CoraLite® Plus 488-conjugated DOHH Monoclonal antibody CL488-68151 proteintech
$479.00
In stock
SKU
CL488-68151
| Catalog Number | CL488-68151 |
| Synonyms | 1H5H7, EC:1.14.99.29, hDOHH, HEAT-like repeat-containing protein 1, HLRC1 |
| Host | Mouse |
| Reactivity | human, mouse, rat |
| Form | Liquid |
| Formulation | PBS, Proclin300, BSA, Glycerol |
| Applications | IF/ICC |
| Host / Isotype | Mouse / IgG1 |
| Tested Applications — Positive IF/ICC detected in | LNCaP cells |
| Recommended Dilutions — Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Positive IF/ICC detected in | LNCaP cells |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Tested Reactivity | human, mouse, rat |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | CatNo: Ag13041 Product name: Recombinant human DOHH protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 1-302 aa of BC002817 Sequence: MVTEQEVDAIGQTLVDPKQPLQARFRALFTLRGLGGPGAIAWISQAFDDDSALLKHELAYCLGQMQDARAIPMLVDVLQDTRQEPMVRHEAGEALGAIGDPEVLEILKQYSSDPVIEVAETCQLAVRRLEWLQQHGGEPAAGPYLSVDPAPPAEERDVGRLREALLDESRPLFERYRAMFALRNAGGEEAALALAEGLHCGSALFRHEVGYVLGQLQHEAAVPQLAAALARCTENPMVRHECAEALGAIARPACLAALQAHADDPERVVRESCEVALDMYEHETGRAFQYADGLEQLRGAPS Predict reactive species |
| Full Name | deoxyhypusine hydroxylase/monooxygenase |
| Calculated Molecular Weight | 33 kDa |
| Observed Molecular Weight | 30-35 kDa |
| GenBank Accession Number | BC002817 |
| Gene Symbol | DOHH |
| Gene ID (NCBI) | 83475 |
| RRID | AB_3672975 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
| Excitation Laser | Blue laser (488 nm) |
| Purification Method | Protein G purification |
| UNIPROT ID | Q9BU89 |
| Storage Buffer | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. |
| IF protocol for CL Plus 488 DOHH antibody CL488-68151 | Download protocol |
| Citations | - |
| Dilutions | IF/ICC : 1:50-1:500 |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Clone Number | 1H5H7 |
| Conjugation | CoraLite® Plus 488 |
Deoxyhypusine hydroxylase (DOHH) is the enzyme catalyzing the second step in the post-translational synthesis of hypusine [Nε-(4-amino-2-hydroxybutyl)lysine] in the eukaryotic initiation factor 5A (eIF5A). DOHH has been reported to mediate several crucial cellular functions, including cellular proliferation, differentiation and apoptosis. Moreover, previous studies have established that DOHH is highly involved in several essential biological processes driving human diseases including cancer growth, malarial drug resistance, and HIV-1 replication. In particular, the DOHH/eIF5A signaling pathway was revealed to mediate the ability of nerve growth factor to enhance neuronal growth and survival, highlighting the importance of DOHH in neuroprotection(PMID: 35007708, PMID: 16371467, PMID: 22908221, PMID: 19706422). Protocols Product Specific Protocols IF protocol for CL Plus 488 DOHH antibody CL488-68151 Download protocol Standard Protocols Click here to view our Standard Protocols
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