ECE1 Polyclonal antibody 26088-1-AP proteintech
$149.00
In stock
SKU
26088-1-AP
| Catalog Number | 26088-1-AP |
| Synonyms | ECE, ECE 1, ECE1, endothelin converting enzyme 1 |
| Host | Rabbit |
| Reactivity | human and More (1) |
| Reactivity | human, rat |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, IHC, IF, ELISA |
| Applications | WB, IHC, ELISA |
| Host / Isotype | Rabbit / IgG |
| Tested Applications — Positive WB detected in | HeLa cells, A549 cells |
| Tested Applications — Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Recommended Dilutions — Western Blot (WB) | WB : 1:500-1:2000 |
| Recommended Dilutions — Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Positive WB detected in | HeLa cells, A549 cells |
| Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Tested Reactivity | human |
| Cited Reactivity | human, rat |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CatNo: Ag23144 Product name: Recombinant human ECE1 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 335-425 aa of BC126257 Sequence: TAAELQTLAPAINWLPFLNTIFYPVEINESEPIVVYDKEYLEQISTLINTTDRCLLNNYMIWNLVRKTSSFLDQRFQDADEKFMEVMYGTK Predict reactive species |
| Full Name | endothelin converting enzyme 1 |
| Observed Molecular Weight | 125-135 kDa |
| GenBank Accession Number | BC126257 |
| Gene Symbol | ECE1 |
| Gene ID (NCBI) | 1889 |
| RRID | AB_2880371 |
| Conjugate | Unconjugated |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P42892 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| IHC protocol for ECE1 antibody 26088-1-AP | Download protocol |
| WB protocol for ECE1 antibody 26088-1-AP | Download protocol |
| rat | IF Adv Sci (Weinh) Astrocytic ET-1 System Determines Microglia Phenotype Following Spinal Cord Injury Authors - Bingqiang He View Article |
| human | WB Laryngoscope Investig Otolaryngol Hypoxia-inducible factor-1α contributes to the proliferation of cholesteatoma keratinocytes through regulating endothelin converting enzyme 1 expression Authors - Nie Chen KD Validated View Article |
| Jaouhara (Verified Customer) (12-05-2023) | Specific antibody. Validated in immunofluorescence and immunohistochemistry. Good signal. Applications: Immunohistochemistry, Immunofluorescence Cell Tissue Type: Chondrocytes |
| Citations | 3 |
| Dilutions | WB : 1:500-1:2000 IHC : 1:50-1:500 |
| Isotype | IgG |
| Clonality | Polyclonal |
| Clone Number | - |
| Conjugation | Unconjugated |
Publications
- Astrocytic ET-1 System Determines Microglia Phenotype Following Spinal Cord Injury
Journal: Adv Sci (Weinh) | Authors: Bingqiang He | Species: rat | Application: IF - Characterization of endothelin-converting enzyme 1 as a key enzyme in the multienzyme Aβ degradation pathway.
Journal: Proc Natl Acad Sci U S A | Authors: Irem Ulku KD Validated | Species: human | Application: WB - Hypoxia-inducible factor-1α contributes to the proliferation of cholesteatoma keratinocytes through regulating endothelin converting enzyme 1 expression
Journal: Laryngoscope Investig Otolaryngol | Authors: Nie Chen KD Validated | Species: human | Application: WB
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