DOT1L Polyclonal antibody 28145-1-AP proteintech
$149.00
In stock
SKU
28145-1-AP
| Catalog Number | 28145-1-AP |
| Synonyms | Lysine N methyltransferase 4, KMT4, KIAA1814, H3 K79 HMTase, DOT1 like protein |
| Host | Rabbit |
| Reactivity | human, rat and More (1) |
| Reactivity | human, rat, mouse |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, IHC, ELISA |
| Applications | WB, ELISA |
| Host / Isotype | Rabbit / IgG |
| Tested Applications — Positive WB detected in | PC-12 cells |
| Recommended Dilutions — Western Blot (WB) | WB : 1:1000-1:6000 |
| Positive WB detected in | PC-12 cells |
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Tested Reactivity | human, rat |
| Cited Reactivity | mouse |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CatNo: Ag27229 Product name: Recombinant human DOT1L protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 511-698 aa of NM_032482 Sequence: MLQELLGQEKEKNAQLLGAAQQLLSHCQAQKEEIRRLFQQKLDELGVKALTYNDLIQAQKEISAHNQQLREQSEQLEQDNRALRGQSLQLLKARCEELQLDWATLSLEKLLKEKQALKSQISEKQRHCLELQISIVELEKSQRQQELLQLKSCVPPDDALSLHLRGKGALGRELEPDASRLHLELDCTK Predict reactive species |
| Full Name | DOT1-like, histone H3 methyltransferase (S. cerevisiae) |
| Calculated Molecular Weight | 185 kDa |
| Observed Molecular Weight | 185~200 kDa |
| GenBank Accession Number | NM_032482 |
| Gene Symbol | DOT1L |
| Gene ID (NCBI) | 84444 |
| RRID | AB_3742278 |
| Conjugate | Unconjugated |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8TEK3 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| WB protocol for DOT1L antibody 28145-1-AP | Download protocol |
| mouse | WB,IHC Eur J Pharmacol Inhibition of Methyltransferase DOT1L alleviates anti-tuberculosis drug-induced liver injury by inhibiting p53-Bax/Bcl-2 pathway. Authors - Li Jinfeng View Article |
| Citations | 1 |
| Dilutions | WB : 1:1000-1:6000 |
| Isotype | IgG |
| Clonality | Polyclonal |
| Clone Number | - |
| Conjugation | Unconjugated |
DOT1L (Disruptor of telomeric silencing 1-like) is the only known histone methyltransferse catalyzing H3K79 methylation which is an activating mark for gene transcription by promoting transcriptional elongation (PMID: 34187895). DOT1L forms a complex with AF10/AF17 and ENL/AF9, is dysregulated in mixed-lineage leukemia (MLLr), and is believed to regulate transcriptional elongation without direct evidence. DOT1L has been reported to directly interact with RNAPII phosphorylated on Ser2 and/or Ser5 of the C-terminal domain (CTD) of its largest subunit. Loss of Dot1L results in yolk sac angiogenesis defects and embryonic lethality (PMID: 18787701). Protocols Product Specific Protocols WB protocol for DOT1L antibody 28145-1-AP Download protocol Standard Protocols Click here to view our Standard Protocols
Publications
- Inhibition of Methyltransferase DOT1L alleviates anti-tuberculosis drug-induced liver injury by inhibiting p53-Bax/Bcl-2 pathway.
Journal: Eur J Pharmacol | Authors: Li Jinfeng | Species: mouse | Application: WB,IHC
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