DNM3 Polyclonal antibody 14737-1-AP proteintech
$149.00
In stock
SKU
14737-1-AP
| Catalog Number | 14737-1-AP |
| Synonyms | dynamin 3, Dynamin-3, EC:3.6.5.5, KIAA0820, T dynamin |
| Host | Rabbit |
| Reactivity | human, mouse, rat |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, IHC, IF/ICC, IP, ELISA |
| Host / Isotype | Rabbit / IgG |
| Tested Applications — Positive WB detected in | mouse brain tissue, fetal human brain tissue, rat brain tissue |
| Tested Applications — Positive IP detected in | mouse brain tissue |
| Tested Applications — Positive IHC detected in | mouse brain tissue, human gliomas tissue, human stomach cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Tested Applications — Positive IF/ICC detected in | HeLa cells |
| Recommended Dilutions — Western Blot (WB) | WB : 1:2000-1:12000 |
| Recommended Dilutions — Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Recommended Dilutions — Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| Recommended Dilutions — Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| Positive WB detected in | mouse brain tissue, fetal human brain tissue, rat brain tissue |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | mouse brain tissue, human gliomas tissue, human stomach cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
| Western Blot (WB) | WB : 1:2000-1:12000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CatNo: Ag6381 Product name: Recombinant human DNM3 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 206-555 aa of BC064546 Sequence: KLDLMDEGTDARDVLENKLLPLRRGYVGVVNRSQKDIDGKKDIKAAMLAERKFFLSHPAYRHIADRMGTPHLQKVLNQQLTNHIRDTLPNFRNKLQGQLLSIEHEVEAYKNFKPEDPTRKTKALLQMVQQFAVDFEKRIEGSGDQVDTLELSGGAKINRIFHERFPFEIVKMEFNEKELRREISYAIKNIHGIRTGLFTPDMAFEAIVKKQIVKLKGPSLKSVDLVIQELINTVKKCTKKLANFPRLCEETERIVANHIREREGKTKDQVLLLIDIQVSYINTNHEDFIGFANAQQRSSQVHKKTTVGNQVIRKGWLTISNIGIMKGGSKGYWFVLTAESLSWYKDDENI Predict reactive species |
| Full Name | dynamin 3 |
| Calculated Molecular Weight | 98 kDa |
| Observed Molecular Weight | 97-100 kDa |
| GenBank Accession Number | BC064546 |
| Gene Symbol | DNM3 |
| Gene ID (NCBI) | 26052 |
| RRID | AB_2093691 |
| Conjugate | Unconjugated |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9UQ16 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| IF protocol for DNM3 antibody 14737-1-AP | Download protocol |
| IHC protocol for DNM3 antibody 14737-1-AP | Download protocol |
| IP protocol for DNM3 antibody 14737-1-AP | Download protocol |
| WB protocol for DNM3 antibody 14737-1-AP | Download protocol |
| mouse | WB,IF Biol Reprod Developmental expression of the dynamin family of mechanoenzymes in the mouse epididymis. Authors - Wei Zhou View Article |
| human | WB Clin Exp Metastasis Identification and validation of genes with expression patterns inverse to multiple metastasis suppressor genes in breast cancer cell lines. Authors - Natascia Marino KD Validated View Article |
| Citations | 8 |
| Dilutions | WB : 1:2000-1:12000 IP : 0.5-4.0 ug for IP and 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate for WB IHC : 1:200-1:800 IF/ICC : 1:200-1:800 |
| Isotype | IgG |
| Clonality | Polyclonal |
| Clone Number | - |
| Conjugation | Unconjugated |
DNM3 (dynamin 3) is a member of dynamin proteins that participate in membrane rearrangements such as vesicular trafficking and phaocytosis. Three dynamin proteins encoded by different genes are present in mammals: DNM1 is mainly expressed in the central nervous system, DNM2 is ubiquitously expressed, and DNM3 is expressed chiefly in the testis and brain. Western blot using this antibody specifically detected the 100 kDa DNM3 in mouse brain. It doesn't cross-react with other DNMs. (22977254, 21671749) Protocols Product Specific Protocols IF protocol for DNM3 antibody 14737-1-AP Download protocol IHC protocol for DNM3 antibody 14737-1-AP Download protocol IP protocol for DNM3 antibody 14737-1-AP Download protocol WB protocol for DNM3 antibody 14737-1-AP Download protocol Standard Protocols Click here to view our Standard Protocols
Publications
- Modification of Crocodile Spermatozoa Refutes the Tenet That Post-testicular Sperm Maturation Is Restricted To Mammals
Journal: Mol Cell Proteomics | Authors: Brett Nixon | Application: IF - Proteomic profiling of crocodile spermatozoa refutes the tenet that post-testicular maturation is restricted to mammals.
Journal: Mol Cell Proteomics | Authors: Brett Nixon | Species: mouse | Application: IF - Dynamin regulates specific membrane fusion events necessary for acrosomal exocytosis in mouse spermatozoa.
Journal: J Biol Chem | Authors: Reid Andrew T AT | Species: mouse | Application: WB,IHC,IF - Characterization of a novel role for the dynamin mechanoenzymes in the regulation of human sperm acrosomal exocytosis.
Journal: Mol Hum Reprod | Authors: Wei Zhou | Species: human | Application: IF - Identification and validation of genes with expression patterns inverse to multiple metastasis suppressor genes in breast cancer cell lines.
Journal: Clin Exp Metastasis | Authors: Natascia Marino KD Validated | Species: human | Application: WB - Developmental expression of the dynamin family of mechanoenzymes in the mouse epididymis.
Journal: Biol Reprod | Authors: Wei Zhou | Species: mouse | Application: WB,IF
Reviews
Write Your Own Review
Related Products
Check items to add to the cart or
