | mouse testis tissue |
| Recommended Dilutions — Western Blot (WB) | WB : 1:500-1:3000 |
| Recommended Dilutions — Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Recommended Dilutions — Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| Positive WB detected in | A549 cells, DU 145 cells, LNCaP cells, PC-3 cells, SKOV-3 cells |
| Positive IHC detected in | mouse testis tissue, rat testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse testis tissue |
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| Tested Reactivity | human, mouse, rat |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CatNo: Ag28006 Product name: Recombinant human DMRT1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 119-285 aa of BC040847 Sequence: VALRRQQAQEEELGISHPIPLPSAAELLVKRENNGSNPCLMTECSGTSQPPPASVPTTAASEGRMVIQDIPAVTSRGHVENTPDLVSDSTYYSSFYQPSLFPYYNNLYNCPQYSMALAADSASGEVGNPLGGSPVKNSLRGLPGPYVPGQTGNQWQMKNMENRHAMS Predict reactive species |
| Full Name | doublesex and mab-3 related transcription factor 1 |
| Calculated Molecular Weight | 39 kDa |
| Observed Molecular Weight | 39 kDa |
| GenBank Accession Number | BC040847 |
| Gene Symbol | DMRT1 |
| Gene ID (NCBI) | 1761 |
| RRID | AB_2935466 |
| Conjugate | Unconjugated |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9Y5R6 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| IF protocol for DMRT1 antibody 28149-1-AP | Download protocol |
| IHC protocol for DMRT1 antibody 28149-1-AP | Download protocol |
| WB protocol for DMRT1 antibody 28149-1-AP | Download protocol |
| Citations | - |
| Dilutions | WB : 1:500-1:3000 IHC : 1:500-1:2000 IF-P : 1:50-1:500 |
| Isotype | IgG |
| Clonality | Polyclonal |
| Clone Number | - |
| Conjugation | Unconjugated |
DMRT1, also named DMT1, belongs to the DMRT family. DMRT1 is one of the most conserved transcription factors in a variety of species and is involved in sex determination and germ cell differentiation. DMRT1 prevents the sexual switch from male to female, represses pluripotency, and balances mitosis versus meiosis. DMRT1 also maintains spermatogenesis and replenishes mGSCs by collaborating with Plzf and Sal-like protein 4 (PMID: 33420764). DMRT1 has 3 isoforms with the molecular mass of 18, 29, and 39 kDa. Protocols Product Specific Protocols IF protocol for DMRT1 antibody 28149-1-AP Download protocol IHC protocol for DMRT1 antibody 28149-1-AP Download protocol WB protocol for DMRT1 antibody 28149-1-AP Download protocol Standard Protocols Click here to view our Standard Protocols
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