DNAJC12 Polyclonal antibody 12338-1-AP proteintech
$149.00
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SKU
12338-1-AP
| Catalog Number | 12338-1-AP |
| Synonyms | DNAJC12, J domain containing protein 1, JDP1 |
| Host | Rabbit |
| Reactivity | human and More (1) |
| Reactivity | human, mouse |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, IHC, IF, ELISA |
| Applications | WB, IHC, ELISA |
| Host / Isotype | Rabbit / IgG |
| Tested Applications — Positive WB detected in | A549 cells, BxPC-3 cells, HEK-293 cells |
| Tested Applications — Positive IHC detected in | human stomach cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Recommended Dilutions — Western Blot (WB) | WB : 1:500-1:1000 |
| Recommended Dilutions — Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Positive WB detected in | A549 cells, BxPC-3 cells, HEK-293 cells |
| Positive IHC detected in | human stomach cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CatNo: Ag2989 Product name: Recombinant human DNAJC12 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-198 aa of BC017018 Sequence: MDAILNYRSEDTEDYYTLLGCDELSSVEQILAEFKVRALECHPDKHPENPKAVETFQKLQKAKEILTNEESRARYDHWRRSQMSMPFQQWEALNDSVKTSMHWVVRGKKDLMLEESDKTHTTKMENEECNEQRERKKEELASTAEKTEQKEPKPLEKSVSPQNSDSSGFADVNGWHLRFRWSKDAPSELLRKFRNYEI Predict reactive species |
| Full Name | DnaJ (Hsp40) homolog, subfamily C, member 12 |
| Calculated Molecular Weight | 198 aa, 23 kDa |
| Observed Molecular Weight | 28 kDa |
| GenBank Accession Number | BC017018 |
| Gene Symbol | DNAJC12 |
| Gene ID (NCBI) | 56521 |
| RRID | AB_2246188 |
| Conjugate | Unconjugated |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9UKB3 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| IHC protocol for DNAJC12 antibody 12338-1-AP | Download protocol |
| WB protocol for DNAJC12 antibody 12338-1-AP | Download protocol |
| human | WB NPJ Parkinsons Dis Central biogenic amine deficiency with concomitant exploratory behavioral deficits in Dnajc12 knock-out mice Authors - Isaac Bul Deng KD Validated View Article |
| mouse | IF bioRxiv Characterization of Dnajc12 knockout mice, a model of hypodopaminergia Authors - Isaac Bul Deng View Article |
| Citations | 7 |
| Dilutions | WB : 1:500-1:1000 IHC : 1:50-1:500 |
| Isotype | IgG |
| Clonality | Polyclonal |
| Clone Number | - |
| Conjugation | Unconjugated |
Publications
- Personalized drug screening in patient-derived organoids of biliary tract cancer and its clinical application
Journal: Cell Rep Med | Authors: Xiaoxue Ren | Species: human | Application: IHC - DNAJC12 as a Mediator Between ESR1 and ERBB4 in Breast Carcinoma Cells.
Journal: Front Oncol | Authors: Mianjie Lin | Species: human | Application: WB - The co-chaperone DNAJC12 binds to Hsc70 and is upregulated by endoplasmic reticulum stress.
Journal: Cell Stress Chaperones | Authors: Choi Jin J | Species: human | Application: WB,IF - Characterization of Dnajc12 knockout mice, a model of hypodopaminergia
Journal: bioRxiv | Authors: Isaac Bul Deng | Species: mouse | Application: IF - Increased Expression of DNAJC12 is Associated with Aggressive Phenotype of Gastric Cancer.
Journal: Ann Surg Oncol | Authors: Yasuo Uno KD Validated | Species: human | Application: WB - Central biogenic amine deficiency with concomitant exploratory behavioral deficits in Dnajc12 knock-out mice
Journal: NPJ Parkinsons Dis | Authors: Isaac Bul Deng KD Validated | Species: human | Application: WB
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