DOPA decarboxylase/DDC Recombinant monoclonal antibody 85811-2-RR proteintech
$149.00
In stock
SKU
85811-2-RR
| Catalog Number | 85811-2-RR |
| Synonyms | DDC, DOPA decarboxylase, AADC, Aromatic-L-amino-acid decarboxylase, EC:4.1.1.28 |
| Host | Rabbit |
| Reactivity | human, mouse |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, IF/ICC, ELISA |
| Host / Isotype | Rabbit / IgG |
| Tested Applications — Positive WB detected in | SH-SY5Y cells, mouse brain tissue, mouse kidney tissue |
| Tested Applications — Positive IF/ICC detected in | SH-SY5Y cells |
| Recommended Dilutions — Western Blot (WB) | WB : 1:5000-1:50000 |
| Recommended Dilutions — Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| Positive WB detected in | SH-SY5Y cells, mouse brain tissue, mouse kidney tissue |
| Positive IF/ICC detected in | SH-SY5Y cells |
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| Tested Reactivity | human, mouse |
| Class | Recombinant |
| Type | Antibody |
| Immunogen | CatNo: Ag0219 Product name: Recombinant human DDC protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 121-389 aa of BC008366 Sequence: WLGKMLELPKAFLNEKAGEGGGVIQGSASEATLVALLAARTKVIHRLQAASPELTQAAIMEKLVAYSSDQAHSSVERAGLIGGVKLKAIPSDGNFAMRASALQEALERDKAAGLIPFFMVATLGTTTCCSFDNLLEVGPICNKEDIWLHVDAAYAGSAFICPEFRHLLNGVEFADSFNFNPHKWLLVNFDCSAMWVKKRTDLTGAFRLDPTYLKHSHQDSGLITDYRHWQIPLGRRFRSLKMWFVFRMYGVKGLQAYIRKHVQLSHEFE Predict reactive species |
| Full Name | dopa decarboxylase (aromatic L-amino acid decarboxylase) |
| Calculated Molecular Weight | 54 kDa |
| Observed Molecular Weight | 48-50 kDa |
| GenBank Accession Number | BC008366 |
| Gene Symbol | DOPA decarboxylase |
| Gene ID (NCBI) | 1644 |
| RRID | AB_3744393 |
| Conjugate | Unconjugated |
| Purification Method | Protein A purification |
| UNIPROT ID | P20711 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| IF protocol for DOPA decarboxylase/DDC antibody 85811-2-RR | Download protocol |
| WB protocol for DOPA decarboxylase/DDC antibody 85811-2-RR | Download protocol |
| Citations | - |
| Dilutions | WB : 1:5000-1:50000 IF/ICC : 1:200-1:800 |
| Isotype | IgG |
| Clonality | Recombinant |
| Clone Number | 243125A1 |
| Conjugation | Unconjugated |
DOPA decarboxylase (DDC), also known as aromatic l-amino acid decarboxylase, belongs to the pyridoxal-dependent aminotransferase superfamily. DDC is an enzyme that converts levodopa into dopamine14, the latter being severely depleted in LBD due to the loss of dopaminergic neurons in the substantia nigra (PMID: 3374198, PMID: 28100251). DDC catalyzes the decarboxylation of L-3,4-dihydroxyphenylalanine (DOPA) to dopamine, L-5-hydroxytryptophan to serotonin and L-tryptophan to tryptamine. DDC is the cause of aromatic L-amino-acid decarboxylase deficiency (AADCD). Researches showed that Ddc is only one of the enzymes in the biosynthetic pathways for bioamines and catecholamines. Protocols Product Specific Protocols IF protocol for DOPA decarboxylase/DDC antibody 85811-2-RR Download protocol WB protocol for DOPA decarboxylase/DDC antibody 85811-2-RR Download protocol Standard Protocols Click here to view our Standard Protocols
Reviews
Write Your Own Review
Related Products
Check items to add to the cart or
