MKP-2/DUSP4 Polyclonal antibody 10739-1-AP proteintech
$149.00
In stock
SKU
10739-1-AP
| Catalog Number | 10739-1-AP |
| Synonyms | DUSP4, MKP-2, dual specificity phosphatase 4, EC:3.1.3.16, EC:3.1.3.48 |
| Host | Rabbit |
| Reactivity | human, mouse |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, IHC, IP, ELISA |
| Applications | WB, IP, ELISA |
| Host / Isotype | Rabbit / IgG |
| Tested Applications — Positive WB detected in | HCT 116 cells, SK-BR-3 cells |
| Tested Applications — Positive IP detected in | MDA-MB-231 cells |
| Tested Applications — Positive FC (Intra) detected in | MDA-MB-231 cells |
| Recommended Dilutions — Western Blot (WB) | WB : 1:1000-1:4000 |
| Recommended Dilutions — Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Recommended Dilutions — Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
| Positive WB detected in | HCT 116 cells, SK-BR-3 cells |
| Positive IP detected in | MDA-MB-231 cells |
| Positive FC (Intra) detected in | MDA-MB-231 cells |
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CatNo: Ag1206 Product name: Recombinant human MKP-2 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-394 aa of BC014565 Sequence: MVTMEELREMDCSVLKRLMNRDENGGGAGGSGSHGTLGLPSGGKCLLLDCRPFLAHSAGYILGSVNVRCNTIVRRRAKGSVSLEQILPAEEEVRARLRSGLYSAVIVYDERSPRAESLREDSTVSLVVQALRRNAERTDICLLKGGYERFSSEYPEFCSKTKALAAIPPPVPPSATEPLDLGCSSCGTPLHDQGGPVEILPFLYLGSAYHAARRDMLDALGITALLNVSSDCPNHFEGHYQYKCIPVEDNHKADISSWFMEAIEYIDAVKDCRGRVLVHCQAGISRSATICLAYLMMKKRVRLEEAFEFVKQRRSIISPNFSFMGQLLQFESQVLATSCAAEAASPSGPLRERGKTPATPTSQFVFSFPVSVGVHSAPSSLPYLHSPITTSPSC Predict reactive species |
| Full Name | dual specificity phosphatase 4 |
| Calculated Molecular Weight | 43 kDa |
| Observed Molecular Weight | 40-43 kDa |
| GenBank Accession Number | BC014565 |
| Gene Symbol | DUSP4 |
| Gene ID (NCBI) | 1846 |
| RRID | AB_2095178 |
| Conjugate | Unconjugated |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q13115 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| FC protocol for MKP-2/DUSP4 antibody 10739-1-AP | Download protocol |
| IP protocol for MKP-2/DUSP4 antibody 10739-1-AP | Download protocol |
| WB protocol for MKP-2/DUSP4 antibody 10739-1-AP | Download protocol |
| mouse | WB Nat Commun Phosphoglycerate dehydrogenase-mediated serine reprogramming aggravates macrophage hyperinflammation in murine Pseudomonas aeruginosa pneumonia. Authors - Rong Chen View Article |
| human | WB Cell Stress Chaperones JNK Signaling Dominance in Hyperthermia Authors - Atsushi Enomoto View Article |
| Citations | 5 |
| Dilutions | WB : 1:1000-1:4000 IP : 0.5-4.0 ug for IP and 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate for WB FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
| Isotype | IgG |
| Clonality | Polyclonal |
| Clone Number | - |
| Conjugation | Unconjugated |
DUSP4, also named as MKP-2, is a member of the mitogen-activated protein (MAP) kinase phosphatase family which has been suggested to play an important role in the feedback control of MAP kinase-mediated gene expression. DUSP4 preferentially inactivates ERK1, ERK2 and JNK, is expressed in a variety of tissues, and is localized in the nucleus. Protocols Product Specific Protocols FC protocol for MKP-2/DUSP4 antibody 10739-1-AP Download protocol IP protocol for MKP-2/DUSP4 antibody 10739-1-AP Download protocol WB protocol for MKP-2/DUSP4 antibody 10739-1-AP Download protocol Standard Protocols Click here to view our Standard Protocols
Publications
- Compound haploinsufficiency of Dok2 and Dusp4 promotes lung tumorigenesis.
Journal: J Clin Invest | Authors: Ming Chen KO Validated | Species: mouse | Application: WB,IHC - NR4A1 enhances MKP7 expression to diminish JNK activation induced by ROS or ER-stress in pancreatic β cells for surviving.
Journal: Cell Death Discov | Authors: Ze-Qing Pu | Species: mouse | Application: WB - JNK Signaling Dominance in Hyperthermia
Journal: Cell Stress Chaperones | Authors: Atsushi Enomoto | Species: human | Application: WB - DUSP4 mitigates doxorubicin-induced cardiotoxicity via the p38 MAPK/MK2 signaling pathway.
Journal: Biochim Biophys Acta Mol Cell Res | Authors: Lijie Deng KD Validated | Species: mouse | Application: WB - Phosphoglycerate dehydrogenase-mediated serine reprogramming aggravates macrophage hyperinflammation in murine Pseudomonas aeruginosa pneumonia.
Journal: Nat Commun | Authors: Rong Chen | Species: mouse | Application: WB
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