Di-Methyl-Histone H3 (Lys56) Recombinant monoclonal antibody 82828-12-RR proteintech
$149.00
In stock
SKU
82828-12-RR
| Catalog Number | 82828-12-RR |
| Synonyms | Di Methyl Histone H3, Di-Methyl Histone H3 (Lys56), Di-Methyl Histone H3 Lys56, Di-Methyl Histone-H3 Lys56, Di-Methyl-Histone H3 |
| Host | Rabbit |
| Reactivity | human, mouse, rat |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, IF/ICC, Dot Blot, ELISA |
| Host / Isotype | Rabbit / IgG |
| Tested Applications — Positive WB detected in | HeLa cells, HepG2 cells, NIH/3T3 cells, HSC-T6 cells |
| Tested Applications — Positive IF/ICC detected in | HepG2 cells, NIH/3T3 cells |
| Tested Applications — Positive Dot Blot detected in | peptide |
| Recommended Dilutions — Western Blot (WB) | WB : 1:500-1:2000 |
| Recommended Dilutions — Immunofluorescence (IF)/ICC | IF/ICC : 1:250-1:1000 |
| Positive WB detected in | HeLa cells, HepG2 cells, NIH/3T3 cells, HSC-T6 cells |
| Positive IF/ICC detected in | HepG2 cells, NIH/3T3 cells |
| Positive Dot Blot detected in | peptide |
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:250-1:1000 |
| DOT BLOT | DOT BLOT : 1:10-1:100 |
| Tested Reactivity | human, mouse, rat |
| Class | Recombinant |
| Type | Antibody |
| Immunogen | Peptide Predict reactive species |
| Full Name | histone cluster 1, H3a |
| Observed Molecular Weight | 15-18 kDa |
| GenBank Accession Number | BC066245 |
| Gene Symbol | HIST1H3A |
| Gene ID (NCBI) | 8350 |
| RRID | AB_3743436 |
| Conjugate | Unconjugated |
| Purification Method | Protein A purification |
| UNIPROT ID | P68431 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| IF protocol for Di-Methyl-Histone H3 (Lys56) antibody 82828-12-RR | Download protocol |
| WB protocol for Di-Methyl-Histone H3 (Lys56) antibody 82828-12-RR | Download protocol |
| Citations | - |
| Dilutions | WB : 1:500-1:2000 IF/ICC : 1:250-1:1000 DOT BLOT : 1:10-1:100 |
| Isotype | IgG |
| Clonality | Recombinant |
| Clone Number | 243027A1 |
| Conjugation | Unconjugated |
Histones, including H1/H5 (linker histones), H2, H3, and H4 (core histones), are nucleic proteins which interact with DNA to form the nucleosomes and play important roles in gene regulation and DNA replication. Histone proteins are highly post-translationally modified while Histone H3 is the most extensively modified.The NSD family histone methyltransferases, including NSD1, NSD2 and NSD3, play crucial roles in chromatin regulation and are implicated in oncogenesis (PMID: 33361816). Protocols Product Specific Protocols IF protocol for Di-Methyl-Histone H3 (Lys56) antibody 82828-12-RR Download protocol WB protocol for Di-Methyl-Histone H3 (Lys56) antibody 82828-12-RR Download protocol Standard Protocols Click here to view our Standard Protocols
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